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Journal of Plant Research

, Volume 118, Issue 4, pp 271–284 | Cite as

Molecular phylogenetics of subtribe Aeridinae (Orchidaceae): insights from plastid matK and nuclear ribosomal ITS sequences

  • Topik Hidayat 
  • Tomohisa Yukawa
  • Motomi Ito
Original Article

Abstract

We conducted phylogenetic analyses using two DNA sequence data sets derived from matK, the maturase-coding gene located in an intron of the plastid gene trnK, and the internal transcribed spacer region of 18S–26S nuclear ribosomal DNA to examine relationships in subtribe Aeridinae (Orchidaceae). Specifically, we investigated (1) phylogenetic relationships among genera in the subtribe, (2) the congruence between previous classifications of the subtribe and the phylogenetic relationships inferred from the molecular data, and (3) evolutionary trends of taxonomically important characters of the subtribe, such as pollinia, a spurred lip, and a column foot. In all, 75 species representing 62 genera in subtribe Aeridinae were examined. Our analyses provided the following insights: (1) monophyly of subtribe Aeridinae was tentatively supported in which 14 subclades reflecting phylogenetic relationships can be recognized, (2) results are inconsistent with previous classifications of the subtribe, and (3) repeated evolution of previously emphasized characters such as pollinia number and apertures, length of spur, and column foot was confirmed. It was found that the inconsistencies are mainly caused by homoplasy of these characters. At the genus level, Phalaenopsis, Cleisostoma, and Sarcochilus are shown to be non-monophyletic.

Keywords

Aeridinae ITS matK Molecular phylogenetics Orchidaceae 

Notes

Acknowledgements

We gratefully acknowledge Dedy Darnaedi and Dwi Murti Puspitaningtyas for granting a permit to sample some materials; Pheravut Wongsawad and Haji Nazarudin for guidance during our fieldwork in Thailand and Malaysia; and Koichi Kita, Tomoko Fujimoto, Seishiro Aoki, and Daisuke Yoshimoto for technical assistance. We would like to thank Mark W. Chase and an anonymous reviewer for helpful comments and criticisms on the manuscript. We also thank Kazuhiro Suzuki for skillful cultivation of the plants. This study was partly supported by a Grant-in-Aid for Scientific Research from JSPS (MI).

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Copyright information

© The Botanical Society of Japan and Springer-Verlag 2005

Authors and Affiliations

  1. 1.Department of Biological Science, Graduate School of ScienceThe University of TokyoTokyoJapan
  2. 2.Department of Biological Education, Faculty of Math and Science EducationIndonesia University of Education (UPI Bandung)BandungIndonesia
  3. 3.Tsukuba Botanical GardenNational Science MuseumTsukubaJapan
  4. 4.Department of General Systems Sciences, Graduate School of Arts and SciencesThe University of TokyoTokyo 153-8902Japan

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