Journal of Plant Research

, Volume 118, Issue 1, pp 37–41

Nuclear DNA content in the genus Hepatica (Ranunculaceae)

  • Tomoo Mabuchi
  • Hisashi Kokubun
  • Masahiro Mii
  • Toshio Ando
Original Article

Abstract

Using flow cytometry, we measured the nuclear DNA contents of all known taxa in Hepatica. Nuclear DNA content of Hepatica falconeri (diploid, crenate leaf lobes) was significantly lower than that of diploid entire species. Among the tetraploid species, crenate species had lower DNA contents than the entire taxon H. nobilis var. pubescens. The DNA content of the tetraploid species was more than double that of the diploid species among the same leaf-type groups.

Keywords

Anemone Flow cytometry Hepatica PI/DAPI ratio Ranunculaceae Speciation 

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Copyright information

© The Botanical Society of Japan and Springer-Verlag 2005

Authors and Affiliations

  • Tomoo Mabuchi
    • 1
  • Hisashi Kokubun
    • 2
  • Masahiro Mii
    • 3
  • Toshio Ando
    • 3
  1. 1.2-11-5 Sagami-ohnoSagamihara, KanagawaJapan
  2. 2.Graduate School of Science and TechnologyChiba UniversityChibaJapan
  3. 3.Faculty of HorticultureChiba UniversityChibaJapan

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