Ichthyological Research

, Volume 59, Issue 1, pp 49–52 | Cite as

Change in body size of juvenile marbled sole Pseudopleuronectes yokohamae after preservation in ethanol

  • Jeong-Hoon LeeEmail author
  • Keita Kodama
  • Toshihiro Horiguchi
Full Paper


We evaluated the extent of shrinkage in body size of juvenile marbled sole Pseudopleuronectes yokohamae after preservation in 70% ethanol for 6, 12, 24, or 48 h, or 4 weeks. Standard length (SL, range of the analyzed specimens: 22.82–53.01 mm) decreased by 5.6% after 12 h and body weight (BW 0.174–2.964 g) decreased 27.8% after 24 h preservation in 70% ethanol. There was no further decrease in SL or BW after 12 or 24 h of preservation, respectively. The original body size could be estimated based on the size after preservation in 70% ethanol for 4 weeks using the following equations: SL original = 1.05 SL preserved + 0.37 and BW original = 1.36 BW preserved + 0.04. The condition factor calculated using the body size of preserved individuals was 16.0 and 15.8% lower than that calculated from the original body size and the body size back-calculated using the linear regression equations described above, respectively. Thus, our data suggest that the shrinkage of body size due to preservation in ethanol can cause errors in estimation of the condition factor. Our results can be used to improve the accuracy of estimating size-related biological parameters based on juvenile marbled sole that are preserved in ethanol.


Body size Ethanol Juvenile Preservation Pseudopleuronectes yokohamae 



We thank the staff of the Chiba Prefectural Fisheries Research Center as well as the fishermen of Chiba Prefecture for their help with field sampling.


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Copyright information

© The Ichthyological Society of Japan 2011

Authors and Affiliations

  • Jeong-Hoon Lee
    • 1
    Email author
  • Keita Kodama
    • 1
  • Toshihiro Horiguchi
    • 1
  1. 1.Center for Environmental Risk ResearchNational Institute for Environmental StudiesTsukubaJapan

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