Journal of Plant Research

, Volume 121, Issue 2, pp 215–226 | Cite as

Molecular cloning, expression profiling and trans-activation property studies of a DREB2-like gene from chrysanthemum (Dendranthema vestitum)

  • Liqing Liu
  • Kai Zhu
  • Yanfang Yang
  • Jian Wu
  • Fadi Chen
  • Deyue Yu
Regular Paper

Abstract

Dehydration responsive element binding (DREB) proteins are important transcription factors in plant stress response and signal transduction. In this study, a DREB homolog gene, DvDREB2A, was isolated from chrysanthemum (Dendranthema vestitum) by reverse transcriptase-polymerase chain reaction (RT-PCR) and rapid amplification of cDNA ends (RACE) methods. It contained an open reading frame (ORF) of 1,471 bp encoding 366 amino acid residues and was classified as a DREB2 subfamily member based on multiple sequence alignment. The predicted protein sequence contained a typical AP2/EREBP DNA-binding domain near the N-terminal region. In yeast one-hybrid analysis DvDREB2A protein was specifically bound to DRE elements (core sequence, A/GCCGAC) and activated the expression of the reporter HIS3 and LacZ. Transient expression experiment suggested that DvDREB2A protein was localized to the nucleus of onion epidermis cells. Quantitative real-time PCR (QRT-PCR) experiments showed that expression level of DvDREB2A was significantly affected by heat, low temperature, drought, abscisic acid (ABA) and high salinity treatments. These results indicated that the DvDREB2A gene is a new member of the DREB transcription factors, which may play an important role in providing tolerance to environmental stresses.

Keywords

Abiotic stresses Chrysanthemum DREB Quantitative real-time PCR 

Notes

Acknowledgments

We are greatly thankful to Prof. Qiang Liu for kindly providing the yeast one-hybrid system. Prof. Shou Yi Chen is thankful for important research advice. This work was supported by an award grant for outstanding scholars from the Ministry of Education of China.

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Copyright information

© The Botanical Society of Japan and Springer 2008

Authors and Affiliations

  • Liqing Liu
    • 1
  • Kai Zhu
    • 1
  • Yanfang Yang
    • 1
  • Jian Wu
    • 1
  • Fadi Chen
    • 2
  • Deyue Yu
    • 1
  1. 1.National Center for Soybean Improvement, National Key Laboratory of Crop Genetics and Germplasm EnhancementNanjing Agricultural UniversityNanjingPeople’s Republic of China
  2. 2.College of HorticultureNanjing Agricultural UniversityNanjingPeople’s Republic of China

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