Glycosylation Regulates Prestin Cellular Activity
Glycosylation is a common post-translational modification of proteins and is implicated in a variety of cellular functions including protein folding, degradation, sorting and trafficking, and membrane protein recycling. The membrane protein prestin is an essential component of the membrane-based motor driving electromotility changes (electromotility) in the outer hair cell (OHC), a central process in auditory transduction. Prestin was earlier identified to possess two N-glycosylation sites (N163, N166) that, when mutated, marginally affect prestin nonlinear capacitance (NLC) function in cultured cells. Here, we show that the double mutant prestinNN163/166AA is not glycosylated and shows the expected NLC properties in the untreated and cholesterol-depleted HEK 293 cell model. In addition, unlike WT prestin that readily forms oligomers, prestinNN163/166AA is enriched as monomers and more mobile in the plasma membrane, suggesting that oligomerization of prestin is dependent on glycosylation but is not essential for the generation of NLC in HEK 293 cells. However, in the presence of increased membrane cholesterol, unlike the hyperpolarizing shift in NLC seen with WT prestin, cells expressing prestinNN163/166AA exhibit a linear capacitance function. In an attempt to explain this finding, we discovered that both WT prestin and prestinNN163/166AA participate in cholesterol-dependent cellular trafficking. In contrast to WT prestin, prestinNN163/166AA shows a significant cholesterol-dependent decrease in cell-surface expression, which may explain the loss of NLC function. Based on our observations, we conclude that glycosylation regulates self-association and cellular trafficking of prestinNN163/166AA. These observations are the first to implicate a regulatory role for cellular trafficking and sorting in prestin function. We speculate that the cholesterol regulation of prestin occurs through localization to and internalization from membrane microdomains by clathrin- and caveolin-dependent mechanisms.
Keywordstrafficking membrane glycosylation nonlinear capacitance
Prestin is an integral membrane protein found in outer hair cells (OHCs) of the cochlea (Zheng et al. 2000) and forms an essential component of a molecular motor based in the lateral wall of OHCs (Belyantseva et al. 2000; Liberman et al. 2002; Adler et al. 2003; Yu et al. 2006). In conjunction with the OHC membrane, prestin responds to changes in electrical potential across the membrane to elicit mechanical motion, a phenomenon called electromotility (Brownell et al. 1985; Zheng et al. 2000; Brownell 2006; Ashmore 2008). This is accompanied by a bell-shaped nonlinear capacitance (NLC) function, resulting from charge movement within, as opposed to through, the membrane (Ludwig et al. 2001; Oliver et al. 2001). This NLC is widely regarded as the electrical signature of electromotility and provides an effective readout of prestin function.
The lateral membrane of the OHC is a highly specialized structure adapted for electromechanical transduction at frequencies approaching 100 kHz (Frank et al. 1999; Brownell et al. 2001; Brownell 2006; Ashmore 2008). The lateral wall of the adult OHC is low in cholesterol (Santi et al. 1994; Nguyen and Brownell 1998; Oghalai et al. 1999; Brownell and Oghalai 2000; Rajagopalan et al. 2007). This may have an impact on the modulation of prestin function, as recent observations indicate a direct and dynamic link between membrane cholesterol levels and prestin's functional and biochemical properties (Sturm et al. 2006; Rajagopalan et al. 2007).
Matsuda et al. (2004) showed that prestin is glycosylated in several cell lines and OHCs and identified residues N163 and N166 to be glycosylated. However, no role of glycosylation in prestin structure or function was demonstrated, the only obvious consequence of nonglycosylation being a small shift in voltage at peak capacitance (Vpkc; Matsuda et al. 2004). N-glycosylation of proteins is an important post-translational covalent modification that has been implicated in protein folding, sorting and trafficking, and importantly, membrane and raft localization of proteins (Huet et al. 2003; Fullekrug and Simons 2004). Based on the propensity of prestin to localize to cholesterol-rich membrane microdomains (Sturm et al. 2006; Rajagopalan et al. 2007), we investigated the possible role of glycosylation in membrane raft localization and trafficking of prestin. Our results indicate a functional and regulatory role for glycosylation in the cellular trafficking and/or internalization, particularly cholesterol-induced endocytosis, of prestin.
