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Journal of Plant Research

, Volume 121, Issue 2, pp 245–249 | Cite as

Split-root study of autoregulation of nodulation in the model legume Lotus japonicus

  • Akihiro Suzuki
  • Hisatoshi Hara
  • Tomoyo Kinoue
  • Mikiko Abe
  • Toshiki Uchiumi
  • Ken-ichi Kucho
  • Shiro Higashi
  • Ann M. Hirsch
  • Susumu Arima
Short Communication

Abstract

We used a split-root system to determine the timing for induction of the autoregulation of nodulation (AUT) in Lotus japonicus (Regel) Larsen after inoculation with Mesorhizobium loti. The signal took at least five days for full induction of AUT and inhibition of infection thread formation. Strain ML108 (able to nodulate but unable to fix nitrogen) induced full AUT, but ML101 (unable to nodulate or to fix nitrogen) did not induce autoregulation. These results indicate that Nod factor-producing strains induce AUT, but that the nitrogen fixed by rhizobia and supplied to the plant as ammonia does not elicit the AUT in L. japonicus.

Keywords

Lotus japonicus Autoregulation Nodulation Symbiosis Nitrogen fixation Split-root system 

Notes

Acknowledgments

Lotus japonicus Miyakojima MG-20 seeds were provided by University of Miyazaki, through the National Bio-Resource Project (NBRP) of the MEXT, Japan. This work was supported in part by the Special Coordination Funds for Promoting Science and Technology from the Ministry of Education, Culture, Sports, Science and Technology in Japan.

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Copyright information

© The Botanical Society of Japan and Springer 2008

Authors and Affiliations

  • Akihiro Suzuki
    • 1
  • Hisatoshi Hara
    • 2
  • Tomoyo Kinoue
    • 3
  • Mikiko Abe
    • 3
  • Toshiki Uchiumi
    • 3
  • Ken-ichi Kucho
    • 3
  • Shiro Higashi
    • 3
  • Ann M. Hirsch
    • 4
  • Susumu Arima
    • 1
  1. 1.Department of Environmental Sciences, Faculty of AgricultureSaga UniversitySagaJapan
  2. 2.Graduate School of Science and TechnologyKagoshima UniversityKagoshimaJapan
  3. 3.Department of Chemistry and Bioscience, Faculty of ScienceKagoshima UniversityKagoshimaJapan
  4. 4.Department of Molecular, Cell and Developmental Biology and Molecular Biology InstituteUniversity of California-Los AngelesLos AngelesUSA

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