Endothelial Cell, Pericyte, and Perivascular Resident Macrophage-Type Melanocyte Interactions Regulate Cochlear Intrastrial Fluid–Blood Barrier Permeability
- 2.3k Downloads
The integrity of the fluid–blood barrier in the stria vascularis is critical for maintaining inner ear homeostasis, especially for sustaining the endocochlear potential, an essential driving force for hearing function. However, the mechanisms that control intrastrial fluid–blood barrier permeability remain largely unknown. At the cellular level, the intrastrial fluid–blood barrier comprises cochlear microvascular endothelial cells connected to each other by tight junctions (TJs), an underlying basement membrane, and a second line of support consisting of cochlear pericytes and perivascular resident macrophage-type melanocytes. In this study, we use a newly established primary cell culture-based in vitro model to show that endothelial cells, pericytes, and perivascular resident macrophage-type melanocytes interact to control intrastrial fluid–blood barrier permeability. When the endothelial cell monolayer was treated with pericyte—or perivascular resident macrophage-type melanocyte—conditioned media, the permeability of the endothelial cell monolayer was significantly reduced relative to an untreated endothelial cell monolayer. Further study has shown the pericytes and perivascular resident macrophage-type melanocytes to regulate TJ expression in the endothelial cell monolayer. The new cell culture-based in vitro model offers a unique opportunity to obtain information on the organ-specific characteristics of the cochlear blood/tissue barrier. Our finding demonstrates the importance of signaling among pericytes, endothelial cells, and perivascular resident macrophage-type melanocytes to the integrity of the intrastrial fluid–blood barrier.
Keywordsendothelial cell pericyte perivascular resident macrophages-type melanocyte primary cell line paracellular permeability mouse cochlea
Integrity of the intrastrial fluid–blood barrier is critical for solute homeostasis and prevention of the influx of toxic substances (Juhn and Rybak 1981; Juhn et al. 2001).1 Loss of intrastrial fluid–blood barrier integrity, with concomitant vascular permeability, results in cochlear edema in the stria vascularis. Cochlear edema is thought to be present in a number of hearing disorders, including autoimmune inner ear disease, noise-induced hearing loss, and several genetically linked diseases (McMenomey et al. 1992; Cohen-Salmon et al. 2007; Klein et al. 2008; Kellerhals 1972; Hukee and Duvall 1985; Suzuki et al. 2002; Shi 2009). Recent progress in understanding the physiology of the blood–brain barrier (BBB) and blood–retinal barrier (BRB) provides useful insight into the largely unknown and poorly understood mechanisms that control endothelial blood–barrier permeability in the stria vascularis. Gaps in our understanding of the intrastrial fluid–blood barrier significantly limit development of effective drug therapies for vascular dysfunction-related hearing loss. Targeting dysfunctions, which involve maintenance of the endolymphatic potential, is particularly important.
Pericyte-endothelial interactions, known to be a particularly salient feature of the blood–brain barrier, profoundly affect basement membrane and endothelial tight junction structure and function (Liu et al. 2012). In an earlier study, we showed pericytes to densely populate the surface of capillaries in the stria vascularis (Shi et al. 2008). However, the role of pericytes in vascular stability has not been determined.
In this study, we used a new cell culture-based in vitro model to directly study the signaling between endothelial cells, pericytes, and perivascular resident macrophage-type melanocytes. The aim is to determine the specific contribution of each phenotype to intrastrial fluid–blood barrier integrity and obtain information on the organ-specific characteristics of the cochlear blood/tissue barrier.
Materials and methods
C57BL/6J (aged 10–15 days) mice were obtained by the use of inbred C57BL/6J mice purchased from Jackson Laboratory (Bar Harbor, ME, USA). The protocol for the care and use of these animals was approved by the Institutional Animal Care and Use Committee at Oregon Health & Science University (IACUC approval number B11265).