Antiflotillin-1 antibody (1:250) was purchased from BD Biosciences (San Jose, CA, USA), anti-HA (1:1,000) was purchased from Cell Signaling Technology (Danvers, MA, USA), and AlexaFluor 594 goat antimouse antibody (1:800) and concanavalin A–AlexaFluor 350 conjugate (working concentration 50–200 µg/ml) were purchased from Molecular Probes (Carlsbad, CA, USA). Peroxidase-labeled horse antimouse antibody was obtained from Vector Laboratories (Burlingame, CA, USA). Methyl-β-cyclodextrin (MβCD), water-soluble cholesterol, and bovine serum albumin were obtained from Sigma (St. Louis, MO, USA). The ECL western blotting detection kit was obtained from Amersham (Piscataway, NJ, USA). N-glycopeptidase-F (PNGase-F) was obtained from New England Biolabs (Ipswich, MA, USA). Primers were obtained from Sigma Genosys.
Cloning and mutagenesis
Gerbil prestin was cloned into the pIRES-GFP vector as a HA-tagged fusion protein (HA-prestin), as previously described (Rajagopalan et al. 2006; Sturm et al. 2006). Gerbil prestin was also cloned into the pEGFP-N1 vector as a fusion protein with GFP, as previously described (Sturm et al. 2006). A mutation A206K was engineered into the GFP sequence in the prestin-GFP plasmid to render GFP predominantly monomeric; this construct is referred to as prestin-mGFP. The NN163/166AA mutant (prestinNN163/166AA) was created in both the HA-prestin and the prestin-mGFP constructs using the Quikchange site-directed mutagenesis system (Stratagene, La Jolla, CA, USA) with the following primers (mutated codons are italicized): forward primer 5′GAGGAGTGGCCGCAACCGCCGGCACGGAG3′; reverse primer 5′CCTCCGTGCCGGCGGTTGCGGCCACTCCTC3′. The sequence of prestinNN163/166AA was verified using five overlapping sequencing primers. Cav1-mRFP was obtained from Dr. Ari Helenius, ETH Zurich through Addgene. The mRFP-clathrin construct was obtained from Dr. Pietro DeCamilli, HHMI, Yale University.
Cell culture and transfection
HEK 293 cell lines were maintained with Dulbecco's modified Eagle's medium with 10% FBS and 1% streptomycin and penicillin. Twenty-four hours after passage or at ∼50% confluence, cells were transfected with WT HA-prestin or prestin-mGFP or with HA-prestinNN163/166AA or prestinNN163/166AA-mGFP at a 3:1 ratio with FuGene 6 (Roche, Indianapolis, IN, USA) or cotransfected (1:1 ratio) with either Cav1-mRFP or mRFP-clathrin and either WT prestin-mGFP or prestinNN163/166AA-mGFP at a 3:1 ratio of total DNA to FuGene 6.
HEK 293 cells transfected with either WT HA-prestin or HA-prestinNN163/166AA were harvested 48 h post-transfection and lysed in buffer (200 mM NaCl, 10 mM ethylenediaminetetraacetic acid [EDTA], and 50 mM 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid [HEPES], pH 7.4 and protease inhibitor cocktail), homogenized, and clarified by centrifugation at 1,000×g for 10 min. Supernatants were pelleted at 110,000×g for 40 min. Pellets were solubilized (50 mM Tris–HCl, pH 8.0, 1% sodium dodecyl sulfate) and denatured in glycoprotein-denaturing buffer (New England Biolabs, Ipswich, MA, USA) at 100°C for 10 min before incubating with N-glycopeptidase-F (PNGase-F) for 1 h at 37°C. Proteins were separated on 10% sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and transferred to a nitrocellulose membrane. The membranes were blocked with 5% dry milk in phosphate-buffered saline with 1% Triton-X100 (PBST). The membrane was probed with anti-HA primary antibody, followed by antimouse secondary antibody at dilutions indicated above. Bands were visualized with the ECL Western blotting detection kit.
