Journal of Plant Research

, Volume 116, Issue 2, pp 165–167 | Cite as

Electrophoretic evidence for homoeologous chromosome pairing in the apogamous fern species Dryopteris nipponensis (Dryopteridaceae)

  • Hiroshi IshikawaEmail author
  • Motomi Ito
  • Yasuyuki Watano
  • Siro Kurita
Short Communication


Segregation of genotypes through homoeologous chromosome pairing in the apogamous species Dryopteris nipponensis was tested by electrophoretic analysis. Of 284 progeny examined (250 gametophytes and 34 sporophytes), from the parental sporophyte with the Pgi-2 genotype abc, five showed different genotypes from that of the parent (three aac, one bbc and one bcc). This is the first evidence for genetic segregation in the progeny of apogamous fern species.


Allozyme Apogamy Dryopteris Genotype segregation Homoeologous chromosome pairing 


  1. Chapman RH, Klekowski EJ, Selander RK (1979) Homoeologous heterozygosity and recombination in the fern Pteridium aquilinum. Science 204:1207–1209Google Scholar
  2. Gastony GJ, Gottlieb LD (1982) Evidence for genetic heterozygosity in a homosporous fern. Am J Bot 69:634–637Google Scholar
  3. Gastony GJ, Gottlieb LD (1985) Genetic variation in the homosporous fern Pellaea andromedifolia. Am J Bot 72:257–267Google Scholar
  4. Hirabayashi H (1967) Chromosome numbers in Japanese species of Dryopteris (2). J Jap Bot 42:44–48Google Scholar
  5. Hirabayashi H (1974) Cytogeographic studies on Dryopteris of Japan. Hara Shobo, TokyoGoogle Scholar
  6. Ishikawa H, Ito M, Watano Y, Kurita S (2003) Extensive electrophoretic variation of the apogamous fern species, Dryopteris nipponensis (Dryopteridaceae). Acta Phytotax Geobot 54 (in press)Google Scholar
  7. Klekowski EJ (1973) Sexual and subsexual systems in homosporous pteridophytes: a new hypothesis. Am J Bot 60:535–544Google Scholar
  8. Klekowski EJ, Hickok LG (1974) Non homologous chromosome pairing in the fern Ceratopteris. Amer J Bot 61:422–432Google Scholar
  9. Kurita S (1966) Chromosome numbers of some Japanese ferns (6). J Jap Bot 41:173–180Google Scholar
  10. Kurita S (1967) Chromosome numbers of some Japanese ferns (7). Ann Rep Foreign Stud Coll Chiba Univ 2:57–61Google Scholar
  11. Lin S-J, Kato M, Iwatsuki K (1992) Diploid and triploid offspring of triploid agamosporous fern Dryopteris pacifica. Bot Mag Tokyo 105:443–452Google Scholar
  12. Lin S-J, Kato M, Iwatsuki K (1995) Electrophoretic variation of the apogamous Dryopteris varia group (Dryopteridaceae). J Plant Res 108:451–456Google Scholar
  13. Manton I (1950) Problems of cytology and evolution in the Pteridophyta. University Press, CambridgeGoogle Scholar
  14. Soltis DE, Haufler CH, Darrow CD, Gastony JG (1983) Starch gel electrophoresis of ferns: acomparison of grinding buffers, gel and electrode buffers, and staining schedules. Am Fern J 73:9–27Google Scholar
  15. Suzuki T, Iwatsuki K (1990) Genetic variation in agamosporous fern Pteris cretica L. in Japan. Heredity 65:221–227Google Scholar
  16. Takamiya M, Ohta N, Yatabe Y, Murakami N (2001) Cytological, morphological, genetic and molecular phylogenetic studies on intraspecific differentiations within Diplazium doederleinii (Woodsiaceae: Pteridophyta). Int J Plant Sci 162:625–636CrossRefGoogle Scholar
  17. Walker TG (1962) Cytology and evolution in the fern genus Pteris L. Evolution 16:27–43Google Scholar
  18. Watano Y, Iwatsuki K (1988) Genetic variation in the 'Japanese apogamous form' of the fern Asplenium unilaterale Lam. Bot Mag Tokyo 101:213–222Google Scholar
  19. Watano Y, Masuyama S (1991) Inbreeding in natural populations of the annual polyploid fern Ceratopteris thalictroides (Parkeriaceae). Syst Bot 16:705–714Google Scholar

Copyright information

© The Botanical Society of Japan and Springer-Verlag  2003

Authors and Affiliations

  • Hiroshi Ishikawa
    • 1
    Email author
  • Motomi Ito
    • 2
  • Yasuyuki Watano
    • 3
  • Siro Kurita
    • 3
  1. 1.Graduate School of Science and TechnologyChiba UniversityChiba 263-8522Japan
  2. 2.Graduate School of Arts and SciencesUniversity of TokyoTokyoJapan
  3. 3.Faculty of ScienceChiba UniversityChibaJapan

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