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Clinical and Experimental Medicine

, Volume 2, Issue 2, pp 105–108 | Cite as

Single-tube genotyping of MBL-2 polymorphisms using melting temperature analysis

  • U. Hladnik
  • L. Braida
  • M. Boniotto
  • D. Pirulli
  • F. Gerin
  • A. Amoroso
  • S. Crovella
SHORT COMMUNICATION

Abstract

Recently several authors correlated MBL-2 gene polymorphisms with different pathologies and there is a growing interest for MBL-2 genotyping in a large number of individuals. We have developed a single-tube, rapid, economic, and fully automated melting temperature analysis screening method, based on ABI 7700 Sequence Detection System technology and SYBR Green I chemistry, for the detection of three polymorphisms (exon 1, codons 52, 54, 57) in the MBL-2 gene. We also developed an electronic sheet for the automatic calling of different genotypes, based on the analysis of the first derivative of ABI 7700 raw data.

Key wordsMBL-2 Polymorphisms Melting temperature analysis Automatic allele calling 

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Copyright information

© Springer-Verlag Italia 2002

Authors and Affiliations

  • U. Hladnik
    • 1
  • L. Braida
    • 1
  • M. Boniotto
    • 1
  • D. Pirulli
    • 1
  • F. Gerin
    • 1
  • A. Amoroso
    • 1
  • S. Crovella
    • 1
  1. 1.Dipartimento di Scienze della Riproduzione e dello Sviluppo, Sezione di Genetica, Università di Trieste, Via dell'Istria 65/1, I-34100 Trieste, Italy e-mail: crovella@burlo.trieste.it Tel.: +39-040-3785538 Fax: +39-040-3785540IT

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