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Bioleaching of gold from waste printed circuit boards by Chromobacterium violaceum

  • Jingying LiEmail author
  • Changjin Liang
  • Chuanjing Ma
ORIGINAL ARTICLE

Abstract

Chromobacterium violaceum, a cyanide-generating bacterium has been used to leach out gold from the waste printed circuit boards in culture medium with yeast extract, peptone and glycine. And gold leaching efficiency is affected by many factors, such as dissolved oxygen, base metals, particle size and nutriment, especially several metal ions, which can serve as the catalyst in the metabolism. The dissolved oxygen concentration in every solution decreased to a minimal level after 24 h without oxygen supplement, which was consumed by the bacterial respiration and the reaction of gold and cyanide. pH had a little increase as the generation of OH. In order to supply the oxygen, a homemade oxygenator was used to offer the sterile oxygen for bacterial respiration, and gold leaching efficiency increased drastically. Pretreated by At. ferrooxidans at optimum conditions (selected by orthogonal experiment), above 80 % copper and other base metals can be removed and increased the gold/copper ratio in the residual solid. Bioleaching the biooxidized e-waste significantly improved gold leaching efficiency and 200 mesh is the optimum particle size. The addition of nutritive salts (NaCl and MgSO4·7H2O) strengthened the leaching efficiency, respectively, where the optimum amount was 4 × 10−3 mol/L and 1.7 × 10−1 mol/L, and MgSO4·7H2O enhanced gold leaching efficiency further. All the promoting effect can also be seen from the total cyanide amount generated, which corresponds to the optimum leaching efficiency. The combination of oxygen supplement, nutrients addition and the pretreatment by biooxidation made the gold bioleaching efficiency reach 70.6 %, which was higher than the previous reports.

Keywords

Chromobacterium violaceum Gold bioleaching Oxygen supplement Gold leaching efficiency 

Notes

Acknowledgments

This project was supported by Key Laboratory for Solid Waste Management and Environment Safety, Ministry of Education of China, Tsinghua University: SWMES 2011–02, and supported by Shandong Provincial Natural Science Foundation, China (ZR2013EEM008).

