Journal of Plant Research

, Volume 120, Issue 2, pp 229–236 | Cite as

Developmental processes of achlorophyllous orchid, Epipogium roseum: from seed germination to flowering under symbiotic cultivation with mycorrhizal fungus

  • Takahiro Yagame
  • Masahide Yamato
  • Masahiro Mii
  • Akira Suzuki
  • Koji Iwase
Regular Paper


We have achieved the symbiotic cultivation of an apparently achlorophyllous orchid, Epipogium roseum Lindl., with a mycorrhizal fungus isolated from an underground organ of this orchid. Although the seed germination rate was extremely low, subsequent growth from protocorm to flowering was induced in a medium containing volcanic soils and sawdust. Stolons elongated from each protocorm, and rhizomes were formed at certain intervals on the stolons. Some of the rhizomes developed into a coralloid form, and tubers were formed from the coralloid rhizomes. The coralloid rhizomes degenerated concurrently with maturation of the tubers. Six months after seed sowing, around 80 tubers were produced from a single protocorm. An inflorescence appeared from each of the large tubers, and the process to flowering was observed in one of these. Consequently, the developmental processes from seed to flowering in E. roseum was clearly revealed in this study.


Achlorophyllous orchid Coprinaceae Epipogium roseum Mycorrhizal fungus Symbiotic cultivation 



We thank Dr. Toshimitsu Fukiharu, Natural History Museum and Institute, Chiba, for his kind gift of C. micaceus isolate1380.


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Copyright information

© The Botanical Society of Japan and Springer 2006

Authors and Affiliations

  • Takahiro Yagame
    • 1
    • 2
  • Masahide Yamato
    • 3
  • Masahiro Mii
    • 4
  • Akira Suzuki
    • 5
  • Koji Iwase
    • 3
    • 6
  1. 1.Division of Advanced Bio-resources Sciences, Graduate School of Science and TechnologyChiba UniversityInage-ku, ChibaJapan
  2. 2.Orchid Museum, TakamoriShimoina, NaganoJapan
  3. 3.Biological Environment Institute, Research and Development DepartmentThe General Environmental Technos Co. Ltd.Uji, KyotoJapan
  4. 4.Laboratory of Plant Cell Technology, Faculty of HorticultureChiba UniversityMatsudo, ChibaJapan
  5. 5.Department of Biology, Faculty of EducationChiba University Inage-ku, ChibaJapan
  6. 6.Fungus/Mushroom Resource and Research Center, Faculty of AgricultureTottori UniversityTottoriJapan

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