Lipid raft fractionation
Cell membranes were fractionated as previously described (Sturm et al. 2006) using the method of Vetrivel et al. (2004). Briefly, HEK 293 cells expressing HA-prestinNN163/166AA, either untreated, treated with 10 mM MβCD, or treated with water-soluble cholesterol for 30 min at 37°C, were lysed in buffer (0.5% Lubrol WX/17A17, Serva, 25 mM Tris–HCl, pH 7.4, 150 mM NaCl, 5 mM EDTA, and 1 mM phenylmethanesulfonyl fluoride). Membranes were separated on a 5%, 35%, and 45% sucrose gradient. Twelve 1-mL fractions were collected and excess lipids removed by methanol/chloroform precipitation before the proteins were separated by 7.5% SDS-PAGE and analyzed by Western blotting as described above.
Forty-eight hours after transfection with WT HA-prestin or HA-prestinNN163/166AA, HEK 293 cells grown in 10-cm plates were either treated with MβCD or water-soluble cholesterol for 30 min or left untreated before gentle harvesting into 1 mL of PBS pH 8.0. The cells from each plate were harvested, split into eight aliquots, pelleted (2,000×g for 5 min), and each aliquot was incubated with various concentrations of cross-linker (bis(sulphosuccinimidyl)suberate [BS3]; 0 to 5 mM) for 30 min at room temperature. Reactions were quenched with 50 mM Tris pH 7.5. The samples were mixed with 8% 2X PFO sample buffer and incubated for 30 min at room temperature before separation on a 4–8% Tris–glycine gel for analysis by Western blotting.
(a) Prestin cell-surface expression: HEK 293 cells grown on coverslips were transfected with HA-prestin-IRES-GFP or HA-prestinNN163/166AA-IRES-GFP vectors. Thirty-two to 48 h post-transfection, cells were either left untreated or treated with 10 mM water-soluble cholesterol or MβCD for 30 min at 37°C. Cells were then washed with PBS, stained with concanavalin A–AlexaFluor 350 conjugate (Molecular Probes, Carlsbad, CA, USA) in PBS for 1 h on ice, and washed with PBS again before fixing with 4% paraformaldehyde in PBS. The cells were then stained with anti-HA antibody (1:1,200; Cell Signaling Technology, Denvers, MA, USA) in PBST for 1 h, followed by three PBS washes and then AlexaFluor 594 goat antimouse secondary antibody (1:800; Molecular Probes, Carlsbad, CA, USA) in PBST for 1 h prior to PBS washes. (b) Prestin in endocytic vesicles: HEK 293 cells grown on coverslips were cotransfected with either Cav1-mRFP or mRFP-clathrin and either WT or prestinNN163/166AA-mGFP. Thirty-two to 48 h after transfection, cells were either left untreated or treated with 10 mM water-soluble cholesterol for 30 min at 37°C. Cells were then washed with PBS before fixing with 4% paraformaldehyde for 10 min. GFP and RFP epifluorescence were used to visualize prestin and caveolin/clathrin, respectively. For both sets of colocalization studies: Coverslips were mounted on slides with Fluoromount G antifade reagent and fluorescence captured on a Zeiss Axioplan 2 deconvolution microscope with ×63 or ×100 objective and analyzed with the Delta Vision Applied Precision SoftWoRx software. Quantification of prestin endocytic colocalization: The colocalization of prestin with caveolin was measured using the colocalization module included in the software package, which generates a pixel-by-pixel scatter plot of the intensities of two channels and provides the Pearson's coefficient of correlation. The Pearson's coefficient is obtained by dividing the covariance of two variables by the product of their standard deviation (SD) and reflects the strength of the linear relationship between the two variables; in this case, the intensities of GFP and RFP fluorescence.
