Clinical and Experimental Medicine

, Volume 15, Issue 1, pp 41–45 | Cite as

Novel therapy for insulin-dependent diabetes mellitus: infusion of in vitro-generated insulin-secreting cells

  • S. D. DaveEmail author
  • A. V. Vanikar
  • H. L. Trivedi
  • U. G. Thakkar
  • S. C. Gopal
  • T. Chandra
Original Article


Insulin-dependent diabetes mellitus (IDDM) is a metabolic disease usually resulting from autoimmune-mediated β-cell destruction requiring lifetime exogenous insulin replacement. Mesenchymal stem cells (MSC) hold promising therapy. We present our experience of treating IDDM with co-infusion of in vitro autologous adipose tissue-derived MSC-differentiated insulin-secreting cells (ISC) with hematopoietic stem cells (HSC). This was an Institutional Review Board approved prospective non-randomized open-labeled clinical trial after informed consent from ten patients. ISC were differentiated from autologous adipose tissue-derived MSC and were infused with bone marrow-derived HSC in portal, thymic circulation by mini-laparotomy and in subcutaneous circulation. Patients were monitored for blood sugar levels, serum C-peptide levels, glycosylated hemoglobin (Hb1Ac) and glutamic acid decarboxylase (GAD) antibodies. Insulin administration was made on sliding scale with an objective of maintaining FBS < 150 mg/dL and PPBS around 200 mg/dL. Mean 3.34 mL cell inoculums with 5.25 × 104 cells/μL were infused. No untoward effects were observed. Over a mean follow-up of 31.71 months, mean serum C-peptide of 0.22 ng/mL before infusion had sustained rise of 0.92 ng/mL with decreased exogenous insulin requirement from 63.9 international units (IU)/day to 38.6 IU/day. Improvement in mean Hb1Ac was observed from 10.99 to 6.72 %. Mean GAD antibodies were positive in all patients with mean of 331.10 IU/mL, which decreased to mean of 123 IU/mL. Co-infusion of autologous ISC with HSC represents a viable novel therapeutic option for IDDM.


Insulin-secreting cells Adipose tissue Mesenchymal stem cells Hematopoietic stem cells Insulin-dependent diabetes mellitus 



Bone marrow


Cultured bone marrow


Diabetic ketoacidosis


Fasting blood sugar levels


Glutamic acid decarboxylase


Glycosylated hemoglobin


Hematopoietic stem cells


Insulin-secreting cells


Mesenchymal stem cells


Postprandial blood sugar level


Insulin-dependent diabetes mellitus



The authors thank the staff and technicians of IKDRC-ITS, India, for all the technical help. The authors also thank Shobhana Sengunthar for providing statistical analysis.

Conflict of interest



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Copyright information

© Springer-Verlag Italia 2013

Authors and Affiliations

  • S. D. Dave
    • 1
    Email author
  • A. V. Vanikar
    • 1
  • H. L. Trivedi
    • 2
    • 3
  • U. G. Thakkar
    • 3
  • S. C. Gopal
    • 4
  • T. Chandra
    • 5
  1. 1.Stem Cell Lab, Transplantation Biology Research Centre, Department of Pathology, Laboratory Medicine, Transfusion Services and ImmunohematologyG. R. Doshi and K. M. Mehta Institute of Kidney Diseases and Research Centre (IKDRC)-Dr. H.L. Trivedi Institute of Transplantation Sciences (ITS)AhmedabadIndia
  2. 2.Department of Nephrology and Transplantation MedicineIKDRC-ITSAhmedabadIndia
  3. 3.Department of Stem Cell Therapy and Regenerative MedicineIKDRC-ITSAhmedabadIndia
  4. 4.Department of SurgeryBanaras Hindu UniversityVaranasiIndia
  5. 5.Department of Transfusion MedicineKing George Medical UniversityLucknowIndia

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