Clinical and Experimental Medicine

, Volume 8, Issue 2, pp 87–91

Comparison of free plasma metanephrines enzyme immunoassay with 131I-MIBG scan in diagnosis of pheochromocytoma

  • Yun-Chao Gao
  • Han-Kui Lu
  • Quan-Yong Luo
  • Li-Bo Chen
  • Ying Ding
  • Rui-Sen Zhu
Original

DOI: 10.1007/s10238-008-0162-2

Cite this article as:
Gao, YC., Lu, HK., Luo, QY. et al. Clin. Exper.Med. (2008) 8: 87. doi:10.1007/s10238-008-0162-2

Abstract

Measurement of free plasma metanephrines (metanephrine and normetanephrine), usually performed by high-performance liquid chromatography with electrochemical detection (HPLC-ECD), has been recommended as the single biochemical test of choice for the diagnosis of pheochromocytoma. Alternatively, a widely available, simple means to measure these biomarkers with enzyme immunoassay (EIA) needs to be studied. The aim of this study was to investigate the diagnostic efficacy of such a method in comparison with 131I-metaiodobenzylguanidine (MIBG) whole body scan (WBS) in patients with pheochromocytoma. We enrolled patients undergoing 131I-MIBG WBS due to clinical findings suggestive of pheochromocytoma (n = 45), and patients with primary hypertension (n = 36). All subjects had blood tests for free plasma metanephrine (MN) and normetanephrine (NM) with a commercially available EIA kit. WBS was positive in 30 pheochromocytoma patients and negative in 15 refuted ones, with 100% accuracy. The sensitivity, specificity and accuracy of MN and NM in combination (either or both positive) were 96.7%, 86.3% and 90.1%, showing comparable diagnostic performance both to 131I-MIBG WBS (all p > 0.1), and also to the same markers measured with HPLC-ECD reported in the literature. These results showed that the EIA method may be eligible as an alternative to HPLC-ECD for plasma metanephrine determination in the identification of pheochromocytoma.

Keywords

Metanephrine Normetanephrine Pheochromocytoma Enzyme immunoassay 131I-metaiodobenzylguanidine scintigraphy 

Copyright information

© Springer-Verlag Italia 2008

Authors and Affiliations

  • Yun-Chao Gao
    • 1
  • Han-Kui Lu
    • 1
  • Quan-Yong Luo
    • 1
  • Li-Bo Chen
    • 1
  • Ying Ding
    • 1
  • Rui-Sen Zhu
    • 1
  1. 1.The Immunoassay Laboratory, Department of Nuclear MedicineShanghai Sixth People’s Hospital, Shanghai Jiao Tong UniversityShanghaiChina

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