Journal of Ethology

, Volume 36, Issue 2, pp 135–141 | Cite as

Pre-ovipositional maternal care alleviates food stress of offspring in the flower beetle Dicronocephalus wallichii

  • Wataru Kojima
  • Chung-Ping Lin


Unlike most other flower beetles, females of Dicronocephalus wallichii exhibit nesting behaviour. The female constructs a burrow in the soil, cuts dead plant leaves into small pieces to provision the nest, and then lays one egg inside the nest. Hatched larvae have been thought to feed on the nest materials prepared by their mothers, but the effects of pre-ovipositional care on larval performance have not been tested. The hatched larvae were found to stay in the nest for 15–30 days until they consumed the nest materials. We examined whether the presence of provisioned nests enhanced larval performance under both benign and food-stress conditions. With high-nutrient soil, larval survival rate and growth speed were not affected by the presence of provisioned nests. By contrast, with low-nutrient soil, mortality of the larvae was much higher in the absence than in the presence of provisioned nests. The growth speed of larvae with nests located in low-nutrient soil was as high as those reared in high-nutrient soil. These results indicate that females alleviate the food stress of larvae during their initial developmental stage by constructing provisioned nests.


Flower chafer Nest provisioning Offspring performance Saprophagous insect Scarab beetle Scarabaeidae 



We are very grateful to Dr G. Machado and two anonymous reviewers for valuable comments on the manuscript.


This study was supported by a Japan Society for the Promotion of Science Postdoctoral Fellowship for Research Abroad to Wataru Kojima and research grants of the Ministry of Science and Technology (MOST, 103-2311-B-029-001-MY3 and 104-2621-B-003-002-MY3) to Chung-Ping Lin.

Supplementary material

10164_2018_544_MOESM1_ESM.docx (2.4 mb)
Supplementary material 1 (DOCX 2506 kb)
10164_2018_544_MOESM2_ESM.xlsx (9 kb)
Table S1. Raw data for larval mass and duration of larval staying in the nest (XLSX 8 kb)
10164_2018_544_MOESM3_ESM.xlsx (10 kb)
Table S2. Raw data for larval performance experiment (XLSX 9 kb)


