Noise-Induced Hypersensitization of the Acoustic Startle Response in Larval Zebrafish
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Overexposure to loud noise is known to lead to deficits in auditory sensitivity and perception. We studied the effects of noise exposure on sensorimotor behaviors of larval (5–7 days post-fertilization) zebrafish (Danio rerio), particularly the auditory-evoked startle response and hearing sensitivity to acoustic startle stimuli. We observed a temporary 10–15 dB decrease in startle response threshold after 18 h of flat-spectrum noise exposure at 20 dB re·1 ms−2. Larval zebrafish also exhibited decreased habituation to startle-inducing stimuli following noise exposure. The noise-induced sensitization was not due to changes in absolute hearing thresholds, but was specific to the auditory-evoked escape responses. The observed noise-induced sensitization was disrupted by AMPA receptor blockade using DNQX, but not NMDA receptor blockade. Together, these experiments suggest a complex effect of noise exposure on the neural circuits mediating auditory-evoked behaviors in larval zebrafish.
KEYWORDSinner ear damage behavior hearing prepulse inhibition
We would like to thank Dr. Allison Coffin and Dr. David Glanzman for advice and Tor Linbo and Dr. Dale Hailey for help with training.
This work was supported by the University of Washington Auditory Neuroscience training grant [NIH 2T32DC005361-11] to AAB.
- Bhandiwad AA, Sisneros JA (2016) Revisiting psychoacoustic methods for the assessment of fish hearing. In: J Sisneros (ed) Fish hearing and bioacoustics. Adv Exp Med Biol 877, Springer, Cham, pp 157–184Google Scholar
- Dinh CT, Goncalves S, Bas E, Van De Water TR, Zine A (2016) Molecular regulation of auditory hair cell death and approaches to protect sensory receptory cells and/or stimulate repair following acoustic trauma. Front Cell Neurosci 9:96Google Scholar
- Eggermont JJ (2015) Animal models of spontaneous activity in the healthy and impaired auditory system. Front Neur Circuit 9:19Google Scholar
- Finneran JJ (2012) Auditory effects of underwater noise in odontocetes. In: The effects of noise on aquatic life. Springer, New York, pp 197–202Google Scholar
- Melnick W (1976). Human asymptotic threshold shift. Effects of noise on hearing. pp 277–289Google Scholar
- Olt J, Johnson SL, Marcotti W (2014) In vivo and in vitro biophysical properties of hair cells from the lateral line and inner ear of developing and adult zebrafish. J Phys 592(10):2041–2058Google Scholar
- Pickles JO (2012) An introduction to the physiology of hearing, 4th edn. Emerald Group Publishing, BingleyGoogle Scholar
- Smith ME (2012). Predicting hearing loss in fishes. In The effects of noise on aquatic life. Springer New York, pp 259–262Google Scholar
- Westerfield M (2000) The zebrafish book: a guide for the laboratory use of zebrafish (Danio rerio), 4th edn. Univ. of Oregon Press, EugeneGoogle Scholar
- Yang G, Lobarinas F, Zhang L, Turner J, Stolzberg D, Salvi R, Sun W (2007) Salicylate induced tinnitus: behavioral measures and neural activity in auditory cortex of awake rats. Hear Res 226:244–253Google Scholar