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Application of environmental DNA metabarcoding in a lake with extensive algal blooms

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Abstract

Recently, environmental DNA (eDNA) metabarcoding techniques have been applied to biodiversity investigations in aquatic ecosystems. However, no study has yet tested whether this technique is effective for water bodies in which extensive algal blooms break out. In this study, fish eDNA metabarcoding was carried out in Lake Taihu, which experiences extensive algal blooms, to confirm whether the technique is also effective for fish diversity research in ecosystems with frequent and extensive blooms. In December 2016, three samples were collected, including one collected in the presence of algal blooms and two collected in the absence of algal blooms. In August 2017, six samples were collected, including three collected in the presence of algal blooms and three in the absence of algal blooms. Equal amount of water samples (1 L) was collected from each site; however, the actual amount of filtrate varied with the site. Twenty-seven freshwater fish species were detected from the water samples collected in Lake Taihu. The results showed that the composition of the detected species did not differ whether or not blooms were present. However, the amount of filtration could influence the number of species detected. The results suggest that future eDNA metabarcoding studies under similar water environments should increase the amount of filtration to maximize number of species detected.

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Acknowledgements

We are grateful to the students of Kobe University and Shanghai Jiao Tong University, who helped with water sampling. This work was partly supported by the Japan Society for Promotion of Science (JSPS) KAKENHI (Grant numbers: JP17H03735 and JP20H03326) and by the Environmental Research and Technology Development Fund (JPMEERF20S20704) of the Ministry of the Environment, Japan.

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  1. Qianqian Wu and Masayuki K. Sakata equally contributed to the study.

Authors and Affiliations

  1. Graduate School of Human Development and Environment, Kobe University, 3-11 Tsurukabuto, Nada-ku, Kobe, Hyogo, 657-8501, Japan

    Qianqian Wu, Masayuki K. Sakata & Toshifumi Minamoto

  2. School of Environmental Science and Engineering, Shanghai Jiao Tong University, 800 Dongchuan Rd., Shanghai, 200240, China

    Deyi Wu

  3. Faculty of Advanced Science and Technology, Ryukoku University, 1-5 Yokotani, Seta-Oe Cho, Otsu, Shiga, 520-2194, Japan

    Hiroki Yamanaka

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  1. Qianqian Wu
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  2. Masayuki K. Sakata
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  3. Deyi Wu
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Correspondence to Qianqian Wu.

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Wu, Q., Sakata, M.K., Wu, D. et al. Application of environmental DNA metabarcoding in a lake with extensive algal blooms. Limnology 22, 363–370 (2021). https://doi.org/10.1007/s10201-021-00663-1

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