, Volume 17, Issue 1, pp 1–6 | Cite as

Determination of aquatic humic substances in Japanese lakes and wetlands by the carbon concentration-based resin isolation technique

  • Kumiko Tsuda
  • Morimaru Kida
  • Suzuka Aso
  • Taku Kato
  • Nobuhide Fujitake
  • Masahiro Maruo
  • Kazuhide Hayakawa
  • Mitsuru Hirota
Rapid communication Note on important and novel findings


Aquatic humic substances (AHSs) are major constituents of dissolved organic matter (DOM) in freshwater bodies. We performed quantitative analyses of AHSs in Japanese lake and wetland waters, focusing mainly on clear waters with low carbon contents, by using a resin isolation technique based on the carbon concentration in the AHSs of each sample. The proportion of AHS to DOM in the clear waters ranged from 38.4 to 64.1 %; these proportions are lower than those widely assumed for freshwater of 20–80 %. Moreover, the proportions of AHSs in DOM were not constant, so regression analysis cannot be used to predict the AHS concentration from the DOM concentration. Thus, AHS and DOM concentrations must be determined separately for each water sample.


Dissolved organic matter (DOM) Aquatic humic substance (AHS) Clear water Quantitative analysis Resin isolation technique 



This work was supported by a Grant-in-Aid for Scientific Research (C-24510044, 2012–2014).


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Copyright information

© The Japanese Society of Limnology 2015

Authors and Affiliations

  • Kumiko Tsuda
    • 1
    • 2
  • Morimaru Kida
    • 3
  • Suzuka Aso
    • 3
  • Taku Kato
    • 3
  • Nobuhide Fujitake
    • 3
  • Masahiro Maruo
    • 4
  • Kazuhide Hayakawa
    • 5
  • Mitsuru Hirota
    • 6
  1. 1.Graduate School of Science and TechnologyKobe UniversityKobeJapan
  2. 2.Kusatsu City OfficeKusatsuJapan
  3. 3.Graduate School of Agricultural ScienceKobe UniversityKobeJapan
  4. 4.Department of Environmental ScienceThe University of Shiga PrefectureHikoneJapan
  5. 5.Lake Biwa Environmental Research InstituteOtsuJapan
  6. 6.Graduate School of Life and Environmental SciencesUniversity of TsukubaTsukubaJapan

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