Journal of Plant Research

, Volume 116, Issue 6, pp 455–460 | Cite as

Expression of a bacterial aroA mutant, aroA-M1, encoding 5-enolpyruvylshikimate-3-phosphate synthase for the production of glyphosate-resistant tobacco plants

Original Article

Abstract

Glyphosate is a non-selective broad-spectrum herbicide that inhibits 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS), a key enzyme in the aromatic amino acid biosynthetic pathway in microorganisms and plants. We have previously reported a strategy for engineering glyphosate-resistant class I EPSPS based on staggered-PCR technology. Selected mutant enzymes exhibited high Ki[glyphosate] and low Km[PEP] values compared to the parental enzymes from Escherichia coli (EcaroA) and Salmonella typhimurium (StaroA). One mutant, aroA-M1, was further engineered with a tobacco chloroplast leader sequence, and then placed in the binary vector pCAMBIA1300 for Agrobacterium-mediated gene transfer to tobacco (Nicotiana tabacum cv. Xanthi). Transgenic plants with increased resistance to glyphosate were generated.

Keywords

aroA mutant Bacterial EPSPS Glyphosate Protein engineering Transgenic tobacco 

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Copyright information

© The Botanical Society of Japan and Springer-Verlag  2004

Authors and Affiliations

  • He-Yong Wang
    • 1
  • Yun-Feng Li
    • 1
  • Long-Xu Xie
    • 1
  • Peilin Xu
    • 1
  1. 1.Biotechnology Research Center, The Key Laboratory of Gene Engineering of the Education MinistryZhongshan UniversityGuangzhouPeople's Republic of China

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