Cytoplasmic expression of Twist1, an EMT-related transcription factor, is associated with higher grades renal cell carcinomas and worse progression-free survival in clear cell renal cell carcinoma

  • Arezoo Rasti
  • Zahra Madjd
  • Maryam Abolhasani
  • Mitra Mehrazma
  • Leila Janani
  • Leili Saeednejad Zanjani
  • Mojgan Asgari
Original Article

Abstract

Twist1 is a key transcription factor, which confers tumor cells with cancer stem cell (CSC)-like characteristics and enhances epithelial–mesenchymal transition in pathological conditions including tumor malignancy and metastasis. This study aimed to evaluate the expression patterns and clinical significance of Twist1 in renal cell carcinoma (RCC). The cytoplasmic and nuclear expression of Twist1 were examined in 252 well-defined renal tumor tissues, including 173 (68.7%) clear cell renal cell carcinomas (ccRCC), 45 (17.9%) papillary renal cell carcinomas (pRCC) and 34 (13.5%) chromophobe renal cell carcinoma, by immunohistochemistry on a tissue microarray. The association between expression of this marker and clinicopathologic parameters and survival outcomes were then analyzed. Twist1 was mainly localized to the cytoplasm of tumor cells (98.8%). Increased cytoplasmic expression of Twist1 was associated with higher grade tumors (P = 0.045), renal vein invasion (P = 0.031) and microvascular invasion (P = 0.044) in RCC. It was positively correlated with higher grade tumors (P = 0.026), shorter progression-free survival time (P = 0.027) in patients with ccRCC, and also with higher stage in pRCC patients (P = 0.036). Significantly higher cytoplasmic expression levels of Twist1 were found in ccRCC and pRCC subtypes, due to their more aggressive tumor behavior. Increased cytoplasmic expression of Twist1 had a critical role in worse prognosis in ccRCC. These findings suggest that cytoplasmic, rather than nuclear expression of Twist1 can be considered as a prognostic and therapeutic marker for targeted therapy of RCC, especially for ccRCC patients.

Keywords

Twist1 Epithelial–mesenchymal transition (EMT) Renal cell carcinoma (RCC) Prognosis Tissue microarray (TMA) 

Notes

Acknowledgements

The authors are grateful to Elham Kalantari for her excellent technical assistance.

Funding

This research was supported by a Grant of the Iran University of Medical Sciences (Grant #25166).

Compliance with ethical standards

Conflict of interest

All authors declare that they have no conflict of interest.

Ethical approval

All procedures performed in studies involving human participants were in accordance with the ethical standards of the institutional and/or national research committee and with the 1964 Declaration of Helsinki and its later amendments or comparable ethical standards. This research study was approved by the Iran University of Medical Sciences Research Ethics Committee.

Informed consent

Informed consent was obtained from all individual participants included in the study.

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© Springer International Publishing AG, part of Springer Nature 2017

Authors and Affiliations

  1. 1.Oncopathology Research CentreIran University of Medical Sciences (IUMS)TehranIran
  2. 2.Department of Molecular Medicine, Faculty of Advanced Technologies in MedicineIran University of Medical SciencesTehranIran
  3. 3.Hasheminejad Kidney CenterIran University of Medical Sciences, (IUMS)TehranIran
  4. 4.Department of Biostatistics, School of Public HealthIran University of Medical Sciences (IUMS)TehranIran

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