Ichthyological Research

, Volume 53, Issue 1, pp 87–92 | Cite as

Parentage analyses of freshwater goby Rhinogobius sp. OR using microsatellite DNA markers and egg developmental stages of eggs in the nest

  • Daisuke Takahashi
  • Kenichi Ohara
Short report


Parentage analyses of the paternally caring goby Rhinogobius sp. OR were performed using microsatellite DNA markers and examination of developmental stages of eggs collected from five nests with parental males in the wild. Four of five nests had egg masses with eggs at the same developmental stages from single females. In one nest, the egg mass with eggs at three different developmental stages originated from four females, and eggs of whole developmental stages were observed within the egg mass of each female. This observation suggests that in this goby embryonic stage is an inaccurate indicator of the number of mates.

Key words

Egg developmental stage Microsatellite DNA Nest spawner Parentage Rhinogobius 


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Copyright information

© The Ichthyological Society of Japan 2006

Authors and Affiliations

  1. 1.Laboratory of Animal Ecology, Department of Zoology, Graduate School of ScienceKyoto UniversityKyotoJapan
  2. 2.Lake Biwa MuseumShigaJapan

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