Determination of aquatic humic substances in Japanese lakes and wetlands by the carbon concentration-based resin isolation technique

Limnology volume 17, pages1–6(2016)Cite this article

Abstract

Aquatic humic substances (AHSs) are major constituents of dissolved organic matter (DOM) in freshwater bodies. We performed quantitative analyses of AHSs in Japanese lake and wetland waters, focusing mainly on clear waters with low carbon contents, by using a resin isolation technique based on the carbon concentration in the AHSs of each sample. The proportion of AHS to DOM in the clear waters ranged from 38.4 to 64.1 %; these proportions are lower than those widely assumed for freshwater of 20–80 %. Moreover, the proportions of AHSs in DOM were not constant, so regression analysis cannot be used to predict the AHS concentration from the DOM concentration. Thus, AHS and DOM concentrations must be determined separately for each water sample.

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References

  1. Aiken GR (1985) Humic substances in soil, sediment, geochemistry, isolation characterization. In: Aiken GR, McKnight DM, Wershaw RL, MacCarthy P (eds). Wiley, New York, p 363

  2. Aoki S, Ohara S, Kimura K, Mizuguchi H, Fuse Y, Yamada E (2008) Characterization of dissolved organic matter related from Microcystis aeruginosa. Anal Sci 24:389–394

    Article  CAS  PubMed  Google Scholar 

  3. Baron J, McKnight D, Denning AS (1991) Sources of dissolved and particulate organic material in Loch Vale Watershed, Rocky Mountain National Park, Colorado, USA. Biogeochemistry 15:89–110

    Article  CAS  Google Scholar 

  4. Cawley KM, McKnight DM, Miller P, Cory R, Fimmen RL, Guerard J, Dieser M, Jaros C, Chin Y, Foreman C (2013) Characterization of fulvic acid fractions of dissolved organic matter during ice-out in a hyper-eutrophic, coastal pond in Antarctica. Environ Res Lett 8:045015 (pp 10)

    Article  Google Scholar 

  5. Chanudet V, Filella M (2007) Submicron organic matter in a peri-alpine, ultra-oligotrophic lake. Org Geochem 38:1146–1160

    Article  CAS  Google Scholar 

  6. Ertel JR, Hedges JI, Devol AH, Richey JE, Ribeiro MNG (1986) Dissolved humic substances of the Amazon River system. Limnol Oceanogr 31:739–754

    Article  CAS  Google Scholar 

  7. Filella M, Rodriguez-Murillo JC, Quentel F (2013) Natural organic matter quantification in the water of a semiarid freshwater wetland (Tablas de Daimiel, Spain). J Environ Sci 25:114–123

    Article  CAS  Google Scholar 

  8. Fillela M (2010) Quantifying ‘humics’ in freshwater: purpose and methods. Chem Ecol 26:177–186

    Article  Google Scholar 

  9. Hansell DA, Carlson CA (2001) Marine dissolved organic matter and the carbon cycle. Oceanography 14:41–49

    Article  Google Scholar 

  10. Imai A, Fukushima T, Matsushige K, Kim YH (2001) Fractionation and characterization of dissolved organic matter in a shallow eutrophic lake, its inflowing rivers, and other organic matter sources. Water Res 35:4019–4028

    Article  CAS  PubMed  Google Scholar 

  11. Ishiwatari R, Yonebayashi K, Miyazima T (2008) Humic substances in environment—their characteristics and investigative approach. Sankyo Publishing Co., Ltd, pp 30–32 (in Japanese)

  12. Koch BP, Witt M, Engbrodt R, Drrtmar T, Kattner G (2005) Molecular formulae of marine and terrigenous dissolved organic matter detected by electrospray ionization Fourier transform ion cyclotron resonance mass spectrometry. Geochim Cosmochim Acta 69:3299–3308

    Article  CAS  Google Scholar 

  13. Lu XQ, Maie N, Hanna JV, Childers DL, Jaffe R (2003) Molecular characterization of dissolved organic matter in freshwater wetlands of the Florida Everglades. Water Res 37:2599–2606

    Article  CAS  PubMed  Google Scholar 

  14. Malcolm RL, MacCarthy P (1992) Quantitative evaluation of XAD-8 and XAD-4 resins used in tandem for removing organic solutes from water. Environ Inter 18:597–607

    Article  CAS  Google Scholar 

  15. McKnight DM, Aiken GR, Smith RL (1991) Aquatic fulvic acids in microbially-based ecosystems: results from two Antarctic desert lake. Limnol Oceanogr 36:998–1006

    Article  CAS  Google Scholar 

  16. Peuravuori J, Pihlaja K (1997) Isolation and characterization of natural organic matter from lake water: comparison of isolation with solid adsorption and tangential membrane filtration. Environ Inter 23:441–451

