TNF-R2 in tumor microenvironment as prognostic factor in epithelial ovarian cancer
The aims of the study were to compare the levels of tumor necrosis factor alpha (TNF-α) and its soluble type I (sTNF-R1) and type II (sTNF-R2) receptors detected in intracystic liquid and serum from benign and malignant ovarian neoplasms and to relate them to prognostic factors in epithelial ovarian cancer. The patients were divided into benign ovarian neoplasms (n = 46) and malignant ovarian neoplasms (n = 17). The serum and intracystic samples were collected before and during surgery for ovarian cyst, respectively. The levels of TNF-α, sTNF-R1, and sTNF-R2 were measured using ELISA. Results were compared with the Mann–Whitney test. Concentration of sTNF-R2 in the intracystic samples collected from the malignant neoplasia was significantly higher than that of the benign neoplasias (p = 0.02). Higher intracystic levels of sTNF-R2 exhibited a significant association with tumor differentiation grades 2 and 3 (p = 0.0087). There was no statistical significance in relation to serum levels. Tumor microenvironment levels of sTNF-R2 may represent a factor of poor prognosis in epithelial ovarian cancer.
KeywordsTumor necrosis factor alpha Tumor necrosis factor receptor 1 Tumor necrosis factor receptor 2 Intracystic fluid Ovarian neoplasm Prognostic factor
The authors wish to acknowledge the funding received from the CNPq, FUNEPU, and the FAPEMIG.
The funding sources financed the purchase of the ELISA kits and the necessary equipment for the storage of the samples and the realization of the experiments in the laboratory.
Compliance with ethical standards
Conflict of interest
The authors report no conflicts of interest.
The study was approved by the Research Ethics Committee (CEP) of UFTM, CAAE 30507414.5.0000.5154.
Consent was obtained from all patients involved in the study.
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