Journal of Plant Research

, Volume 116, Issue 4, pp 331–336 | Cite as

Physical mapping of rDNA sequences in four karyotypes of Ranunculus silerifolius (Ranunculaceae)

  • Chigusa Takahashi
Short Communication


The chromosomal locations of the 18S-5.8S-26S rDNA and 5S rDNA sequences were examined in four cytotypes of Ranunculus silerifolius (the Matsuyama, Mugi, Otaru, and Karatsu types) using fluorescence in situ hybridization (FISH). Using the 18S-5.8S-26S rDNA probe, one pair of probe hybridization sites was detected by FISH in the interstitial region corresponding to the secondary constriction on the short arm of a satellite chromosome (chromosome pair 6) in all four karyotypes. FISH using 5S rDNA identified one pair of sites. The 5S rDNA locus was on different chromosomes in the four karyotypes: in the interstitial region of the short arm of the largest metacentric chromosome (chromosome pair 1) in the Matsuyama type, in the interstitial region of the short arm of the subtelocentric chromosome (pair 2) in the Mugi and Otaru types, and in the interstitial region of the short arm of the metacentric chromosome (pair 2) in the Karatsu type. This physical mapping of the 5S rDNA provides valuable information about karyotype evolution in R. silerifolius. Possible mechanisms of chromosome evolution are discussed.


Chromosome evolution Fluorescence in situ hybridization Ranunculus silerifolius 18S-5.8S-26S rDNA 5S rDNA 



I thank Dr. H. Fujishima for his helpful suggestions, comments and critical reading of the manuscript.


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Copyright information

© The Botanical Society of Japan and Springer-Verlag  2003

Authors and Affiliations

  1. 1.Faculty of Education and Regional SciencesTottori UniversityTottori 680–8551Japan

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