Comparative analysis of telomeric restriction fragment lengths in different tissues of Ginkgo biloba trees of different age
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Ginkgo trees of four different ages were selected as experimental material. Telomeric restriction fragment (TRF) lengths, as an indicator of telomere length, were determined for different tissues by Southern hybridization analysis. Statistical analysis was performed to compare two aspects of TRF length. By determining TRF lengths for different tissues for each age, a latent tendency was found. TRF length varied from short to long in these tissues in the order microspore < embryonal callus < leaf < branchlet. TRF lengths for leaf tissue and branchlet tissue were dissimilar for female and male mature trees, although this difference between TRF lengths for the two sexes was not statistically significant. Evaluation of TRF lengths for each tissue for trees of all four ages revealed TRF lengths increased with age to some extent. Different rates of change were found for leaf tissue and for branchlet tissue, although tendencies to increase were not linear for either. Finally, a simple mathematical model was formulated to describe the relationship between telomere length and age for Ginkgo biloba L.
KeywordsAge Ginkgo biloba L. Telomere TRF length (TRFL)
The authors thank Lu Yao, Xu Wang, and members of the Botany laboratory for support during data analysis and many helpful discussions, and Renyuan Zhu and Hongtao Wu for support during sample collection in the Wofo Temple of the Beijing Botanical Garden. The authors also thank Qingyin Zeng, Hailing Yang, and Pengjun Cheng for revising the manuscript. This work was supported by the National Natural Science Foundation of China (Grant No.30571473).
- Biswas C, Johri BM (1997) The gymnosperms, Narosa Publishing House New DelhiGoogle Scholar
- Bonani G, Ivy SD, Hajdas I, Niklaus TR, Suter M (1994) AMS 14C age determinations of tissue, bone and grass samples from the Otztal ice man. Radiocarbon 36(2):247–250Google Scholar
- Liu D, Lu H, Ji F, Li F, Guo H (2005) Cloning and analysis of the telomere-associated sequences of Ginkgo biloba L. For Stud China 7:7–10Google Scholar
- Muller HJ (1938) The remaking of chromosomes. Collecting Net 8:182–195, 198Google Scholar
- Nakamura K, Izumiyama-Shimomura N, Sawabe M, Arai T, Aoyagi Y, Fujiwara M, Tsuchiya E, Kobayashi Y, Kato M, Oshimura M, Sasajima K, Nakachi K, Takubo K (2002) Comparative analysis of telomere lengths and erosion with age in human epidermis and lingual epithelium. J Invest Dermatol 119:1014–1019PubMedCrossRefGoogle Scholar
- Rogers SO, Bendich AJ (1988) Extraction of DNA from plant tissues. Plant Mol Biol Manual A6:1–10Google Scholar
- Wu KS, Tanksley SD (1993) Genetic and physical mapping of telomeres and macrosatellites of rice. Plant Mol Biol 22:861–872Google Scholar