    Article  CAS  Google Scholar 

  17. Peuravuori J, Lehtonen T, Kalevi P (2002) Sorption of aquatic humic matter by DAX-8 and XAD-8 resins: comparative study using pyrolysis gas chromatography. Anal Chim Acta 471:219–226

    Article  CAS  Google Scholar 

  18. Steinberg C, Muenster U (1985) Geochemistry and ecological role of humic substances in lakewater. In: Aiken GR, McKnight DM, Wershaw RL, MacCarthy P (eds) Humic substances in soil, sediment, and water: geochemistry, isolation and characterization. Wiley, New York, pp 105–145

    Google Scholar 

  19. Sugiyama Y, Anegawa A, Inokuchi H, Kumagai T (2005) Distribution of dissolved organic carbon and dissolved fulvic acid in mesotrophic Lake Biwa, Japan. Limnology 6:161–168

    Article  CAS  Google Scholar 

  20. Thacker SA, Tipping E, Baker A, Gondar D (2005) Development and application of functional assays for freshwater dissolved organic matter. Water Res 39:4559–4573

    Article  CAS  PubMed  Google Scholar 

  21. Thurman EM (1985) Aquatic humic substances (chap. 10). In: Organic Geochem Natur Waters. Kluwer Academic Publishers Group, Dordrecht, pp 273–362

  22. Tsuda K, Mori H, Asakawa D, Yanagi Y, Kodama H, Nagao S, Yonebayashi K, Fujitake N (2010) Characterization and grouping of aquatic fulvic acids isolated from clear-water rivers and lakes in Japan. Water Res 44:3837–3846

    Article  CAS  PubMed  Google Scholar 

  23. Tsuda K, Takata A, Shirai H, Kozaki K, Fujitake N (2012) A method for quantitative analysis of aquatic humic substances in clear water based on carbon concentration. Anal Sci 28:1017–1020

    Article  CAS  PubMed  Google Scholar 

  24. Weishaar JL, Aiken GR, Bergamaschi BA, Fram MS, Fujii R, Mopper K (2003) Evaluation of specific ultraviolet absorbance as an indicator of the chemical composition and reactivity of dissolved organic carbon. Environ Sci Technol 37:4702–4708

    Article  CAS  PubMed  Google Scholar 

  25. Wu FC, Evans RD, Dillon PJ, Cai YR (2007) Rapid quantification of humic and fulvic acids by HPLC in natural waters. Appl Geochem 22:1598–1605

    Article  CAS  Google Scholar 

  26. Zomeren A, Comans RNJ (2007) Measurement of humic and fulvic acid concentrations and dissolution properties by a rapid batch procedure. Environ Sci Technol 41:6755–6761

    Article  PubMed  Google Scholar 

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Acknowledgments

This work was supported by a Grant-in-Aid for Scientific Research (C-24510044, 2012–2014).

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Author notes

  1. Kumiko Tsuda

    Present address: Kusatsu City Office, 3-13-30 Kusatsu, Kusatsu, Shiga, 525-8588, Japan

Affiliations

  1. Graduate School of Science and Technology, Kobe University, 1 Rokkodai, Kobe, Hyogo, 657-8501, Japan

    Kumiko Tsuda

  2. Graduate School of Agricultural Science, Kobe University, 1 Rokkodai, Kobe, Hyogo, 657-8501, Japan

    Morimaru Kida, Suzuka Aso, Taku Kato & Nobuhide Fujitake

  3. Department of Environmental Science, The University of Shiga Prefecture, 2500 Hassaka-cho, Hikone, Shiga, 522-8533, Japan

    Masahiro Maruo

  4. Lake Biwa Environmental Research Institute, 5-34 Yanagasaki, Otsu, Shiga, 520-0022, Japan

    Kazuhide Hayakawa

  5. Graduate School of Life and Environmental Sciences, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, Ibaraki, 305-8577, Japan

    Mitsuru Hirota

Authors
  1. Kumiko Tsuda
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  2. Morimaru Kida
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  3. Suzuka Aso
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  5. Nobuhide Fujitake
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  6. Masahiro Maruo
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  7. Kazuhide Hayakawa
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  8. Mitsuru Hirota
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Correspondence to Nobuhide Fujitake.

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Handling Editor: Youhei Yamashita.

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Tsuda, K., Kida, M., Aso, S. et al. Determination of aquatic humic substances in Japanese lakes and wetlands by the carbon concentration-based resin isolation technique. Limnology 17, 1–6 (2016). https://doi.org/10.1007/s10201-015-0455-6

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Keywords

  • Dissolved organic matter (DOM)
  • Aquatic humic substance (AHS)
  • Clear water
  • Quantitative analysis
  • Resin isolation technique