  1. Arce AN, Johnston PR, Smiseth PT, Rozen DE (2012) Mechanisms and fitness effects of antibacterial defences in a carrion beetle. J Evol Biol 25:930–937CrossRefGoogle Scholar
  2. Brandhorst-Hubbard JL, Flanders KL, Appel AG (2001) Oviposition site and food preference of the green June beetle (Coleoptera: Scarabaeidae). J Econ Entomol 94:628–633CrossRefGoogle Scholar
  3. Capodeanu-Nägler A, Keppner EM, Vogel H, Ayasse M, Eggert AK, Sakaluk SK, Steiger S (2016) From facultative to obligatory parental care: interspecific variation in offspring dependency on post-hatching care in burying beetles. Sci Rep 6:29323CrossRefGoogle Scholar
  4. Chaboo CS, Frieiro-Costa FA, Gómez-Zurita J, Westerduijn R (2014) Origins and diversification of subsociality in leaf beetles (Coleoptera: Chrysomelidae: Cassidinae: Chrysomelinae). J Nat Hist 48:2325–2367CrossRefGoogle Scholar
  5. Clutton-Brock TH (1991) The evolution of parental care. Princeton University Press, PrincetonGoogle Scholar
  6. Estes AM, Hearn DJ, Snell-Rood EC, Feindler M, Feeser K, Abebe T, Hotopp JC, Moczek AP (2013) Brood ball-mediated transmission of microbiome members in the dung beetle, Onthophagus taurus (Coleoptera: Scarabaeidae). PLoS One 8:e79061CrossRefGoogle Scholar
  7. Filippi L, Hironaka M, Nomakuchi S, Tojo S (2000) Provisioned Parastrachia japonensis (Hemiptera: Cydnidae) nymphs gain access to food and protection from predators. Anim Behav 60:757–763CrossRefGoogle Scholar
  8. Kasuya E (2001) Mann-Whitney U-test when variances are unequal. Anim Behav 61:1247–1249CrossRefGoogle Scholar
  9. Klug H, Bonsall MB (2014) What are the benefits of parental care? The importance of parental effects on developmental rate. Ecol Evol 4:2330–2351CrossRefGoogle Scholar
  10. Kojima W (2015a) Variation in body size in the giant rhinoceros beetle Trypoxylus dichotomus is mediated by maternal effects on egg size. Ecol Entomol 40:420–427CrossRefGoogle Scholar
  11. Kojima W (2015b) Attraction to carbon dioxide from feeding resources and conspecific neighbours in larvae of the rhinoceros beetle Trypoxylus dichotomus. PLoS One 10:e0141733CrossRefGoogle Scholar
  12. Kojima W, Lin CP (2017) It takes two to tango: functional roles, sexual selection and allometry of multiple male weapons in the flower beetle Dicronocephalus wallichii bourgoini. Biol J Linn Soc 121:514–529CrossRefGoogle Scholar
  13. Kosztolányi A, Nagy N, Kovács T, Barta Z (2015) Predominant female care in the beetle Lethrus apterus with supposedly biparental care. Entomol Sci 18:292–294CrossRefGoogle Scholar
  14. Kramer J, Körner M, Diehl J, Scheiner C, Yüksel-Dadak A, Christl T, Kohlmeier P, Meunier J (2017) When earwig mothers do not care to share: parent-offspring competition and the evolution of family life. Funct Ecol 31:2098–2107CrossRefGoogle Scholar
  15. López-Guerrero Y (1995) Development and histology of the ovary in Cephalodesmius armiger Westwood (Coleoptera: Scarabaeidae, Scarabaeinae). Coleopt Bull 49:332–342Google Scholar
  16. Meunier J, Kölliker M (2012) When it is costly to have a caring mother: food limitation erases the benefits of parental care in earwigs. Biol Lett 8:547–550CrossRefGoogle Scholar
  17. Monteith KM, Andrews C, Smiseth PT (2012) Post-hatching parental care masks the effects of egg size on offspring fitness: a removal experiment on burying beetles. J Evol Biol 25:1815–1822CrossRefGoogle Scholar
  18. Pike DA, Clark RW, Manica A, Tseng HY, Hsu JY, Huang WS (2016) Surf and turf: predation by egg-eating snakes has led to the evolution of parental care in a terrestrial lizard. Sci Rep 6:22207CrossRefGoogle Scholar
  19. Pilakouta N, Jamieson S, Moorad JA, Smiseth PT (2015) Parental care buffers against inbreeding depression in burying beetles. Proc Natl Acad Sci USA 112:8031–8035CrossRefGoogle Scholar
  20. Potter DA (1983) Effect of soil moisture on oviposition, water absorption, and survival of southern masked chafer (Coleoptera: Scarabaeidae) eggs. Environ Entomol 12:1223–1227CrossRefGoogle Scholar
  21. R Development Core Team (2013) R: a language and environment for statistical computing. R Foundation for Statistical Computing, ViennaGoogle Scholar
  22. Royle NJ, Smiseth PT, Kölliker M (2012) The evolution of prental care. Oxford University Press, OxfordGoogle Scholar
  23. Royle NJ, Alonzo SH, Moore AJ (2016) Co-evolution, conflict and complexity: what have we learned about the evolution of parental care behaviours? Curr Opin Behav Sci 12:30–36CrossRefGoogle Scholar
  24. Santos ES, Bueno PP, Gilbert JD, Machado G (2017) Macroecology of parental care in arthropods: higher mortality risk leads to higher benefits of offspring protection in tropical climates. Biol Rev 92:1688–1701CrossRefGoogle Scholar
  25. Schwab DB, Riggs HE, Newton IL, Moczek AP (2016) Developmental and ecological benefits of the maternally transmitted microbiota in a dung beetle. Am Nat 188:679–692CrossRefGoogle Scholar
  26. Šípek P, Král D, Jahn O (2008) Description of the larvae of Dicronocephalus wallichi bourgoini Coleoptera: Scarabaeidae: Cetoniinae) with observations on nesting behavior and life cycle of two Dicronocephalus species under laboratory conditions. Ann Soc Entomol Fr 44:409–417CrossRefGoogle Scholar
  27. Trivers RL (1972) Parental investment and sexual selection. In: Campbell B (ed) Sexual selection and the descent of man. Aldine, Chicago, pp 136–179Google Scholar
  28. Trumbo ST (2017) Feeding upon and preserving a carcass: the function of prehatch parental care in a burying beetle. Anim Behav 130:241–249CrossRefGoogle Scholar
  29. Wenninger EJ, Averill AL (2006) Effects of delayed mating on reproductive output of female oriental beetle Anomala orientalis (Coleoptera: Scarabaeidae). Agric Forest Entomol 8:221–231CrossRefGoogle Scholar

Copyright information

© Japan Ethological Society and Springer Japan KK, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Graduate School of Sciences and Technology for InnovationYamaguchi UniversityYamaguchi CityJapan
  2. 2.Department of Life ScienceNational Taiwan Normal UniversityTaipeiTaiwan

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