Primary cell culture
Protocols for generating the three primary cell lines used in these experiments were previously reported (Zhang et al. 2012). Briefly, cochleae from 10- to 15-day-old C57BL/6J mice were harvested under sterile conditions. The stria vascularis was gently pulled away from the spiral ligament and placed in ice-cold perilymph solution containing 125 mmol/L NaCl, 3.5 mmol/L KCl, 1.3 mmol/L CaCl2, 1.5 mmol/L MgCl2, 0.51 mmol/L NaH2PO4, 10 mmol/L HEPES, and 5 mmol/L glucose at pH 7.4 with osmolarity adjusted to 310 mmol/kg. The isolated stria vascularis was washed with gentle shaking in cool perilymph solution for 10 min and transferred to a clean 35-mm collagen I coated dish with 2 ml of cell culture medium. The stria vascularis was cut into small pieces with sterile ophthalmic tweezers under a dissection microscope. Fragmented pieces of the stria vascularis were seeded around the dish to roughly uniform density. The procedure for situating the fragments requires approximately 2 h. The medium is changed every 3 days. The cells are incubated at 37 ° in 5 % CO2 for 7–14 days until the cell clones melt. Cell clusters of each phenotype are visible by phase contrast microscopy at 6–7 days, at which time the cells are passaged into a 60-mm collagen I coated dish. Pericytes were cultured in Dulbecco’s modified Eagle’s medium (DMEM low glucose, Invitrogen, USA) containing 10 % fetal bovine serum (FBS, Sigma, USA), 100 nM pigment epithelium-derived factor (PEDF), and 1 % penicillin/streptomycin solution (10,000 U penicillin and 10 mg streptomycin/ml, Sigma, USA). Endothelial cells were cultured in CS-C medium (Sigma, USA) containing 10 % fetal bovine serum (FBS, Sigma, USA), 1 % endothelial cell growth factor (Sigma, USA), and 1 % penicillin/streptomycin solution (10,000 U penicillin and 10 mg streptomycin/ml, Sigma, USA), and perivascular resident macrophages in medium 254CF (Invitrogen, USA) containing 1 % human melanocyte growth supplement (Invitrogen, USA), 10 % FBS (Sigma, USA), and 0.2 % gentamicin/amphotericin B solution (Sigma, USA).
Cell purification with flow cytometry
The cultured cells were purified by fluorescence-activated cell sorting with a BD influx cell sorter operated at 45 psi using a 70-μm nozzle. First, the cultured cells were detached from the dish with a 0.25 % trypsin-EDTA solution (Sigma, USA). The cells were gently washed in 2 ml of their culture medium, placed in a 12 × 75 mm polypropylene FACS tube, and centrifuged (300×g, 5 min, 4 °C). Cells (1 × 106 cells/ml) were incubated with marker antibodies for 30 min in the dark at 4 °C, followed by 2 ml of their cultured medium to wash the cells, and centrifuged (300×g, 5 min, 4 °C). After discarding the supernatant, the cells were resuspended in culture medium to a concentration of 1 × 106 cells/ml. Phycoerythrin-conjugated rat monoclonal antibody labeling for platelet-derived growth factor beta receptor (Abcam, USA) at a concentration of 0.5 μg/ml was used to identity pericytes in sorting. Rat IgG phycoerythrin (Abcam, USA) at a concentration of 0.2 μg/ml served as the isotype control label. The endothelial cells were identified with fluorescein isothiocyanate (FITC)-conjugated isolectin B4 (BSI-B4) (Sigma, USA) at a concentration of 20 μg/ml, with cells in 0.2 M galactose (Sigma, USA) preincubated with lectin serving as the control. Perivascular resident macrophage-type melanocytes were identified with a phycoerythrin-conjugated rat monoclonal antibody for F4/80 (Abcam, USA) at a concentration of 10 μl/106 cells (in 100 μl), with cells labeled by rat IgG phycoerythrin (Abcam, USA) at a concentration of 0.2 μg/ml serving as the isotype control. Positive cells from sorting were collected in culture media and plated on 60 mm collagen I coated dishes.
Sorted cells were validated with antibodies for proteins that detected marker genes from the sorted cell DNA. In this study, aliquots of each cell phenotype were grown on 35-mm collagen-coated glass bottom dishes and labeled for immunohistochemical confirmation of specific markers including anti-von Willebrand factor (vWF) for endothelial cells, anti-F4/80 and anti-GSTα4 for perivascular resident macrophages, and anti-desmin and anti-NG2 for pericytes.
