Abstract
Molecular variations of Spiranthes sinensis Ames var. australis (R.Br.) H. Hara et Kitam. ex Kitam. in Japan were examined to evaluate the validity of the seasonally differentiated groups and a dwarf form of the species, which is endemic to Yakushima Island, Japan. Sequence differences in the plastid trnL-F locus clearly distinguished Japanese S. sinensis var. australis from S. sinensis var. sinensis collected from Ryukyu. In contrast, the trnL-F sequence of S. sinensis var. australis from Sabah, Malaysia, clearly differed from that of Japanese S. sinensis var. australis, suggesting genetic heterogeneity of Spiranthes sinensis var. australis in Asia. Moreover, a molecular analysis based on the sequences of nuclear ITS1 regions indicated that there are two major groups of S. sinensis var. australis in Japan, with a geographic distribution boundary on Kyushu Island. However, the trnL-F and ITS1 sequences did not support the genetic differentiation of the seasonally differentiated groups or the dwarf form from the other Japanese individuals. Based on these molecular data, the systematic treatment of physiological and morphological variations in the Japanese population of S. sinensis. var. australis is discussed.
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Acknowledgements
The author thanks the curators of the TI, TNS, KYO, and TKPM herbaria who kindly permitted the author to examine herbarium specimens of S. sinensis. The author thanks Dr. Futoshi Miyamoto at Tokyo University of Agriculture, Japan; Dr. Jun Yokoyama at Tohoku University, Japan; Drs. Hidetoshi Nagamasu and Akiyo Naiki at Kyoto University; Prof. Hiroshi Okada at Osaka City University, Japan; and Prof. Dr. Maryati Mohamed at Universitas Malaysia Sabah, Malaysia, for their help with sample collection; Ms. Mutsuko Nakajima for her helpful suggestions; and Prof. Hideaki Ohba at University of Tokyo for his helpful discussions and assistance with Latin descriptions. This study was supported by a grant from the SOKENDAI group research project of the Graduate University for Advanced Studies and a Grant-in-Aid from the Ministry of Education, Culture, Sports, Science, and Technology of Japan.
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Tsukaya, H. Molecular variation of Spiranthes sinensis (Orchidaceae) in Japan, with special reference to systematic treatment of seasonally differentiated groups and a dwarf form, f. gracilis, from Yakushima Island. J Plant Res 118, 13–18 (2005). https://doi.org/10.1007/s10265-004-0186-y
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DOI: https://doi.org/10.1007/s10265-004-0186-y