Journal of Plant Research

, Volume 120, Issue 4, pp 491–500

Chloroplast DNA phylogeography of Pedicularis ser. Gloriosae (Orobanchaceae) in Japan

Regular Paper

DOI: 10.1007/s10265-007-0083-2

Cite this article as:
Fujii, N. J Plant Res (2007) 120: 491. doi:10.1007/s10265-007-0083-2

Abstract

Phylogeographic analyses using chloroplast DNA (cpDNA) variation were performed for Pedicularis ser. Gloriosae (Orobanchaceae). Eighty-one plants of 18 populations of 6 species (P. gloriosa, P. iwatensis, P. nipponica, P. ochiaiana, P. sceptrum-carolinum and P. grandiflora) were analyzed. Fifteen distinct haplotypes were identified based on six cpDNA regions: the intergenic spacer between the trnT and trnL 3′exon, trnL 3′exon-trnF, atpB-rbcL, accDpsaI, the rpl16 intron and the trnK region (including the matK gene). Via phylogenetic analyses of the haplotypes, two continental species, P. sceptrum-carolinum and P. grandiflora, were placed at the most ancestral position in the trees. The former species is widely distributed in the Eurasian continent, and the latter is distributed in Far East Asia. Two robust major cpDNA clades (clades I and II) were revealed in the Japanese archipelago, although the statistical values of monophyly of these clades were weak. Clade I included the haplotypes (A-1, A-2, B-1, B-2 and J) of three species (P. gloriosa, P. iwatensis and P. ochiaiana), and Clade II included seven haplotypes (C-D, E-1, E-2 and F-H) of P. nipponica. These results suggest that this series originated on the Eurasian continent and that subsequently populations at the eastern edge of the continent differentiated into the two Japanese lineages.

Keywords

Chloroplast DNA Endemic taxa Japanese archipelago Orobanchaceae Pedicularis ser. Gloriosae Phylogeography 

Supplementary material

Copyright information

© The Botanical Society of Japan and Springer 2007

Authors and Affiliations

  1. 1.Makino Herbarium, Graduate School of Science, Tokyo Metropolitan UniversityTokyoJapan

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