Ichthyological Research

, Volume 66, Issue 1, pp 183–188 | Cite as

Origin of the widely and discontinuously distributed mitochondrial genotypes of Oryzias latipes: introduced or native genotypes?

  • Yuka Iguchi
  • Ryohei Nakao
  • Masaru Matsuda
  • Keisuke Takata
  • Tadao KitagawaEmail author
Short Report


A mitochondrial cytochrome b genotype of Oryzias latipes, “mitotype B15”, is widely and discontinuously distributed in various localities in Japan. To re-examine the origin of mitotype B15 detected in various localities, we conducted a sequence analysis of the NADH dehydrogenase subunit 2 (ND2) gene region. Twenty-eight haplotypes were defined among mitotype B15 fish from 23 localities throughout Japan. Most of the haplotypes detected in each area are unique to the location and genetically closely related to each other, indicating that they are native haplotypes. Our results suggest that ND2 analysis is important for identifying native populations of O. latipes.


Conservation Primary freshwater fish ND2 



We thank Drs. Makito Kobayashi (International Christian University), Ryuya Okada (Kindai University), Mrs. Tomiji Hagiwara (Global Environmental Forum), Masato Fukui (Environmental Management Association of Ibaraki Prefecture), and Hikotarou Ida (Chiba Prefecture) for their help with sample collections. We are also thankful for the technical assistance from Ms. Yu Kawai, Ms. Nana Motokage, and Ms. Hazuki Nakamae (Kindai University). We express our gratitude to the two anonymous referees for providing valuable comments. This study complies with the current laws of Japan.

Supplementary material

10228_2018_662_MOESM1_ESM.pptx (154 kb)
Supplementary material 1 (PPTX 153 kb)
10228_2018_662_MOESM2_ESM.docx (44 kb)
Supplementary material 2 (DOCX 45 kb)


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Copyright information

© The Ichthyological Society of Japan 2018

Authors and Affiliations

  1. 1.Program in Environmental Management, Graduate School of AgricultureKindai UniversityNaraJapan
  2. 2.Graduate School of Human Development and EnvironmentKobe UniversityKobeJapan
  3. 3.Center for Bioscience Research and EducationUtsunomiya UniversityUtsunomiyaJapan
  4. 4.Faculty of ScienceShinshu UniversityMatsumotoJapan

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