Ichthyological Research

, Volume 54, Issue 3, pp 253–261 | Cite as

Comparative phylogeography of bagrid catfishes in Taiwan

  • Katsutoshi Watanabe
  • Nian-Hong Jang-Liaw
  • Chun-Guang Zhang
  • Sang-Rin Jeon
  • Mutsumi Nishida


A phylogeographic analysis of two bagrid catfishes in Taiwan was conducted using sequence data from a portion of the mitochondrial DNA (mtDNA) control region. For Pseudobagrus brevianalis, which is most probably endemic to Taiwan, a total of eight haplotypes were detected in 189 specimens from nine river systems covering its entire distribution range, from northern to central western areas of the island. Obvious genetic differentiation was observed among its populations (average F ST = 0.753); in particular, the northernmost Tamsui River population was fixed for a single endemic haplotype. Nested clade phylogeographic analysis (NCPA) indicated that the dispersal center of mtDNA was the area around the Touchien River and Holong River, north to the Miaoli Plateau, in northwestern Taiwan, suggesting both northward and southward dispersal in this species. There was no evidence for the validity of P. taiwanensis, the nominal species described from Taiwan, morphologically similar to P. brevianalis. We confirmed that P. adiposalis was distributed discontinuously in three river systems; analysis of 42 specimens from the rivers indicated a total of four haplotypes and population differentiation (average F ST = 0.876). Fixation into a largely differentiated haplotype in the northernmost Tamsui River population was also found in this species, but different processes for this phylogeographic pattern were implied for the two species. Comparison with P. ussuriensis, a widespread continental species morphologically similar to P. adiposalis, suggested the possibility that P. adiposalis is a group of local populations of P. ussuriensis in Taiwan. Two migration routes vs. random fixation scenarios for the population structure of P. adiposalis are discussed with information on other fishes and the geological history of the island.

Key words

Control region mtDNA Pseudobagrus adiposalis Pseudobagrus brevianalis Pseudobagrus ussuriensis 


