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Biomechanics and Modeling in Mechanobiology

, Volume 11, Issue 7, pp 983–993 | Cite as

Mechanical behaviour of in-situ chondrocytes subjected to different loading rates: a finite element study

  • E. K. Moo
  • W. Herzog
  • S. K. Han
  • N. A. Abu Osman
  • B. Pingguan-Murphy
  • S. Federico
Original Paper

Abstract

Experimental findings indicate that in-situ chondrocytes die readily following impact loading, but remain essentially unaffected at low (non-impact) strain rates. This study was aimed at identifying possible causes for cell death in impact loading by quantifying chondrocyte mechanics when cartilage was subjected to a 5% nominal tissue strain at different strain rates. Multi-scale modelling techniques were used to simulate cartilage tissue and the corresponding chondrocytes residing in the tissue. Chondrocytes were modelled by accounting for the cell membrane, pericellular matrix and pericellular capsule. The results suggest that cell deformations, cell fluid pressures and fluid flow velocity through cells are highest at the highest (impact) strain rate, but they do not reach damaging levels. Tangential strain rates of the cell membrane were highest at the highest strain rate and were observed primarily in superficial tissue cells. Since cell death following impact loading occurs primarily in superficial zone cells, we speculate that cell death in impact loading is caused by the high tangential strain rates in the membrane of superficial zone cells causing membrane rupture and loss of cell content and integrity.

Keywords

Finite element modelling Impact loading Chondrocyte mechanics Cell membrane damage Osteoarthritis Cartilage mechano-biology 

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Copyright information

© Springer-Verlag 2012

Authors and Affiliations

  • E. K. Moo
    • 1
  • W. Herzog
    • 2
    • 3
  • S. K. Han
    • 2
  • N. A. Abu Osman
    • 1
  • B. Pingguan-Murphy
    • 1
  • S. Federico
    • 3
  1. 1.Department of Biomedical Engineering, Faculty of EngineeringUniversity of MalayaKuala LumpurMalaysia
  2. 2.Human Performance Laboratory, Faculty of KinesiologyThe University of CalgaryCalgaryCanada
  3. 3.Department of Mechanical and Manufacturing Engineering, Schulich School of EngineeringThe University of CalgaryCalgaryCanada

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