Immunohistochemistry and fluorescence microscopy
Primary antibodies employed
1:50 (dilution with 1 % BSA-PBS)
Rabbit polyclonal antibody to VE Cadherin
Reacts with Ms, Ch, Hu
1:50 (dilution with 1 % BSA–PBS)
Rabbit polyclonal antibody to ZO-1
Reacts with Ms, Hu, Rat, Gp
1:50 (dilution with 1 % BSA-PBS)
Rat monoclonal antibody to F4/80
Reacts with Ms
1;100 (dilution with 1 % BSA-PBS)
Goat polyclonal antibody to GSTα4
Reacts with Ms, Hu
von Willebrand Factor
1:100 (dilution with 1 % BSA-PBS)
Sheep polyclonal to von Willebrand factor
Reacts with Ms, Hu, Pig
1:100 (dilution with 1 % BSA-PBS)
Rabbit monoclonal to desmin
Reacts with Ms, Rat, Gp, Hu
1:100 (dilution with 1 % BSA-PBS)
Mouse monoclonal to NG2
Reacts with Ms, Hu
To visualize the morphology of the intrastrial fluid–blood barrier, we triple-labeled lateral-wall tissues, adding antibody for desmin to identify pericytes (Abcam) and antibody for F4/80 to identify perivascular resident macrophage-like melanocytes (eBioscience), to cochlear capillary endothelial cells prelabeled with the fluorescent dye 1,1-dioctadecyl-3,3,3,3-tetramethylindocar bocyanineperchlorate, Dil (Dai and Shi 2011). The procedure for immunohistochemical labeling of desmin and F/80 was the same as described above.
Characterization of the endothelial cell monolayer
Confluent monolayers of endothelial cells are characterized by low endothelial monolayer permeability to FITC-dextran and by expression of tight junction proteins such as ZO-1 and VE-cadherin (Zhang et al. 2012).
Production of conditioned media
DMEM (Life Technologies, USA) containing 10 % FBS (Sigma, USA) and 1 % penicillin–streptomycin solution (10,000 U of penicillin and 10 mg streptomycin/ml) (Sigma, USA) was used as a starter general culture medium (CM). To obtain pericyte or perivascular resident macrophage-type melanocyte conditioned media, 5 × 105 pericytes or 5 × 105 perivascular resident macrophage-type melanocytes were cultured in a 60 mm cell culture dish with CM at 37 °C in a 5 % CO2 incubator. After 3 days of cultivation, the supernatant of the culture was decanted. Debris was removed by centrifugation at 550×g for 5 min. The supernatants (conditioned media) were aliquoted and stored at −20 °C until use.
Cell culture for the coculture model
Five different culture models (one control model and four experimental models) were developed in this study. For the control group, purified endothelial cells at passage 3 at a density of 4 × 104/cm2 were seeded and grown on the polyethylene terephthalate membranes of Transwell inserts (BD Biosciences, catalog number 353096) overnight. In all cases, endothelial cells monolayers were not formed by overnight growth. All experimental groups were seeded with endothelial cells at the same passage number and density (4 × 104/cm2) and grown on the inserts for 5 days. Treatment in experimental model 1 was with 4 ml CM, experimental model II with 2 ml CM and 2 ml PC conditioned medium, experimental model III with 2 ml CM and 2 ml perivascular resident macrophage-type melanocyte conditioned medium, and experimental model IV with 2 ml pericyte conditioned medium and 2 ml perivascular resident macrophage-type melanocyte conditioned medium.
Assessment of endothelial monolayer permeability
Transendothelial permeability of the endothelial cell monolayer to FITC-dextran (MW, 70 k Da) was measured according to the manufacturer's instructions (catalog number ECM644, vascular permeability assay kit; Millipore, Billerica, MA, USA) and described in our previous study (Zhang et al. 2012). Briefly, the small fluorescent molecule (diluted at 1:40 with medium) permeates through the monolayer into the basolateral chamber. The amount of dextran accumulating in the basolateral chamber is an indicator of the permeability of the endothelial cell layer. Permeability of the endothelial cell monolayer was assessed as FITC-dextran fluorescence with a multiwell microplate reader (TECAN GENios ELISA reader, Tecan Group Ltd.) at excitation/emission wavelengths of 485/535 nm.
