Determination of hip-joint loading patterns of living and extinct mammals using an inverse Wolff’s law approach
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It is well known that bone adapts its microstructure in response to loading. Based on this form-follows-function relationship, we previously developed a reverse approach to derive joint loads from bone microstructure as acquired with micro-computed tomography. Here, we challenge this approach by calculating hip-joint loading patterns for human and dog, two species exhibiting different locomotion, and comparing them to in vivo measurements. As a proof of concept to use the approach also for extinct taxa, we applied it to a cave lion fossil bone. Calculations were in close agreement with in vivo measurements during walking for extant species, showing distinguished patterns for bipedalism and quadrupedalism. The cave lion calculations clearly revealed its quadrupedal locomotion and suggested a more diverse behaviour compared to the dog, which is in agreement with extant felids. This indicates that our novel approach is potentially useful for making inferences about locomotion in living as well as extinct mammals and to study evolutionary joint development.
KeywordsBone form–function relationship Hip-joint loading patterns Bone/fossil microstructure Locomotion Micro-computed tomography Micro-finite element modelling
We thank Reinier van Zelst and Steven D. van der Mije from Naturalis Biodiversity Center for providing a cave lion fossil, Claudia F. Wolschrijn from Utrecht University for providing a dog femur, Ralph Müller from ETH Zurich for support through the VPHOP WP5 group, Joost J.A. de Jong for helping scanning the cave lion fossil, and Joop P.W. van den Bergh for providing the XtremeCT facility at Maastricht University. Funding from the European Union for the osteoporotic virtual physiological human project (VPHOP FP7-ICT2008-223865) is gratefully acknowledged.
- Gross C (1992) Das Skelett des Höhlenlöwen (Panthera leo spelaea Goldfuss, 1810) aus Siegsdorf/Ldkr. Traunstein im Vergleich mit anderen Funden aus Deutschland und den Niederlanden. PhD Thesis, Tierärztliche Fakultät der Maximilians-Universität, MünchenGoogle Scholar
- Lambers FM, Schulte FA, Kuhn G, Webster DJ, Muller R (2011) Mouse tail vertebrae adapt to cyclic mechanical loading by increasing bone formation rate and decreasing bone resorption rate as shown by time-lapsed in vivo imaging of dynamic bone morphometry. Bone 49:1340–1350. doi: 10.1016/J.Bone.2011.08.035 CrossRefGoogle Scholar
- Page AE, Allan C, Jasty M, Harrigan TP, Bragdon CR, Harris WH (1993) Determination of loading parameters in the canine hip in vivo. J Biomech 26:571–579. doi: 10.1016/0021-9290(93)90018-A
- Schulte FA, Ruffoni D, Lambers FM, Christen D, Webster DJ, Kuhn G, Muller R (2013) Local mechanical stimuli regulate bone formation and resorption in mice at the tissue level. Plos One 8. doi: 10.1371/journal.pone.0062172
- Sugiyama T, Meakin LB, Browne WJ, Galea GL, Price JS, Lanyon LE (2012) Bones’ adaptive response to mechanical loading is essentially linear between the low strains associated with disuse and the high strains associated with the lamellar/woven bone transition. J Bone Miner Res 27:1784–1793. doi: 10.1002/Jbmr.1599 CrossRefGoogle Scholar
- Ulrich D, van Rietbergen B, Weinans H, Ruegsegger P (1998) Finite element analysis of trabecular bone structure: a comparison of image-based meshing techniques. J Biomech 31:1187–1192Google Scholar
- Walker EP, Nowak RM (1991) Walker’s mammals of the world, 5th edn. Johns Hopkins University Press, BaltimoreGoogle Scholar
- Wolff J (1892) Das Gesetz der Transformation der Knochen. Verlag von August Hirschwald, BerlinGoogle Scholar