Journal of Plant Research

, Volume 116, Issue 4, pp 331–336 | Cite as

Physical mapping of rDNA sequences in four karyotypes of Ranunculus silerifolius (Ranunculaceae)

Short Communication

Abstract

The chromosomal locations of the 18S-5.8S-26S rDNA and 5S rDNA sequences were examined in four cytotypes of Ranunculus silerifolius (the Matsuyama, Mugi, Otaru, and Karatsu types) using fluorescence in situ hybridization (FISH). Using the 18S-5.8S-26S rDNA probe, one pair of probe hybridization sites was detected by FISH in the interstitial region corresponding to the secondary constriction on the short arm of a satellite chromosome (chromosome pair 6) in all four karyotypes. FISH using 5S rDNA identified one pair of sites. The 5S rDNA locus was on different chromosomes in the four karyotypes: in the interstitial region of the short arm of the largest metacentric chromosome (chromosome pair 1) in the Matsuyama type, in the interstitial region of the short arm of the subtelocentric chromosome (pair 2) in the Mugi and Otaru types, and in the interstitial region of the short arm of the metacentric chromosome (pair 2) in the Karatsu type. This physical mapping of the 5S rDNA provides valuable information about karyotype evolution in R. silerifolius. Possible mechanisms of chromosome evolution are discussed.

Keywords

Chromosome evolution Fluorescence in situ hybridization Ranunculus silerifolius 18S-5.8S-26S rDNA 5S rDNA 

References

  1. Fujishima H (1983) Karyological and biogeographical studies of Ranunculus silerifolius Lév. from Tottori Prefecture. J Fac Educ Tottori Univ Nat Sci 32:31–37Google Scholar
  2. Fujishima H (1985a) Cytological studies on two varieties of Ranunculus silerifolius Lév. in Japan. Jpn J Genet 60:215–224Google Scholar
  3. Fujishima H (1985b) Geographical distribution of cytotypes in Ranunculus silerifolius Lév. in Japan. J Fac Educ Tottori Univ Nat Sci 34:23–40Google Scholar
  4. Fujishima H (1985c) Karyological studies on two cytotypes in Ranunculus silerifolius Lév. J Fac Educ Tottori Univ Nat Sci 34:97–116Google Scholar
  5. Fujishima H (1988) Cytogenetical studies on the karyotype differentiation in Ranunculus silerifolius Léveillé. J Fac Educ Tottori Univ Nat Sci 37:33–90Google Scholar
  6. Fujishima H, Kurita M (1974) Chromosome studies in Ranunculaceae, XXVI. Variation in karyotype of Ranunculus ternatus var. Glaber. Mem Ehime Univ Sci Ser B 7:4–10Google Scholar
  7. Gerlach WL, Bedbrook JR (1979) Cloning and characterization of ribosomal RNA genes from wheat and barley. Nucleic Acids Res 7:1869–1885PubMedGoogle Scholar
  8. Hizume M (1993) Chromosomal localization of 5S rRNA genes in Vicia faba and Crepis capillaries. Cytologia 58:417–421Google Scholar
  9. Levan A, Fredga K, Sandberg AA (1964) Nomenclature for centromeric position on chromosomes. Hereditas 52:201–220Google Scholar
  10. Meinkoth J, Wahl G (1984) Hybridization of nucleic acid immobilized on solid supports. Anal Biochem 138:267–284PubMedGoogle Scholar
  11. Murray MG, Thompson WF (1980) Rapid isolation of high molecular weight plant DNA. Nucleic Acids Res 8:4321–4325PubMedGoogle Scholar
  12. Ohwi J (1984) Flora of Japan. Smithsonian Institution Washington, D.C.Google Scholar
  13. Okada H (1981) On sexual isolation caused by karyotype variations in Ranunculus silerifolius Lév (in Japanese). J Jpn Bot 56:41–49Google Scholar
  14. Okada H (1984) Polyphyletic allopolyploid origin of Ranunculus cantoniensis (4×) from R. silerifolius (2×) × R. chinensis (2×). Plant Syst Evol 148:89–102Google Scholar
  15. Okada H, Tamura M (1977) Chromosome variations in Ranunculus quelpaertensis and its allied species (in Japanese). J Jpn Bot 52:360–369Google Scholar
  16. Takahashi C, Leitch IJ, Ryan A, Bennett MD, Brandham PE (1997) The use of genomic in situ hybridization (GISH) to show transmission of recombinant chromosomes by a partially fertile bigeneric hybrid, Gasteria lutzii × Aloe aristata (Aloaceae), to its progeny. Chromosoma 105:342–348CrossRefPubMedGoogle Scholar

Copyright information

© The Botanical Society of Japan and Springer-Verlag  2003

Authors and Affiliations

  1. 1.Faculty of Education and Regional SciencesTottori UniversityTottori 680–8551Japan

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