All statistical analyses were performed using GraphPad Prism 5.0. Data, presented as means ± SD, were evaluated using one-way ANOVA followed by a Tukey’s multiple comparison test (α level = 0.05 in all cases) for comparisons of three or more groups.
Morphology of the intrastrial fluid–blood barrier
Production and validation of the barrier component cell lines
Verification of a functional endothelial monolayer
Pericyte and perivascular resident macrophage-type melanocyte secretions affect the integrity of intrastrial fluid–blood barrier
Pericyte and perivascular resident macrophage signaling affects expression of TJ proteins in the intrastrial fluid–blood barrier
Over the past few decades, in vitro cell-based models, widely used in BBB and BRB studies, have been powerful tools for investigating cell–cell interactions (Lamm et al. 1994; Rauch and Reale 1968; Shi 2010). Several different methods for isolating and culturing cells from the adrenal gland, umbilical vein, lung, skeletal muscle, and brain have been reported (Juhn et al. 1982; Sakagami et al. 1982, 1986, 1987; Juhn et al. 2001; Tagaya et al. 2011; Laurell et al. 2008). However, these methods have not been suitable for isolating intrastrial fluid–blood barrier cells. The methods used for capillary extraction and isolation on large tissue volumes have not been suitable for production of barrier component cells from the small volume, anatomically complex tissues of the inner ear. Our limited knowledge of the physiology of the cochlear blood barrier is partly the result of not having primary endothelial cells, pericytes, and perivascular resident macrophage-type melanocytes from the inner ear on which to experiment.
The signaling interactions between endothelial cells, pericytes, and perivascular resident macrophage-type melanocytes were investigated with in vitro models utilizing primary cell types. In particular, coculture of the cell types in various conformations of in vitro models provides the means to parse the signaling and identify the unique pericyte and perivascular resident macrophage-type melanocyte contribution to endothelial cell monolayer permeability.
In this study, using primary cell lines and coculture models, we found that strial pericytes secrete substances into the culture medium, which affect barrier properties, and subsequent treatment with pericyte secretion augmented medium “tightens” endothelial cell monolayer integrity (Fig. 5C). Consistent with our previous report (Zhang et al. 2012), signals from perivascular resident macrophage-type melanocyte secretion augmented culture media also affect endothelial barrier permeability in vitro.
TJs between adjacent endothelial cells physiologically explain the limited paracellular permeability of the intrastrial fluid–blood barrier. The permeability properties of the barrier reflect, to a major degree, the tightness of the intercellular junction. The major TJ proteins in the intrastrial fluid–blood barrier are occludin, claudins, zona occludens (ZOs), and adherens junction proteins (Kitajiri et al. 2004a; Yang et al. 2011). The TJ proteins are connected to the actin cytoskeleton by accessory/anchoring proteins. In this study, we examined the TJ protein ZO-1, an important adaptor protein linking transmembrane junctional complexes to the underlying actin cytoskeleton, and VE-cadherin, an important adhesive protein known to tighten the intrastrial fluid–blood barrier.
Pericyte and perivascular resident macrophage-type melanocyte conditioned culture media increased expression of TJ proteins such as ZO-1 and VE-cadherin in the endothelial cell monolayer at both the mRNA transcript and protein levels (Fig. 6). The data indicate secreted signals from pericytes and perivascular resident macrophage-type melanocytes cause increased expression of TJs, which accounts for the change in endothelial barrier permeability.
Our previous study showed the effect of perivascular resident macrophage-type melanocytes on intrastrial fluid–blood barrier integrity mediated by PEDF (Zhang et al. 2012). PEDF was shown to modulate expression of TJ-associated proteins. We presume that the effective agent responsible for the upregulation of TJs by perivascular resident macrophage-type melanocyte conditioned medium is largely the secreted PEDF. However, other unknown signal molecules may also be contributing to TJ expression between endothelial cells.
The agent secreted by pericytes into the conditioned medium and responsible for the effect on TJ expression in endothelial cells is unknown. Also to be determined are interaction effects between the secretions of pericytes and perivascular resident macrophage-type melanocytes. Endothelial cell monolayers treated with a mixture of pericyte + perivascular resident macrophage-type melanocyte conditioned culture medium showed statistical differences in permeability relative to nontreated endothelial cell monolayers; however, no significant difference in endothelial cell monolayer permeability was found between pericyte + perivascular resident macrophage-type melanocyte conditioned culture medium treatment and separate treatment with either pericyte or perivascular resident macrophage-type melanocyte conditioned culture medium.
