Population structure and genetic diversity were examined using partial mitochondrial cytochrome b gene sequences of four wild, one reintroduced, and five captive populations of the endangered cyprinid Hemigrammocypris rasborella from three river systems in the easternmost region of the species’ range in Shizuoka Prefecture, central Honshu, Japan. We detected loss of genetic diversity from portions of the wild and captive populations, as well as suspected nonindigenous haplotypes in some captive, reintroduced, and even wild populations. Given the population structure revealed, we suggest that the populations should be managed with consideration for both the endemism and viability (avoidance of inbreeding depression) of the local populations.
Cyprinidae Captive population Mitochondrial cytochrome b (cyt b) Nested clade phylogeographic analysis
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We express our sincere thanks to N. Suzuki, H. Mizuno, S. Kaji, H. Narushima, K. Nakano, and M. Kobayashi for providing the specimens, and to M. Nishida for lending us experimental instruments. This study was partially supported by Grants-in-Aid from the Ministry of Education, Culture, Sports, Science, and Technology, Japan (nos. 18570086 and 21370035).
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