Ichthyological Research

, Volume 64, Issue 4, pp 478–480 | Cite as

Genetic and stable isotope analyses of threespine stickleback from the Bering and Chukchi seas

  • Manabu Kume
  • Jun Kitano
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Threespine sticklebacks, Gasterosteus aculeatus, are widely distributed in the Northern Hemisphere (Wootton 1984). Life histories of threespine stickleback include both anadromy and freshwater-residency (Bell and Foster 1994). Anadromous juveniles migrate to the sea in late summer or autumn to grow and adults migrate into rivers and lakes in spring to reproduce, although there is variation in the timing of spawning and seaward migration (see Tables 1 and 2 in Kitano et al. 2012). Otolith Sr/Ca analyses suggest intra-species variation in marine-freshwater habitat use (Arai et al. 2003). In addition, catch records of threespine stickleback in offshore waters of the Pacific and Atlantic Oceans show that they are found in a variety of water depths (<0.18 m–232 m) and distances from land (<5 km–945 km) and multiple oceanographic realms (shelf, slope and deep ocean) (see Table 3 in Kitano et al. 2012). However, knowledge of the ecology of anadromous threespine sticklebacks at the marine...


Stable Isotope Analysis Electronic Supplementary Material Table Threespine Stickleback Restriction Fragment Length Polymorphism Analysis Electronic Supplementary Material Appendix 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.



We thank Dr. K. Morita (Hokkaido National Fisheries Research Institute) for generously providing samples, Drs. T. Kokita (Fukui Prefectural University), A. Ishikawa, K. Yoshida and M. Ravinet (National Institute of Genetics) for their helpful comments during this study, and two anonymous reviewers for constructive comments on the earlier version of the manuscript. We also thank the Institute of Environmental Ecology, IDEA Consultants, Inc. for stable isotope analysis. This work was supported by JSPS KAKENHI Grant Number JP15H02418 to JK.

Supplementary material

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  1. Arai T, Goto A, Miyazaki N (2003) Migratory history of the threespine stickleback Gasterosteus aculeatus. Ichthyol Res 50:9–14Google Scholar
  2. Bell MA, Foster SA (1994) The evolutionary biology of the threespine stickleback. Oxford University Press, OxfordGoogle Scholar
  3. Chouvelan T, Spitz J, Caurant F, Mèndez-Fernandez P, Chappuis A, Laugier F, Le Goff E, Bustamante P (2012) Revisiting the use of δ15N in meso-scale studies of marine food webs by considering spatio-temporal variations in stable isotopic signatures—the case of an open ecosystem: the Bay of Biscay (North-East Atlantic). Prog Oceanogr 101:92–105Google Scholar
  4. Deagle BE, Reimchen TE. Levin DB (1996) Origins of endemic stickleback from the Queen Charlotte Islands: mitochondrial and morphological evidence. Can J Zool 74:1045–1056Google Scholar
  5. France RL (1995) Carbon-13 enrichment in benthic compared to planktonic algae: foodweb implications. Mar Ecol Prog Ser 124:307–312Google Scholar
  6. Higuchi M, Sakai H, Goto A (2014) A new threespine stickleback, Gasterosteus nipponicus sp. nov. (Teleostei: Gasterosteidae), from the Japan Sea region. doi 10.1007/s10228-014-0403-1 (also appeared in Ichthyol Res 61:341–351)Google Scholar
  7. Jones DH, John AWG (1978) A three-spined stickleback, Gasterosteus aculeatus L. from the North Atlantic. J Fish Biol 13:231–236Google Scholar
  8. Kitano J, Mori S, Peichel CL (2007) Sexual dimorphism in the external morphology of the threespine stickleback (Gasterosteus aculeatus). Copeia 2007:336–349Google Scholar
  9. Kitano J, Ishikawa A, Kume M, Mori S (2012) Physiological and genetic basis for variation in migratory behavior in the three-spined stickleback, Gasterosteus aculeatus. Ichthyol Res 59:293–303Google Scholar
  10. Lescak EA, Marcotte RW, Kenney LA, von Hippel FA, Cresko WA, Sherbick ML, Colgren JJ, López JA (2014) Admixture of ancient mitochondrial lineages in three-spined stickleback populations from the North Pacific. J Biogeogr 42:532–539Google Scholar
  11. Michener R, Lajtha K (2007) Stable isotopes in ecology and environmental science (2nd ed). Blackwell Publishing, OxfordGoogle Scholar
  12. Morita K, Sato S, Kato M, Yamamoto J (2009) The summer 2009 Japanese salmon research cruise of the R/V Hokko maru: exploration of the northern limit of offshore distribution and annual survey in the Bering Sea. NPAFC Doc 1191Google Scholar
  13. Ortí G, Bell MA, Reimchen TE, Meyer A (1994) Global survey of mitochondrial DNA sequences in the threespine stickleback: evidence for recent migrations. Evolution 48:608–622Google Scholar
  14. Peichel CL, Nereng KS, Ohgi KA, Cole BLE, Colosimo PF, Buerkle CA, Schluter D, and Kingsley DM (2001) The genetic architecture of divergence between threespine stickleback species. Nature 414:901–905Google Scholar
  15. Quinn TP, Light JT (1989) Occurrence of threespine stickleback (Gasterosteus aculeatus) in the open North Pacific Ocean: migration or drift? Can J Zool 67:2850–2852Google Scholar
  16. Schell MD, Barnett BA, Vinette KA (1998) Carbon and nitrogen isotope ratios in zooplankton of the Bering, Chukchi and Beaufort seas. Mar Ecol Prog Ser 162:11–23Google Scholar
  17. Vander Zanden MJ, Rasmussen JB (2001) Variation in δ13C and δ15N trophic fractionation: implications for aquatic food web studies. Limnol Oceanogr 46:2061–2066Google Scholar
  18. Williams DD, Delbeek C (1989) Biology of the threespine stickleback, Gasterosteus aculeatus, and the blackspotted stickleback, Gasterosteus wheatlandi, during their marine pelagic phase in the Bay of Fundy, Canada. Environ Biol Fishes 24:33–41Google Scholar
  19. Wootton RJ (1984) A functional biology of sticklebacks. Croom Helm, LondonGoogle Scholar

Copyright information

© The Ichthyological Society of Japan 2017

Authors and Affiliations

  1. 1.Division of Ecological GeneticsNational Institute of GeneticsMishimaJapan
  2. 2.Field Science Education and Research CenterKyoto UniversityKyotoJapan

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