The intrastrial fluid–blood barrier in the cochlea is a dynamic interface that regulates the influx and efflux of substances, playing an important role in stabilizing the cochlear intrastrial space and sustaining the endocochlear potential. Many intrastrial fluid–blood barrier dysfunction-related hemostatic imbalances have unknown causes. Is altered signaling between barrier component cells the root cause for some of cochlear hemostatic imbalance? How do pathological conditions such as ischemia, hypoxia, and inflammation in the cochlea affect cell–cell interactions? Knowledge of these interactions will facilitate development of new protective and restorative therapies for intrastrial fluid–blood barrier dysfunction-related hearing disorders that involve aberrant cell–cell signaling.
Taken together, our newly established in vitro coculture model provides the first evidence that cochlear pericytes and perivascular resident macrophage-type melanocytes have an important role in maintaining barrier integrity. Our findings show pericytes in the cochlea to contribute to barrier function in the stria vascularis similar to the way pericytes contribute to barrier function in the brain (Armulik et al. 2010) and retina (Kim et al. 2009). As in the brain and retina, absence of pericyte secretory signals increases endothelial cell permeability. A similar proximal mechanism is also found in the ear. Pericyte signaling to endothelial cells upregulates ZO-1 and VE-cadherin. The study confirms the essential cosignaling role of perivascular resident macrophage-type melanocytes to barrier integrity.
We thank Ahmed Hassan and John Henry at life Sciences Division, Lawrence Berkeley National Laboratory, Berkeley for their input and guidance in developing workflows for 3D rendering. This work was supported by National Institutes of Health/National Institute on Deafness and Other Communication Disorders Grants DC008888-02A1 (to X.S.), DC008888-02S1 (to X.S.), R01-DC010844 (to X.S.), NIH NIDCD R21-DC12398-01 (to X.R), and NIHP30-DC005983.
- Dai M, Yang Y, Omelchenko I, Nuttall AL, Kachelmeier A, Xiu R, Shi X (2010) Bone marrow cell recruitment mediated by inducible nitric oxide synthase/stromal cell-derived factor-1alpha signaling repairs the acoustically damaged cochlear blood-labyrinth barrier. Am J Pathol 177(6):3089–3099PubMedCrossRefGoogle Scholar
- Kitajiri S, Miyamoto T, Mineharu A, Sonoda N, Furuse K, Hata M, Sasaki H, Mori Y, Kubota T, Ito J, Furuse M, Tsukita S (2004a) Compartmentalization established by claudin-11-based tight junctions in stria vascularis is required for hearing through generation of endocochlear potential. J Cell Sci 117(Pt 21):5087–5096PubMedCrossRefGoogle Scholar
- Sakagami M, Harada T, Sano M, Sakai S, Matsunaga T (1987) Quantitative evaluation of pinocytosis of capillaries of the stria vascularis under normal and experimental conditions. Acta Otolaryngol 103(3–4):189–197Google Scholar
- Yang Y, Dai M, Wilson TM, Omelchenko I, Klimek JE, Wilmarth PA, David LL, Nuttall AL, Gillespie PG, Shi X (2011) Na+/K+−ATPase alpha1 identified as an abundant protein in the blood–labyrinth barrier that plays an essential role in the barrier integrity. PLoS One 6(1):e16547PubMedCrossRefGoogle Scholar
- Zhang W, Dai M, Fridberger A, Hassan A, Degagne J, Neng L, Zhang F, He W, Ren T, Trune D, Auer M, Shi X (2012) Perivascular-resident macrophage-like melanocytes in the inner ear are essential for the integrity of the intrastrial fluid–blood barrier. Proc Natl Acad Sci U S A 109(26):10388–10393PubMedCrossRefGoogle Scholar
Open Access This article is distributed under the terms of the Creative Commons Attribution License which permits any use, distribution, and reproduction in any medium, provided the original author(s) and the source are credited.