Journal of Plant Research

, Volume 117, Issue 2, pp 109–120 | Cite as

Molecular phylogeny of monocotyledons inferred from combined analysis of plastid matK and rbcL gene sequences

  • Minoru N. Tamura
  • Jun Yamashita
  • Shizuka Fuse
  • Masatake Haraguchi
Original Article

Abstract

Using matK and rbcL sequences (3,269 bp in total) from 113 genera of 45 families, we conducted a combined analysis to contribute to the understanding of major evolutionary relationships in the monocotyledons. Trees resulting from the parsimony analysis are similar to those generated by earlier single or multiple gene analyses, but their strict consensus tree provides much better resolution of relationships among major clades. We find that Acorus (Acorales) is a sister group to the rest of the monocots, which receives 100% bootstrap support. A clade comprising Alismatales is diverged as the next branch, followed successively by Petrosaviaceae, the Dioscoreales–Pandanales clade, Liliales, Asparagales and commelinoids. All of these clades are strongly supported (with more than 90% bootstrap support). The sister-group relationship is also strongly supported between Alismatales and the remaining monocots (except for Acorus) (100%), between Petrosaviaceae and the remaining monocots (except for Acorus and Alismatales) (100%), between the clade comprising Dioscoreales and Pandanales and the clade comprising Liliales, Asparagales and commelinoids (87%), and between Liliales and the Asparagales–commelinoids clade (89%). Only the sister-group relationship between Asparagales and commelinoids is weakly supported (68%). Results also support the inclusion of Petrosaviaceae in its own order Petrosaviales, Nartheciaceae in Dioscoreales and Hanguanaceae in Commelinales.

Keywords

Hanguanaceae matK Molecular phylogeny Monocotyledons Petrosaviaceae rbcL 

Notes

Acknowledgements

We express our sincere gratitude to Hiroshi Tobe for helpful comments and critical reading of the manuscript, to Wendy B. Zomlefer and Hidetoshi Nagamasu for supplying us with plant materials used in this study, to Pu Fading of Chengdu Institute of Biology, Academia Sinica, China and staff members of Wawushan National Forest Park, China and Teshio Experimental Forest, Hokkaido University, Japan for their assistance in various ways in fieldwork, and to Moritoshi Iino for allowing us to use his ABI auto-sequencer freely. The study was supported in part by Grants-in-Aid for Scientific Research (11640701 and 14405012) from the Ministry of Education, Science and Culture, Japan.

Supplementary material

Appendix Insertion/deletion of DNA fragments into/from the matK gene region postulated based on the gaps recognized in comparisons between aligned DNA sequences

supp.pdf (98 kb)
(PDF 42 KB)

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Copyright information

© The Botanical Society of Japan and Springer-Verlag  2004

Authors and Affiliations

  • Minoru N. Tamura
    • 1
  • Jun Yamashita
    • 1
  • Shizuka Fuse
    • 1
    • 2
  • Masatake Haraguchi
    • 1
  1. 1.Botanical Gardens, Graduate School of ScienceOsaka City UniversityOsaka 576-0004Japan
  2. 2.Museum of Nature and Human ActivitiesHyogoJapan

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