Mutation of the p53 tumour suppressor gene and overexpression of its protein in 62 Japanese non-Hodgkin’s lymphomas

  • H. Kamata
  • S. Mitani
  • M. Fujiwara
  • N. Aoki
  • S. Okada
  • S. Mori
Original

Abstract

To clarify whether p53 mutation could be involved in the pathogenesis of various subtypes of lymphoma, we investigated 62 Japanese cases of non-Hodgkin’s lymphomas (NHLs) for p53 gene mutations and their relationship with the expression of p53 protein. Mutations in exons 5–9 of the p53 gene were screened for using the non-isotopic RNase cleavage assay (NIRCA) and confirmed by direct sequencing, followed by immunohistochemical analysis for p53 protein. Missense and/or nonsense mutations of p53 were detected in 3 (10.7%) of 28 diffuse large B-cell lymphomas (DLBLs) and 2 (15.4%) of 13 T-cell NHLs (15.4%). A single missense mutation at codon 157 (Val to Phe) in exon 5 and at codon 273 (Arg to Pro) in exon 8 was found respectively in 2 DLBLs and in one peripheral T-cell lymphoma (unspecified). In these 3 cases harbouring a missense mutation, overexpression of p53 protein was observed in more than 80% of tumour cells. Double transversion mutations comprising of a missense mutation at codon 167 (Gln to His) in exon 5 and a nonsense mutation at codon 183 (Ser to stop codon) in exon 5 were detected in one DLBL that had apparently transformed from follicular lymphoma and in one advanced adult T-cell lymphoma (ATL). In these two cases harbouring p53 nonsense mutation, no cells positive for p53 protein immunostaining were detected, as well as lymphomas without p53 mutation.

Key words

p53 Non-Hodgkin’s lymphoma Non-isotopic RNase cleavage assay 

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Copyright information

© Springer-Verlag Italia 2007

Authors and Affiliations

  • H. Kamata
    • 1
  • S. Mitani
    • 2
  • M. Fujiwara
    • 3
  • N. Aoki
    • 4
  • S. Okada
    • 5
  • S. Mori
    • 4
  1. 1.Laboratory of Veterinary MicrobiologyCollege of Bioresource Sciences Nihon UniversityKanagawaJapan
  2. 2.Center for Professional EducationKanagawa Prefectural University of Human ServiceKanagawaJapan
  3. 3.Division of PathologyThe Japanese Red Cross Medical CenterTokyoJapan
  4. 4.Department of PathologyTeikyo University School of MedicineTokyoJapan
  5. 5.Department of PathologyAgeo Medical LaboratorySaitamaJapan

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