Clinical and Experimental Medicine

, Volume 10, Issue 1, pp 7–13 | Cite as

Effects of exposure to hyperbaric oxygen on oxidative stress in rats with type II collagen-induced arthritis

  • F. Nagatomo
  • N. Gu
  • H. Fujino
  • T. Okiura
  • F. Morimatsu
  • I. Takeda
  • A. IshiharaEmail author
Original Article


Arthritis was induced in 9-week-old female Dark Agouti rats by injecting type II collagen. Serum levels of the derivatives of reactive oxygen metabolites (dROMs), which are oxidative stress markers, and C-reactive protein (CRP) in arthritic rats that were exposed to a pressure of 1.25 atmospheres absolute and an oxygen concentration of 36% for 3 weeks (arthritis + HBO group) were compared to those of control rats (control group) and arthritic rats that were not exposed to hyperbaric oxygen (arthritis group). The body weights of the arthritis and arthritis + HBO groups were lower than that of the control group, whereas no difference in the body weight was observed between the arthritis and arthritis + HBO groups. The serum levels of dROMs and CRP in the arthritis group were higher than those in the control and arthritis + HBO groups. No difference in the serum level of CRP was observed between the control and arthritis + HBO groups. These results indicate that the conditions of hyperbaric oxygen exposure used in this study are effective for reducing the levels of reactive oxygen species, which are overproduced during arthritis.


C-reactive protein Derivatives of reactive oxygen metabolites Hyperbaric oxygen exposure Rat Reactive oxygen species Type II collagen-induced arthritis 


Conflict of interest statement

The authors declare that they have no conflict of interest related to the publication of this manuscript.


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Copyright information

© Springer-Verlag 2009

Authors and Affiliations

  • F. Nagatomo
    • 1
  • N. Gu
    • 1
  • H. Fujino
    • 2
  • T. Okiura
    • 3
  • F. Morimatsu
    • 3
  • I. Takeda
    • 4
  • A. Ishihara
    • 1
    Email author
  1. 1.Laboratory of Neurochemistry, Graduate School of Human and Environmental StudiesKyoto UniversityKyotoJapan
  2. 2.Division of Rehabilitation Sciences, Graduate School of Health SciencesKobe UniversityKobeJapan
  3. 3.Research and Development CenterNippon Meat Packers Inc.TsukubaJapan
  4. 4.Department of Physical Therapy, Faculty of Health Care ScienceHimeji Dokkyo UniversityHimejiJapan

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