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Modern Rheumatology

, Volume 23, Issue 4, pp 804–810 | Cite as

Fc receptor beta chain deficiency exacerbates murine arthritis in the anti-type II collagen antibody-induced experimental model

  • Mino Ohtsubo-Yoshioka
  • Satoshi Nunomura
  • Tatsuki R. Kataoka
  • Yoshimichi Okayama
  • Chisei RaEmail author
Original Article
  • 236 Downloads

Abstract

Objective

Fc receptor β chain (FcRβ) acts as a signaling component of FcγRIII in immune cells such as mast cells (MCs) or basophils. Recent studies reported that FcγRIII contributes to the development of arthritic inflammation. These findings suggest that FcRβ may play a pivotal role in the pathogenesis of arthritic inflammation. To address this possibility, we examined the function of FcRβ in arthritic inflammation employing a mouse model.

Methods

For the induction of arthritis, we injected 2 mg of a cocktail of anti-type II collagen (CII) monoclonal antibodies (mAbs) into C57BL/6J mice (FcRβ+/+) and FcRβ−/− mice intravenously. Three days later, 100 μg lipopolysaccharide (LPS; Escherichia coli 055:B5) was intraperitoneally injected. Joint swelling was evaluated by inspection. Histopathology of joint tissues was examined by hematoxylin and eosin (H&E) or tartrate-resistant acid phosphatase staining.

Results

Here, we demonstrate in a well-established experimental arthritis model induced by LPS and anti-CII mAbs that FcRβ−/− mice exhibit exacerbated arthritic inflammation manifested in paw swelling, leukocyte infiltration into the knee joint, and bone erosion and tissue cytokine expression.

Conclusion

Our findings clearly indicate that FcRβ negatively regulates arthritic inflammation in an experimental arthritis model.

Keywords

Anti-CII mAb-induced arthritis Fc receptor Mast cell 

Notes

Acknowledgments

We thank Toyoharu Jike for his invaluable advice in histological experiments. This work was supported in part by Grants-in-Aid from the Ministry of Education, Culture, Sports, Science, and Technology-Supported Program for the Strategic Research Foundation at Private Universities (to C.R.), Grant-in-Aid for Scientific Research B (KAKENHI22390202 to C.R.), and Young Scientists B (KAKENHI 23791124 to M.O.-Y., 20790699 to S.N.).

Conflict of interest

The authors report no conflict of interest.

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Copyright information

© Japan College of Rheumatology 2012

Authors and Affiliations

  • Mino Ohtsubo-Yoshioka
    • 1
  • Satoshi Nunomura
    • 1
  • Tatsuki R. Kataoka
    • 2
  • Yoshimichi Okayama
    • 1
  • Chisei Ra
    • 1
    Email author
  1. 1.Division of Molecular Cell Immunology and Allergology, Department of Biomedical SciencesNihon University School of MedicineItabashi-kuJapan
  2. 2.Department of Diagnostic PathologyKyoto University HospitalKyotoJapan

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