Amaranthin in feather cockscombs is synthesized via glucuronylation at the cyclo-DOPA glucoside step in the betacyanin biosynthetic pathway
- 206 Downloads
Uridine 5′-diphosphate (UDP)-glucuronic acid: cyclo-DOPA 5-glucoside glucuronosyltransferase activity was detected in a crude extract prepared from the purple flowers of feather cockscombs. This suggests that the glucuronic acid moiety of amaranthin and its derivatives may be introduced at the cyclo-DOPA glucoside step, but not at the betanidin glucoside step.
KeywordsAmaranthin Betacyanin cyclo-DOPA glucoside Feather cockscombs Glucuronosyltransferase
This work was supported by a Grant-in-Aid for Scientific Research from the Ministry of Education, Culture, Sports, Science and Technology of Japan (15657008) and by the National Institute of Floricultural Science (NIFS), the National Agricultural Research Organization (NARO), and the Japan Food Chemical Research Foundation.
- Bokern M, Heuer S, Strack D (1992) Hydroxycinnamic acid transferase in the biosynthesis of acylated betacyanins: purification and characterization from cell cultures of Chenopodium rubrum and occurrence in some other members of the Caryophyllales. Bot Acta 105:146–151Google Scholar
- Sawada S, Suzuki H, Ichimaida F, Yamaguchi MA, Iwashita T, Fukui Y, Hemmi H, Nishino T, Nakayama T (2005) UDP-glucuronic acid: anthocyanin glucuronosyltransferase from red daisy (Bellis perennis) flowers. Enzymology and phylogenetics of a novel glucuronosyltransferase involved in flower pigment biosynthesis. J Biol Chem 280:899–906CrossRefPubMedGoogle Scholar
- Suzuki H, Nakayama T, Yonekura-Sakakibara K, Fukui Y, Nakamura N, Nakao M, Tanaka Y, Yamaguchi MA, Kusumi T, Nishino T (2001) Malonyl-CoA: anthocyanin 5-O-glucoside-6′′′-O-malonyltransferase from scarlet sage (Salvia splendens) flowers. Enzyme purification, gene cloning, expression, and characterization. J Biol Chem 276:49013–49019CrossRefPubMedGoogle Scholar
- Suzuki H, Sawada S, Watanabe K, Nagae S, Yamaguchi MA, Nakayama T, Nishino T (2004) Identification and characterization of a novel anthocyanin malonyltransferase from scarlet sage (Salvia splendens) flowers: an enzyme that is phylogenetically separated from other anthocyanin acyltransferases. Plant J 38:994–1003CrossRefPubMedGoogle Scholar
- Yonekura-Sakakibara K, Tanaka Y, Fukuchi-Mizutani M, Fujiwara H, Fukui Y, Ashikari T, Murakami Y, Yamaguchi M, Kusumi T (2000) Molecular and biochemical characterization of a novel hydroxycinnamoyl-CoA: anthocyanin 3-O-glucoside-6′′-O-acyltransferase from Perilla frutescens. Plant Cell Physiol 41:495–502PubMedGoogle Scholar