Ichthyological Research

, Volume 62, Issue 3, pp 357–362 | Cite as

Studying the contribution of two types of landlocked Ayu fish in Lake Biwa to the next generation using nitrogen-stable isotope ratio analysis

  • Takuya ItoEmail author
  • Kentaro Matsumura
  • Genki Kozawa
  • Maho Ozawa
  • Yoshito Mitsuo
  • Atsushi Maruyama
  • Masahide Yuma
Short Report


Contribution of amphidromous (Ooayu) and lake types (Koayu) of the landlocked Ayu (Plecoglossus altivelis altivelis) fish population in Lake Biwa to the next generation was investigated by isotopic approaches. δ15N values of eggs in adult ovaries distinctively differed between the two types. Contribution of Koayu, calculated using δ15N values of spawned Ayu eggs, was much greater than that of Ooayu in general, as previously reported. Ooayu spawned more eggs in the upper area of the spawning ground and did so late in the season. These results show the potential of isotopic approaches for identifying maternal life-history types of spawned eggs of migratory animals.


Ayu egg Life history Plecoglossus altivelis altivelis Ovary Polymorphism 



The authors thank the Shiga Prefecture, the Kitafunaki and Hirose Fisheries Corporations of Ado River, Mitani and Hamabun Fisheries Corporations of Ishida River, the Minamihama Fisheries Corporation of Ane River, and the Amanogawa-cho Fisheries Corporation of Amano River for permission to conduct surveys. We thank Dr. Y. Onoda of the Aqua Restoration Research Center and Dr. H. Yamanaka of Ryukoku University for their fruitful discussions. The authors declare that all experiments comply with the current laws of Japan, where they were conducted.


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Copyright information

© The Ichthyological Society of Japan 2014

Authors and Affiliations

  • Takuya Ito
    • 1
    Email author
  • Kentaro Matsumura
    • 1
  • Genki Kozawa
    • 1
  • Maho Ozawa
    • 1
  • Yoshito Mitsuo
    • 1
  • Atsushi Maruyama
    • 1
  • Masahide Yuma
    • 1
  1. 1.Faculty of Science and TechnologyRyukoku UniversityOtsuJapan

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