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Clinical and Experimental Medicine

, Volume 18, Issue 4, pp 547–554 | Cite as

TNF-R2 in tumor microenvironment as prognostic factor in epithelial ovarian cancer

  • Rosekeila Simões Nomelini
  • Luciano Eliziário Borges Júnior
  • Cid Almeida de Lima
  • Ana Flávia Carrijo Chiovato
  • Douglas Côbo Micheli
  • Beatriz Martins Tavares-Murta
  • Eddie Fernando Candido Murta
Original Article

Abstract

The aims of the study were to compare the levels of tumor necrosis factor alpha (TNF-α) and its soluble type I (sTNF-R1) and type II (sTNF-R2) receptors detected in intracystic liquid and serum from benign and malignant ovarian neoplasms and to relate them to prognostic factors in epithelial ovarian cancer. The patients were divided into benign ovarian neoplasms (n = 46) and malignant ovarian neoplasms (n = 17). The serum and intracystic samples were collected before and during surgery for ovarian cyst, respectively. The levels of TNF-α, sTNF-R1, and sTNF-R2 were measured using ELISA. Results were compared with the Mann–Whitney test. Concentration of sTNF-R2 in the intracystic samples collected from the malignant neoplasia was significantly higher than that of the benign neoplasias (p = 0.02). Higher intracystic levels of sTNF-R2 exhibited a significant association with tumor differentiation grades 2 and 3 (p = 0.0087). There was no statistical significance in relation to serum levels. Tumor microenvironment levels of sTNF-R2 may represent a factor of poor prognosis in epithelial ovarian cancer.

Keywords

Tumor necrosis factor alpha Tumor necrosis factor receptor 1 Tumor necrosis factor receptor 2 Intracystic fluid Ovarian neoplasm Prognostic factor 

Notes

Acknowledgements

The authors wish to acknowledge the funding received from the CNPq, FUNEPU, and the FAPEMIG.

Funding source

The funding sources financed the purchase of the ELISA kits and the necessary equipment for the storage of the samples and the realization of the experiments in the laboratory.

Compliance with ethical standards

Conflict of interest

The authors report no conflicts of interest.

Ethical approval

The study was approved by the Research Ethics Committee (CEP) of UFTM, CAAE 30507414.5.0000.5154.

Informed consent

Consent was obtained from all patients involved in the study.

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Copyright information

© Springer International Publishing AG, part of Springer Nature 2018

Authors and Affiliations

  • Rosekeila Simões Nomelini
    • 1
  • Luciano Eliziário Borges Júnior
    • 1
  • Cid Almeida de Lima
    • 1
  • Ana Flávia Carrijo Chiovato
    • 1
  • Douglas Côbo Micheli
    • 2
  • Beatriz Martins Tavares-Murta
    • 2
  • Eddie Fernando Candido Murta
    • 1
  1. 1.Research Institute of Oncology (IPON)/Discipline of Gynecology and ObstetricsFederal University of Triângulo MineiroUberabaBrazil
  2. 2.Discipline of PharmacologyFederal University of Triângulo MineiroUberabaBrazil

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