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Limnology

, Volume 20, Issue 1, pp 39–53 | Cite as

The existence of cyanobactericidal bacteria and growth-inhibiting bacteria on water plants in Lake Ohnuma, Japan

  • Yohei MiyashitaEmail author
  • Takumi Hagiwara
  • Ichiro Imai
Special Feature Ecological and limnological bases for management of overgrown macrophytes

Abstract

Microorganisms such as bacteria are considered to be control agents against toxic cyanobacterial blooms such as Microcystis aeruginosa. We investigated cyanobactericidal bacteria and growth-inhibiting bacteria against M. aeruginosa in the biofilm on the surface of the water plants Trapa japonica, Myriophyllum verticallatum and Utricularia vulgaris at three sites in Ohnuma Quasi-National Park during the period from July to October 2012. Bacteria were isolated using nutrient agar plates, and killing abilities and growth-inhibiting abilities of isolated bacteria were examined for M. aeruginosa with co-culture experiments. Effective bacteria (sums of cyanobactericidal bacteria and growth-inhibiting bacteria) were confirmed from the leaves and water roots of T. japonica with densities of 3.6 × 105 CFU g−1 wet weight—1.2 × 107 CFU g−1 wet weight and 1.5 × 106 CFU g−1 wet weight—1.4 × 108 CFU g−1 wet weight, respectively. M. verticillatum and U. vulgaris harbored effective bacteria with densities of 2.5 × 106 CFU g−1 wet weight—1.1 × 107 CFU g−1 wet weight and 2.3 × 106 CFU g−1 wet weight—9.2 × 106 CFU g−1 wet weight, respectively. Effective bacteria were also detected from water samples collected from all three sites and the most numerous values were detected at Sansui with abundant water plants such as T. japonica and M. verticillatum. Densities of M. aeruginosa tended to be fewer at Sansui and were more abundant at Ohnuma Park with almost no water plants. The results of PCA suggested that the absence and/or lower densities of M. aeruginosa was closely related to the abundant presence of effective bacteria detected from the water and biofilms of water plants such as T. japonica at Sansui. The present findings provide new insights on the ecology of cyanobactericidal bacteria and growth-inhibiting bacteria, and suggest that water plants provide an environment that influences the abundance of cyanobacterial blooms in freshwater ecosystems.

Keywords

Cyanobactericidal bacteria Growth-inhibiting bacteria Microcystis Cyanobacterial blooms Water plants Trapa japonica 

Notes

Acknowledgements

We would like to express our sincere gratitude to Professor Kuniaki Tanaka of the Environmental Science Course, Hokkaido University of Education, Hakodate Campus for helpful advice during this research. We express our sincere thanks to those at Ohnuma Boating Co., Ltd. for overall cooperation with this research. We are grateful to Mr. Tsukasa Miyazaki of Ohnuma Fisheries Cooperative Association for his helpful information on Lake Ohnuma. We appreciate Messrs. Keigo Sekiguchi, Taku Kosugi and Akira Kobayashi of Nanae Town Office for their diligent cooperation with this study.

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Copyright information

© The Japanese Society of Limnology 2018

Authors and Affiliations

  1. 1.Plankton Laboratory, Graduate School of Fisheries SciencesHokkaido UniversityHakodateJapan

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