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Clinical and Experimental Medicine

, Volume 13, Issue 3, pp 221–224 | Cite as

hTERT and TP53 deregulation in intestinal-type gastric carcinogenesis in non-human primates

  • Mariana Ferreira LealEmail author
  • Danielle Queiroz Calcagno
  • André Salim Khayat
  • Tanielly Cristina Raiol Silva
  • José Augusto Pereira Carneiro Muniz
  • Paulo Pimentel Assumpção
  • Marília de Arruda Cardoso Smith
  • Rommel Rodríguez Burbano
Letter to the Editor

Abstract

Despite the high incidence, the molecular events involved in intestinal-type gastric carcinogenesis remains unclear. We previously established an intestinal-type gastric carcinogenesis model in Cebus apella, a New World monkey. In the present study, we evaluated hTERT and TP53 mRNA expression, as well as their protein immunoreactivity, in normal mucosa, non-atrophic gastritis, atrophic gastritis, intestinal metaplasia, and intestinal-type gastric cancer samples of non-human primates treated with N-methyl-nitrosourea. In addition, we evaluated the number of TP53 copies in these samples. Although hTERT immunoreactivity was only detected in gastric cancer, a continuous increase of hTERT mRNA expression was observed from non-atrophic gastritis to gastric tumors. No sample presented p53 immunoreactivity. However, we also observed a continuous decrease of TP53 mRNA expression during the sequential steps of gastric carcinogenesis. Moreover, loss of TP53 copies was observed in intestinal metaplasia and gastric cancer samples. Our study highlights that hTERT and TP53 have a key role in intestinal-type gastric cancer initiation.

Keywords

hTERT TP53 Gastric carcinogenesis Non-human primates Precancerous lesions Animal model 

Notes

Acknowledgments

This study was supported by Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq; MACS and RRB) and Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP; MFL and DQC) as grants and fellowship awards.

Conflict of interest

All authors declare that they have no conflicts of interest.

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Copyright information

© Springer-Verlag 2012

Authors and Affiliations

  • Mariana Ferreira Leal
    • 1
    Email author
  • Danielle Queiroz Calcagno
    • 1
  • André Salim Khayat
    • 2
  • Tanielly Cristina Raiol Silva
    • 2
  • José Augusto Pereira Carneiro Muniz
    • 3
  • Paulo Pimentel Assumpção
    • 4
  • Marília de Arruda Cardoso Smith
    • 1
  • Rommel Rodríguez Burbano
    • 2
  1. 1.Disciplina de Genética, Departamento de Morfologia e GenéticaUniversidade Federal de São PauloSão PauloBrazil
  2. 2.Laboratório de Citogenética Humana, Instituto de Ciências BiológicasUniversidade Federal do ParáBelémBrazil
  3. 3.Centro Nacional de PrimatasMinistério da SaúdeAnanindeuaBrazil
  4. 4.Unidade de Alta Complexidade em OncologiaHospital Universitário João de Barros Barreto, Universidade Federal do ParáBelémBrazil

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