Molecular identification of dolphinfish species (genus Coryphaena) using multiplex haplotype-specific PCR of mitochondrial DNA
- 171 Downloads
A rapid and reliable method of dolphinfish species identification was designed based on PCR amplification of diagnostic DNA fragments from the mitochondrial cytochrome b gene. It consisted in a tetraplex reaction producing a positive control amplicon and species-specific fragments in Coryphaena hippurus and C. equiselis. It was successfully tested in specimens of known identity and in nominal C. hippurus samples among which two C. equiselis were discovered. This approach has significant advantages over other molecular species identification methods and may help in determining species composition of mixed catches, and in forensic and food control applications of dolphinfish specimens or products.
KeywordsMolecular species identification Cytochrome b MHS–PCR Coryphaena hippurus Coryphaena equiselis
We thank R. Cardeña (U. del Mar, Mexico), J. Hyde and R.D. Vetter (NOAA-SWFSC, USA) for kindly contributing tissue samples. Financial support came from grant SAGARPA-CONACYT 2003-C01-42. J.P. Chávez González was financed by the “Programa Delfin,” which is gratefully acknowledged. We thank M. Bobadilla, J. Sandoval, and N. Saavedra for technical support. All experiments reported comply with the current laws of Mexico.
- Campos JA, Segura A, Lizano O, Madrigal E (1993) Basic ecology of Coryphaena hippurus (Pisces: Coryphaenidae) and abundance of other large pelagic organisms in the Pacific of Costa Rica. Rev Biol Trop 41:783–790Google Scholar
- Cronin MA, Palmisciano DA, Vyse ER, Cameron DG (1991) Mitochondrial DNA in wildlife forensic science species identification of tissues. Wildl Soc Bull 19:94–105Google Scholar
- Herzig CE (1990) Mitochondrial DNA used to track the dispersal of a preferred menu item in Waikiki restaurants: Mahimahi as an example of genetic tagging with mtDNA. Pac Sci 44:186–187Google Scholar
- Vaca-Rodriguez JG, Enriquez-Andrade RR (2002) Multicriteria analysis of log-set strata closures to reduce the Mexican tuna fishery incidental catch. Cienc Mar 28:257–271Google Scholar