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Avise, JC 2000Phylogeography: the history and formation of speciesHarvard University PressCambridgeGoogle Scholar
  2. Chen IS, Fang LS (1999) The freshwater and estuarine fishes of Taiwan. National Museum of Marine Biology & Aquarium, PingtungGoogle Scholar
  3. Chu, YT 1984Fishes of Fujian Province. Part 1Fujian Science & Technology PressFujian ProvinceGoogle Scholar
  4. Clement, M, Posada, D, Crandall, K 2000TCS: a computer program to estimate gene genealogiesMol Ecol916571660PubMedCrossRefGoogle Scholar
  5. Creer, S, Malhotra, A, Thorpe, RS, Chou, WH 2001Multiple causation of phylogeographical pattern as revealed by nested clade analysis of the bamboo viper (Trimeresurus stejnegeri) within TaiwanMol Ecol1019671981PubMedCrossRefGoogle Scholar
  6. Dybowski, BI 1872Zur Kenntniss der Fischfauna des AmurgebietesVerh K-K Zool-Bot Ges Wien22209222Google Scholar
  7. Excoffier, L, Laval, G, Schneider, S 2005Arlequin ver. 3.0: an integrated software package for population genetics data analysisEvol Bioinform Online14750Google Scholar
  8. Gao, GF 1991Siluriformes: BagridaePan, JH eds. The freshwater fishes of Guangdong ProvinceGuangdong Science & Technology PressGuangdong297317Google Scholar
  9. Iguchi, K, Tanimura, Y, Nishida, M 1997Sequence divergence in the mtDNA control region of amphidromous and landlocked forms of ayuFish Sci63901905Google Scholar
  10. Inoue, JG, Miya, M, Tsukamoto, K, Nishida, M 2000Complete mitochondrial DNA sequence of the Japanese sardine, Sardinops melanostictus Fish Sci66924932CrossRefGoogle Scholar
  11. Lee, PF 2004Natural resource and ecological database of Taiwan (1): biodiversityCouncil of AgricultureTaipeiGoogle Scholar
  12. Leviton, AE, Gibbs, RH,Jr, Heal, E, Dawson, CE 1985Standards in herpetology and ichthyology: part I. Standard symbolic codes for institutional resource collections in herpetology and ichthyologyCopeia1985802832Google Scholar
  13. Li, J, Chen, X, Chan, BPL 2005A new species of Pseudobagrus (Teleostei: Siluriformes: Bagridae) from southern ChinaZootaxa10674957Google Scholar
  14. Mo, T 1991Anatomy and systematics of Bagridae (Teleostei), and siluroid phylogenyKoeltzKoenigsteinGoogle Scholar
  15. Oshima, M 1919Contributions to the study of the fresh water fishes of the island of FormosaAnn Carnegie Mus12169328pls 48–53Google Scholar
  16. Oshima, M 1923Distribution of the freshwater fishes of Taiwan and geographical relationships between the Taiwan Island and adjacent areasZool Mag (Tokyo)35149Google Scholar
  17. Posada, D, Crandall, KA 1998Modeltest: testing the model of DNA substitutionBioinformatics14817818PubMedCrossRefGoogle Scholar
  18. Posada, D, Crandall, KA, Templeton, AR 2000GeoDis: a program for the cladistic nested analysis of the geographical distribution of genetic haplotypesMol Ecol9487488PubMedCrossRefGoogle Scholar
  19. Regan, CT 1908Descriptions of new freshwater fishes from China and JapanAnn Mag Nat Hist Ser8149153Google Scholar
  20. Shao KT, Yen HW, Yu YL, Lin CJ (2006) Taiwan biodiversity national information network. Version 2006/1. Scholar
  21. Shen, CF 1996Biogeography of Taiwan: 1. BackgroundAnn Taiwan Mus39287427Google Scholar
  22. Sivasundar, A, Bermingham, E, Ortí, G 2001Population structure and biogeography of migratory freshwater fishes (Prochilodus: Characiformes) in major South American riversMol Ecol10407417PubMedCrossRefGoogle Scholar
  23. Swofford DL (2002). PAUP*. Phylogenetic analysis using parsimony (*and other methods), version 4b10a. Sinauer Associates, Sunderland, MAGoogle Scholar
  24. Templeton, AR 1998Nested clade analyses of phylogeographic data: testing hypotheses about gene flow and population historyMol Ecol7381397PubMedCrossRefGoogle Scholar
  25. Templeton, AR, Sing, CF 1993A cladistic analysis of phenotypic associations with haplotypes inferred from restriction endonuclease mapping and DNA sequence data. IV. Nested analyses with cladogram uncertainty and recombinationGenetics134659669PubMedGoogle Scholar
  26. Templeton, AR, Crandall, KA, Sing, CF 1992A cladistic analysis of phenotypic associations with haplotypes inferred from restriction endonuclease mapping and DNA sequence data. III. Cladogram estimationGenetics132619633PubMedGoogle Scholar
  27. Tzeng, CS 1986Distribution of the freshwater fishes of TaiwanJ Taiwan Mus39127146Google Scholar
  28. Wang, HY, Tsai, MP, Yu, MJ, Lee, SC 1999Influence of glaciation on divergence patterns of the endemic minnow, Zacco pachycephalus in TaiwanMol Ecol818791888PubMedCrossRefGoogle Scholar
  29. Wang, JP, Hsu, KC, Chiang, TY 2000Mitochondrial DNA phylogeography of Acrossocheilus paradoxus (Cyprinidae) in TaiwanMol Ecol914831494PubMedCrossRefGoogle Scholar
  30. Wang, JP, Lin, HD, Huang, S, Pan, CH, Chen, XL, Chiang, TY 2004Phylogeography of Varicorhinus barbatulus (Cyprinidae) in Taiwan based on nucleotide variation of mtDNA and allozymesMol Phylogenet Evol3111431156PubMedCrossRefGoogle Scholar
  31. Watanabe K (1995) Natural history of the Japanese bagrid catfish, Pseudobagrus ichikawai. PhD thesis, Tokyo University of Fisheries, TokyoGoogle Scholar
  32. Watanabe, K, Maeda, H 1995Redescription of two ambiguous Japanese bagrids, Pseudobagrus aurantiacus (Temminck and Schlegel) and P. tokiensis DöderleinJpn J Ichthyol41409420Google Scholar
  33. Watanabe, K, Nishida, M 2003Genetic population structure of Japanese bagrid catfishesIchthyol Res50140148CrossRefGoogle Scholar
  34. Yang, YJ, Lin, YS, Wu, JL, Hui, CF 1994Variation in mitochondrial DNA and population structure of the Taipei tree frog Rhacophorus taipeianus in TaiwanMol Ecol3219228PubMedGoogle Scholar
  35. Yu, HT, Peng, YH 2002Population differentiation and gene flow revealed by microsatellite DNA markers in the house mouse (Mus musculus castaneus) in TaiwanZool Sci19475483PubMedCrossRefGoogle Scholar
  36. Zhang, CL 1960Fauna of Chinese catfish (Siluriformes)People's Education PressBeijingGoogle Scholar
  37. Zheng, BS, Dai, DY 1999BagridaeChu, XLZheng, BSDai, DY eds. Fauna Sinica: Osteichthyes: SiluriformesScience PressBeijing3574Google Scholar
  38. Zheng, CY 1989Fishes of the Zhujiang RiverScience PressBeijingGoogle Scholar

Copyright information

© The Ichthyological Society of Japan 2007

Authors and Affiliations

  • Katsutoshi Watanabe
    • 1
  • Nian-Hong Jang-Liaw
    • 2
  • Chun-Guang Zhang
    • 3
  • Sang-Rin Jeon
    • 4
  • Mutsumi Nishida
    • 5
  1. 1.Department of Zoology, Division of Biological ScienceGraduate School of Science, Kyoto UniversityKyotoJapan
  2. 2.Department of ZoologyNational Museum of Natural ScienceTaichungTaiwan
  3. 3.Institute of ZoologyChinese Academy of SciencesBeijingP.R. China
  4. 4.Department of BiologyCollege of Natural Sciences, Sang Myung UniversitySeoulKorea
  5. 5.Ocean Research InstituteThe University of TokyoTokyoJapan

Personalised recommendations