Clinical and Experimental Medicine

, Volume 14, Issue 4, pp 431–437

Impact of methotrexate on oxidative stress and apoptosis markers in psoriatic patients

  • Tamilselvi Elango
  • Haripriya Dayalan
  • Pushpa Gnanaraj
  • Hemamalini Malligarjunan
  • Swapna Subramanian
Original Article

DOI: 10.1007/s10238-013-0252-7

Cite this article as:
Elango, T., Dayalan, H., Gnanaraj, P. et al. Clin Exp Med (2014) 14: 431. doi:10.1007/s10238-013-0252-7

Abstract

Methotrexate (MTX), a cytotoxic chemotherapeutic agent, is considered an effective drug in the treatment of psoriasis. The aim of this study was to find out whether the effect of MTX treatment in psoriasis is due to oxidative stress-induced apoptosis. Psoriasis vulgaris patients (58 in number) were recruited for this study. Healthy volunteers (45 in number) served as control. Samples of psoriatic patients were collected and analyzed for total reactive oxygen species (ROS), malondialdehyde (MDA) levels, nitrite, nitrate levels and the activities of antioxidants like superoxide dismutase (SOD), catalase (CAT) and total antioxidant status (TAS) and also the protein expression of caspase-3, before (Day 0) and after (at the end of 6 and 12 weeks) MTX treatment. Our results show a significant increase in tissue ROS and plasma MDA after MTX treatment when compared with before MTX treatment in psoriasis patients (p < 0.001). The levels of serum nitrite and nitrate were decreased significantly after MTX treatment (p < 0.001). The activities of plasma SOD, TAS and serum CAT levels were decreased, but not significantly after 12 weeks of treatment. The expression of caspase-3 was increased after MTX treatment. In conclusion, MTX induce apoptosis through oxidative stress by reducing NO and increasing caspase-3 levels. MTX-induced apoptosis may account for the beneficial effect of MTX treatment in psoriasis patients, which is characterized by acanthosis.

Keywords

Reactive oxygen species Methotrexate Apoptosis Psoriasis 

Copyright information

© Springer-Verlag Italia 2013

Authors and Affiliations

  • Tamilselvi Elango
    • 1
  • Haripriya Dayalan
    • 1
  • Pushpa Gnanaraj
    • 2
  • Hemamalini Malligarjunan
    • 1
  • Swapna Subramanian
    • 1
  1. 1.Department of Medical ResearchSRM Medical College, Hospital and Research CentreKattankulathurIndia
  2. 2.Department of DermatologySRM Medical College, Hospital and Research CentreKattankulathurIndia

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