Ichthyological Research

, Volume 53, Issue 1, pp 75–81 | Cite as

New primers for amplifying and sequencing the mitochondrial ND4/ND5 gene region of the Cypriniformes (Actinopterygii: Ostariophysi)

  • Masaki Miya
  • Kenji Saitoh
  • Robert Wood
  • Mutsumi Nishida
  • Richard L. Mayden
Short report


We provide 15 new primers for amplifying and sequencing the mitochondrial ND4/ND5 gene region of the Cypriniformes in an attempt to resolve relationships of this diverse group of freshwater fishes with extensive taxonomic sampling. Sequences from this region have the following desirable characteristics for phylogenetic analyses, some of which are lacking from the more commonly used cyt b and 12S/16S rRNA genes: they are (1) easy to align, (2) relatively long (ca. 3.4 kb), and (3) contain more phylogenetically informative variation at 1st and 2nd codon positions. Moreover, the ND4/ND5 gene region is easy to amplify and sequence when employing the protocol suggested herein.

Key words

Cypriniformes Tree of Life (CToL) Molecular systematics Phylogeny 


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Copyright information

© The Ichthyological Society of Japan 2006

Authors and Affiliations

  • Masaki Miya
    • 1
  • Kenji Saitoh
    • 2
  • Robert Wood
    • 3
  • Mutsumi Nishida
    • 4
  • Richard L. Mayden
    • 3
  1. 1.Department of ZoologyNatural History Museum & InstituteChibaJapan
  2. 2.Tohoku National Fisheries Research InstituteMiyagiJapan
  3. 3.Department of BiologySt. Louis UniversitySt. LouisUSA
  4. 4.Ocean Research InstituteUniversity of TokyoTokyoJapan

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