Modelling apical columnar epithelium mechanics from circumferential contractile fibres
Simple columnar epithelia are formed by individual epithelial cells connecting together to form single cell high sheets. They are a main component of many important body tissues and are heavily involved in both normal and cancerous cell activities. Prior experimental observations have identified a series of contractile fibres around the circumference of a cross section located in the upper (apical) region of each cell. While other potential mechanisms have been identified in both the experimental and theoretical literature, these circumferential fibres are considered to be the most likely mechanism controlling movement of this cross section. Here, we investigated the impact of circumferential contractile fibres on movement of the cross section by creating an alternate model where movement is driven from circumferential contractile fibres, without any other potential mechanisms. In this model, we utilised a circumferential contractile fibre representation based on investigations into the movement of contractile fibres as an individual system, treated circumferential fibres as a series of units, and matched our model simulation to experimental geometries. By testing against laser ablation datasets sourced from existing literature, we found that circumferential fibres can reproduce the majority of cross-sectional movements. We also investigated model predictions related to various aspects of cross-sectional movement, providing insights into epithelium mechanics and demonstrating the usefulness of our modelling approach.
KeywordsColumnar epithelium Laser ablation Mechanistic modelling Vertex model Computational biomechanics Drosophila
This research was supported by the Victorian Life Sciences Computation Initiative (VLSCI) Grant UOM0012 on its Peak Computing Facility at the University of Melbourne, an initiative of the Victorian Government. We would also like to acknowledge the statistical support of the Statistical Consulting Centre at the University of Melbourne, specifically Rachel Sore.
Compliance with ethical standards
This study was funded by the Australian Government Department of Education and Training Australian Postgraduate Award.
Conflict of interest
The authors declare that they have no conflict of interest.
- Bambardekar K, Clment R, Blanc O, Chards C, Lenne PF (2015) Direct laser manipulation reveals the mechanics of cell contacts in vivo. In: Proceedings of the National Academy of Sciences, p 201418732Google Scholar
- Collinet C, Rauzi M, Lenne PF, Lecuit T (2015) Local and tissue-scale forces drive oriented junction growth during tissue extension. Nature cell biology Paper not direclty related to work I am doing currently, but very interestingGoogle Scholar
- Deguchi S, Ohashi T, Sato M (2005) Evaluation of tension in actin bundle of endothelial cells based on preexisting strain and tensile properties measurements. Mol Cell Biomech Online 2(3):125Google Scholar
- Geiger B, Dutton AH, Tokuyasu K, Singer S (1981) Immunoelectron microscope studies of membrane-microfilament interactions: distributions of alpha-actinin, tropomyosin, and vinculin in intestinal epithelial brush border and chicken gizzard smooth muscle cells. J Cell Biol 91(3):614–628CrossRefGoogle Scholar
- Kaunas R, Hsu H, Deguchi S (2011) Sarcomeric model of stretch-induced stress fiber reorganization. Cell Health Cytoskelet 3:13–22Google Scholar
- Louveaux M, Julien JD, Mirabet V, Boudaoud A, Hamant O (2016) Cell division plane orientation based on tensile stress in arabidopsis thaliana. In: Proceedings of the National Academy of Sciences, p 201600677Google Scholar
- Matsui TS, Deguchi S, Sakamoto N, Ohashi T, Sato M (2009) A versatile micro-mechanical tester for actin stress fibers isolated from cells. Biorheology 46(5):401–15Google Scholar
- Wang C, Tammi M, Guo H, Tammi R (1996) Hyaluronan distribution in the normal epithelium of esophagus, stomach, and colon and their cancers. Am J Pathol 148(6):1861Google Scholar
- Weliky M, Oster G (1990) The mechanical basis of cell rearrangement. Development 109(2):373–386Google Scholar
- Weliky M, Minsuk S, Keller R, Oster G (1991) Notochord morphogenesis in xenopus laevis: simulation of cell behavior underlying tissue convergence and extension. Development 113(4):1231–1244Google Scholar
- Wu J, Dickinson RB, Lele TP (2012) Investigation of in vivo microtubule and stress fiber mechanics with laser ablation. Integr Biol 4(5):471–479Google Scholar
- Yonemura S, Itoh M, Nagafuchi A, Tsukita S (1995) Cell-to-cell adherens junction formation and actin filament organization: similarities and differences between non-polarized fibroblasts and polarized epithelial cells. J Cell Sci 108(1):127–142Google Scholar