References

  1. 1.
    Heart S (2007) Sustainable management of electronic waste (e-waste). Clean Soil Air Water 35:305–310CrossRefGoogle Scholar
  2. 2.
    United Nations Environment Programme (2012) Urgent need to prepare developing countries for surges in e-waste. http://www.unep.org/Documents.Multilingual/Default.asp?DocumentID=612&ArticleID=6471. Accessed 15 March 2012
  3. 3.
    Townsend TG (2011) Environmental issues and management strategies for waste electronic and electrical equipment. J Air Waste Manag Assoc 61:587–610CrossRefGoogle Scholar
  4. 4.
    Pant D, Joshi D, Upreti MK, Kotnala RK (2012) Chemical and biological extraction of metals present in e-waste: a hybrid technology. Waste Manag 32:979–990CrossRefGoogle Scholar
  5. 5.
    Davis C, Heart S (2008) Electronic waste: the local government perspective in Queensland. Aust Resour Conserv Recycl 52(8–9):1031–1039CrossRefGoogle Scholar
  6. 6.
    Syed S (2012) Recovery of gold from secondary sources-A review. Hydrometallurgy 115–116:30–51CrossRefGoogle Scholar
  7. 7.
    Cui J, Zhang L (2008) Metallurgical recovery of metals from electronic waste: a review. J Hazard Mater 158:228–256CrossRefGoogle Scholar
  8. 8.
    Pradhan JK, Kumar S (2012) Metals bioleaching from electronic waste by Chromobacterium violaceum and Pseudomonads sp. Waste Manag Res 30(11):1151–1159CrossRefGoogle Scholar
  9. 9.
    Brandl H, Lehmann S, Faramarzi MA (2006) Microbe-metal-interactions for the biotechnological treatment of metal-containing solid waste. China Part 4(2):93–97CrossRefGoogle Scholar
  10. 10.
    Brandl H, Lehmann S, Faramarzi MA, Martinelli D (2008) Biomobilization of silver, gold, and platinum from solid waste materials by HCN-forming microorganisms. Hydrometallurgy 94:14–17CrossRefGoogle Scholar
  11. 11.
    Chi DT, Lee JC, Pandey BD, Jeong J, Yoo K, Huynh TH (2011) Bacterial cyanide generation in the presence of metal ions (Na+, Mg2+, Fe2+, Pb2+) and gold bioleaching from waste PCBs. J Chem Eng Jpn 44(10):692–700CrossRefGoogle Scholar
  12. 12.
    Knowles CJ, Bunch AW (1986) Microbial cyanide metabolism. Adv Microb Physiol 27:74–111Google Scholar
  13. 13.
    Creczynski-Pasa TB, Antônio RV (2004) Energetic metabolism of Chromobacterium violaceum. Genet Mol Res GMR 3:162–166Google Scholar
  14. 14.
    Pham VA, Ting YP (2009) Gold bioleaching of electronic waste by cyanogenic bacteria and its enhancement with bio-oxidation. Adv Mater Res 71–73:661–664CrossRefGoogle Scholar
  15. 15.
    Chi DT, Lee JC, Pandey BD, Yoo K, Jeong J (2011) Bioleaching of gold and copper from waste mobile phone PCBs by using a cyanogenic bacterium. Miner Eng 24:1219–1222CrossRefGoogle Scholar
  16. 16.
    Urán MD, Aljoni-Alario AF, Urán ND (2010) Chromobacterium violaceum and its important metabolites-review. Folia Microbiol 55(6):535–547CrossRefGoogle Scholar
  17. 17.
    Kita Y, Nishikawa H, Ike M, Takemoto T (2005) Low environmentally impact recovery of gold using cyanide producing bacteria. In: Fourth international symposium on environmentally conscious design and inverse manufacturing, 2005. Eco Design 2005, IEEE, pp 935–938Google Scholar
  18. 18.
    Kita Y, Nishikawa H, Ike M, Takemoto T (2008) Enhancement of Au dissolution by microorganisms using an accelerating cathode reaction. Metall Mater Trans B Process Metall Mater Process Sci 40(1):39–44CrossRefGoogle Scholar
  19. 19.
    Anjum F, Shahid M, Akcil A (2012) Biohydrometallurgy techniques of low grade ores: a review on black shale. Hydrometallurgy 117–118:1–12CrossRefGoogle Scholar
  20. 20.
    Choi MS, Cho KS, Kim DS, Kim DJ (2004) Microbial recovery of copper from printed circuit boards of waste computer by Acidithiobacillus ferrooxidans. J Environ Sci Health Part A Tox Hazard Subst Environ Eng 39(11–12):2973–2982CrossRefGoogle Scholar
  21. 21.
    Wang JW, Bai JF, Xu JQ, Liang B (2009) Bioleaching of metals from printed wire boards by Acidithiobacillus ferrooxidans and Acidithiobacillus thiooxidans and their mixture. J Hazard Mater 172(2–3):1100–1105CrossRefGoogle Scholar
  22. 22.
    Logsdon MJ, Hagelstein K, Mudder TI (1999) The management of cyanide in gold extraction. ICME Publications, OttawaGoogle Scholar
  23. 23.
    Foucher S, Battaglia-Brunet F, d’Hugues P, Clarens M, Godon JJ, Morin D (2003) Evolution of the bacterial population during the batch bioleaching of a cobaltiferous pyriteina suspended-solids bubble column and comparison with a mechanically agitated reactor. Hydrometallurgy 71(1–2):5–12CrossRefGoogle Scholar
  24. 24.
    Kita Y, Nishikawa H, Takemoto T (2006) Effects of cyanide and dissolved oxygen concentration on biological Au recovery. J Biotechnol 124:545–551CrossRefGoogle Scholar
  25. 25.
    Sand W, Gehrke T, Jozsa PG, Schippers A (2001) (Bio)chemistry of bacterial leaching-direct vs. indirect bioleaching. Hydrometallurgy 59:159–175CrossRefGoogle Scholar
  26. 26.
    Wadsworth ME, Zhu X, Thompson JS, Pereira CJ (2000) Gold dissolution and activation in cyanide solution: kinetics and mechanism. Hydrometallurgy 57:1–11CrossRefGoogle Scholar
  27. 27.
    Lawson EN, Barkhuizen M, Dew DW (1999) Gold Solubilisation by the cyanide producing bacteria Chromobacterium violaceum. In: Proceedings of International biohydrometallurgy Symposium, biohydrometallurgy and the environment towards the mining of 21st century, Process Metallurgy. 9, Part 1. Madrid, Spain, pp 239–246Google Scholar
  28. 28.
    Akcil A, Karahan AG, Ciftci H, Sagdic O (2003) Biological treatment of cyanide by natural isolated bacteria (Pseudomonas sp.). Miner Eng 16:643–649CrossRefGoogle Scholar
  29. 29.
    Faramarzi MA, Brandl H (2006) Formation of water-soluble metal cyanide complexes from solid minerals by Pseudomonas plecoglossicida. FEMS Microbiol Lett 259:47–52CrossRefGoogle Scholar
  30. 30.
    Watling HR (2006) The bioleaching of sulphide minerals with emphasis on copper sulphides —a review. Hydrometallurgy 84:81–108CrossRefGoogle Scholar
  31. 31.
    Rawlings DE (1998) Industrial practice and the biology of leaching of metals from ores: The 1997 Pan Labs Lecture. J Ind Microbiol Biotechnol 20:268–274CrossRefGoogle Scholar

Copyright information

© Springer Japan 2014

Authors and Affiliations

  1. 1.College of Environment and Safety EngineeringQingdao University of Science and TechnologyQingdaoChina
  2. 2.Hanshan Normal UniversityChaozhouChina

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