Electrophysiological data were obtained from HEK 293 cells using the whole-cell voltage-clamp technique. Cells were either voltage-clamped without treatment or treated with 10 mM MβCD or water-soluble cholesterol for 30 min at 37°C prior to voltage-clamping. For the kinetic studies, cells were first patched, then MβCD (100 µM final concentration) or water-soluble cholesterol (1 mM final concentration) was added into the external solution, and NLC was recorded at time intervals of 1 to 5 min. Our recording techniques are fully described earlier (Rajagopalan et al. 2006), but a brief description follows. Culture dishes containing transfected cells were placed on the stage of an inverted microscope (Carl Zeiss, Gottingen, Germany) under ×100 magnification and extensively perfused with the extracellular solution containing Ca2+ and K+ channel blockers (in millimolars: 100 NaCl, 20 CsCl, 20 tetraethylammonium-Cl, 10 HEPES, 2 CoCl2, 1.47 MgCl2, and 2 CaCl2) prior to recording. All recordings were conducted at room temperature (23 ± 1°C). Patch pipettes (quartz glass) with resistances ranging from 2 to 4 MΩ were fabricated using a laser-based micropipette puller (P-2000, Sutter Instrument Company, Novato, CA, USA) and filled with an intracellular solution, also containing channel blockers (in millimolars: 140 CsCl, 2 MgCl, 10 ethylene glycol tetraacetic acid, and 10 HEPES). Quartz pipettes were used as they result in lower noise levels due to the low dielectric constant, low loss factor, and extremely high volume resistivity of quartz. Cell membrane admittance was measured with the patch-clamp technique in the whole-cell mode using a DC voltage ramp with dual-frequency stimulus (Santos-Sacchi et al. 1998) from −0.14 to 0.14 V with a holding potential of 0 V, and the cell parameters were calculated from the admittance as described earlier (Farrell et al. 2006). The conductance was also determined experimentally with a DC protocol, as described earlier (Rajagopalan et al. 2006).
Fluorescence recovery after photobleaching
Fluorescence recovery after photobleaching (FRAP) experiments were performed confocally as described previously (Organ and Raphael 2007) to calculate the lateral diffusion coefficient, D, and the immobile fraction, IF, of the molecules of interest. In brief, we monitored a segment of membrane using a circular region of interest with a diameter of 2.9 µm centered along the plasma membrane. Data was collected for 410 scans with a delay of 1.5 s between scans, ∼12 min. Bleaching was performed using an argon laser at 488 nm and 100% transmission. A double-bleaching protocol was used consisting of two successive bleaches, one after scan 10 and one after scan 210, to provide additional insight into the IF. A population of truly immobile molecules will be permanently photobleached during the first bleach and will not contribute to the fluorescent signal monitored during the second bleach. Thus, the second bleach should approach complete or 100% recovery. For FRAP data analysis, the Zeiss AIM physiology software quantified intensities, and the raw data was background subtracted before each bleach was individually normalized to the average of the first ten scans (scans 200–210 for the second bleach). The normalized data was used to calculate the IF and effective diffusion coefficients, and recovery curves were individually fit in Matlab (The MathWorks, Natick, MA, USA) to the solution of a one-dimensional diffusion equation. Chauvenet's criterion was applied to the calculated D values to identify and remove outliers. Effective diffusion coefficients were then normalized to that of WT prestin.
Cell-surface biotinylation to estimate membrane prestin population
We used cell-surface biotinylation using the EZ-Link Sulfo-NHS-SS-Biotin reagent and kit (Thermo Fisher Scientific, Rockford, IL, USA) to tag and capture proteins expressed on the surface of HEK 293 cells transfected with WT prestin or prestinNN163/166AA and either left untreated or loaded with cholesterol for 30 min. Biotinylation was carried out according to the manufacturer's instructions; briefly, cells were washed with PBS, incubated with the biotin reagent for 30 min at 4°C, and quenched before being rinsed, harvested, and lysed. The total protein in the whole-cell lysate was measured using a Bradford assay and equal amounts of lysate were then passed through the streptavidin affinity column and eluted using dithiothreitol. Eluted proteins were separated by SDS-PAGE and blotted onto a nitrocellulose membrane for the detection of prestin. Band intensities on Western blots were quantitated either directly on a LI-COR Odyssey infrared imaging system using secondary antibodies supplied by the manufacturer (LI-COR Biosciences, Lincoln, NE, USA), or after chemiluminescent developing of film as described earlier in the “Methods” section using the Personal Densitometer SI with ImageQuant software (GE LifeSciences, Piscataway, NJ, USA).
