Rapid Growth of Cartilage Rudiments may Generate Perichondrial Structures by Mechanical Induction
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Experimental and theoretical research suggest that mechanical stimuli may play a role in morphogenesis. We investigated whether theoretically predicted patterns of stress and strain generated during the growth of a skeletal condensation are similar to in vivo expression patterns of chondrogenic and osteogenic genes. The analysis showed that predicted patterns of compressive hydrostatic stress (pressure) correspond to the expression patterns of chondrogenic genes, and predicted patterns of tensile strain correspond to the expression patterns of osteogenic genes. Furthermore, the results of iterative application of the analysis suggest that stresses and strains generated by the growing condensation could promote the formation and refinement of stiff tissue surrounding the condensation, a prediction that is in agreement with an observed increase in collagen bundling surrounding the cartilage condensation, as indicated by picro-sirius red staining. These results are consistent with mechanical stimuli playing an inductive or maintenance role in the developing cartilage and associated perichondrium and bone collar. This theoretical analysis provides insight into the potential importance of mechanical stimuli during the growth of skeletogenic condensations.
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- Albrecht U, Helms JA, Lin H, Eichele G (1997) In: Daston GP (eds) Molecular and Celluar methods in developmental toxicology. CRC Press, Boca Raton, pp 23–48Google Scholar
- Claes LE, Heigele CA, Neidlinger-Wilke C, Kaspar D, Seidl W, Margevicius KJ, Augat P (1998) Effects of mechanical factors on the fracture healing process. Clin Orthop (355 Suppl):S132–S147Google Scholar
- Folkman J, Greenspan HP (1975) Influence of geometry on control of cell growth. Biochim Biophys Acta 417(3–4):211–236Google Scholar
- Oster GF, Murray JD, Harris AK (1983) Mechanical aspects of mesenchymal morphogenesis. J Embryol Exp Morphol 78:83–125Google Scholar
- Otto F, Thornell AP, Crompton T, Denzel A, Gilmour KC, Rosewell IR, Stamp GW, Beddington RS, Mundlos S, Olsen BR, Selby PB, Owen MJ (1997) Cbfa1, a candidate gene for cleidocranial dysplasia syndrome, is essential for osteoblast differentiation and bone development. Cell 89(5):765–771CrossRefGoogle Scholar
- Pauwels F (1980) Biomechanics of the locomotor apparatus. Springer, Berlin Heidelberg New YorkGoogle Scholar
- Perren SM, Cordey J (1980) The concept of interfragmentary strain. In: Uhthoff HK (eds) Current concepts of internal fixation of fractures. Springer, Berlin Heidelberg New York, vol. 23. pp 63–77Google Scholar
- Takahashi I, Nuckolls GH, Takahashi K, Tanaka O, Semba I, Dashner R, Shum L, Slavkin HC (1998) Compressive force promotes sox9, type II collagen and aggrecan and inhibits IL-1beta expression resulting in chondrogenesis in mouse embryonic limb bud mesenchymal cells. J Cell Sci 111 (Pt 14):2067–2076Google Scholar
- Wren TA, Beaupre GS, Carter DR (2000) Mechanobiology of tendon adaptation to compressive loading through fibrocartilaginous metaplasia. J Rehabil Res Dev 37(2):135–143Google Scholar
- Yamada H (1970) Strength of biological materials. Gaynor Evans F (ed) Waverly, BaltimoreGoogle Scholar