Ichthyological Research

, Volume 53, Issue 4, pp 427–430 | Cite as

Gonadal morphology in the self-fertilizing mangrove killifish, Kryptolebias marmoratus

  • Yoshitaka SakakuraEmail author
  • Kiyoshi Soyano
  • David L.G. Noakes
  • Atsushi Hagiwara
Short Report


We conducted anatomical and histological observations of the gonads in the self-fertilizing mangrove killifish, Kryptolebias marmoratus to investigate the self-fertilizing mechanism of this species. The gonad has a bilobed structure. The elongated gonadal lumen (GL) along the dorsal surface of the gonad connects to the common genital sinus. The elongate testicular region is closely attached to the GL. Among the ovulated eggs in the GL, those in the anterior part of the GL have micropyles but no perivitelline space (are not yet fertilized), whereas those in the posterior part of the GL are fertilized. In our histological analysis, we found free sperm in the posterior area of the GL. We conclude that ovulated eggs may be self-fertilized in the posterior GL.

Key words

Self-fertilization Histology Hermaphrodite Rivulus marmoratus Gonad 


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Copyright information

© The Ichthyological Society of Japan 2006

Authors and Affiliations

  • Yoshitaka Sakakura
    • 1
    Email author
  • Kiyoshi Soyano
    • 2
  • David L.G. Noakes
    • 3
  • Atsushi Hagiwara
    • 4
  1. 1.Faculty of FisheriesNagasaki UniversityNagasakiJapan
  2. 2.Institute for East China Sea ResearchNagasaki UniversityNagasakiJapan
  3. 3.Institute of Ichthyology, Department of ZoologyUniversity of GuelphOntarioCanada
  4. 4.Graduate School of Science and TechnologyNagasaki UniversityNagasakiJapan

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