Limnology

, Volume 16, Issue 1, pp 69–77 | Cite as

Monitoring fish pathogenic viruses in natural lakes in Yunnan, China

  • Toshifumi Minamoto
  • Xiaoying Pu
  • Jie Xie
  • Yi Dong
  • Deyi Wu
  • Hainan Kong
  • Xiaoxia Yang
  • Teruhiko Takahara
  • Mie N. Honjo
  • Hiroki Yamanaka
  • Zen’ichiro Kawabata
Asia/Oceania report

Abstract

Outbreaks of infectious diseases among freshwater fish damage the aquaculture industry. A technique for detecting pathogens in environmental water samples has recently been developed. In the present work, the monitoring of pathogenic viruses in Lake Erhai and Lake Dianchi (Yunnan, China) was performed. Yunnan possesses a rich diversity of cyprinid fish, and the aquaculture of freshwater fish is a highly prosperous industry in this region. Thus, Cyprinid herpesvirus 1, 2, and 3 as well as three rhabdoviruses, Infectious hematopoietic necrosis virus, Viral hemorrhagic septicemia virus, and the Spring viremia of carp virus, all of which have severe impacts on aquaculture, were targeted. Viruses in lake surface water were concentrated and then quantified/detected by real-time PCR/real-time reverse transcription PCR. Five of the six tested viruses were detected in lake water. Although there has been no reported viral outbreak in the survey lakes, our results indicate a potential risk for these viral diseases.

Keywords

Cyprinid herpesviruses Infectious hematopoietic necrosis virus (IHNV) Spring viremia of carp virus (SVCV) Viral hemorrhagic septicemia virus (VHSV) Lake Erhai 

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Copyright information

© The Japanese Society of Limnology 2014

Authors and Affiliations

  • Toshifumi Minamoto
    • 1
    • 2
  • Xiaoying Pu
    • 3
  • Jie Xie
    • 4
  • Yi Dong
    • 4
  • Deyi Wu
    • 4
  • Hainan Kong
    • 4
  • Xiaoxia Yang
    • 3
  • Teruhiko Takahara
    • 1
    • 5
  • Mie N. Honjo
    • 1
    • 6
  • Hiroki Yamanaka
    • 7
  • Zen’ichiro Kawabata
    • 1
  1. 1.Research Institute for Humanity and NatureKyotoJapan
  2. 2.Graduate School of Human Development and EnvironmentKobe UniversityKobeJapan
  3. 3.Department of Agriculture and Biological ScienceDali UniversityDaliChina
  4. 4.School of Environmental Science and EngineeringShanghai Jiaotong UniversityShanghaiChina
  5. 5.Graduate School of Integrated Arts and SciencesHiroshima UniversityHigashi-HiroshimaJapan
  6. 6.Center for Ecological ResearchKyoto UniversityOtsuJapan
  7. 7.Faculty of Science and TechnologyRyukoku UniversityOtsuJapan

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