Prestin is a glycoprotein; prestinNN163/166AA is nonglycosylated and functional in HEK 293 cells
We next measured the ability of prestinNN163/166AA to confer NLC, the electrical signature of electromotility, on HEK 293 cells. The NLC properties of this double mutation were earlier demonstrated to have an insignificant depolarizing shift in Vpkc, the voltage at peak capacitance, from the WT prestin value in TSA cells (Matsuda et al. 2004). We found this to also be true in HEK 293 cells where prestinNN163/166AA is fully functional and exhibited an NLC peak at approximately −55 mV, slightly depolarized from the WT value of about −70 mV (Fig. 1B). Charge density values remained unchanged between WT prestin and prestinNN163/166AA (Fig. 1B, inset).
PrestinNN163/166AA expression in the plasma membrane
PrestinNN163/166AA shows lowered self-association
Cholesterol loading ablates prestinNN163/166AA function
Prestin undergoes cholesterol-dependent changes in trafficking
PrestinNN163/166AA exhibits a lower lateral mobility and an enhanced internalization from the cell surface following cholesterol loading
N-linked glycosylation of proteins in the endoplasmic reticulum and Golgi apparatus vastly increases the structural complexity of membrane proteins. Several cellular functions have been attributed to protein glycosylation: protein folding, quality control and degradation, protein sorting, trafficking and translocation, and raft or membrane microdomain localization (Helenius and Aebi 2001). We have previously shown that membrane cholesterol modulates prestin function as well as overall auditory function (Sturm et al. 2006; Rajagopalan et al. 2007), underlining the dynamic interplay between prestin and the membrane in the generation of OHC electromotility. The involvement of N-glycosylation in protein sorting and raft interactions of some membrane proteins prompted the present investigation of the role of glycosylation in prestin function.
We have confirmed that prestin is a glycoprotein in HEK 293 cells and the double mutation of putative N-glycosylation sites results in prestinNN163/166AA being nonglycosylated, which is consistent with initial observations in other cell lines (Matsuda et al. 2004). PrestinNN163/166AA in HEK 293 cells is fully functional and has a bell-shaped NLC function similar to WT, but with a slightly depolarized peak consistent with the previous observation in CHO and TSA cells (Matsuda et al. 2004). Because N-glycosylation sites of transmembrane (TM) helices are generally positioned on the luminal or extracellular surface of membranes and the flipping of TM helices (along with their attached sugars) to an intracellular compartment are without precedent, these data minimize the proposed ten-pass TM topology model (Navaratnam et al. 2005) and lend further support for the modified 12-pass TM model for prestin (Zheng et al. 2001; Matsuda et al. 2004; Deak et al. 2005).
We observed that prestinNN163/166AA, like deglycosylated WT prestin, exists to a greater degree as monomers. Indeed, cross-linking studies indicate that higher amounts of BS3 cross-linker are required to trap prestinNN163/166AA dimers when compared to WT prestin (Rajagopalan et al. 2007), suggesting that prestinNN163/166AA molecules are more distant from each other. In addition, FRAP analysis of prestin lateral mobility in HEK 293 cells indicates that prestinNN163/166AA is more mobile in the membrane with significantly higher diffusion than WT prestin. An interpretation of these data is that prestinNN163/166AA exists predominantly in a monomeric population compared to WT prestin. This would mean that glycosylation is important for prestin self-association and oligomerization, potentially resulting from stabilizing interactions within the cell-surface glycocalyx, a known effect of glycosylation (Trombetta and Helenius 1998; Helenius and Aebi 2001; Fullekrug and Simons 2004).
Because membrane cholesterol affects prestin self-association and localization to rafts, as well as its membrane distribution and function (Rajagopalan et al. 2007), we assessed the cellular and functional consequence of a lack of glycosylation of prestin. PrestinNN163/166AA has the expected expression in HEK 293 cell membrane fractions, including membrane rafts, and cholesterol depletion did not significantly change its steady-state distribution among the different membrane fractions. These results are in contrast to cholesterol depletion of WT prestin, which redistributes out of membrane microdomains to the other membrane fractions (Sturm et al. 2006; Rajagopalan et al. 2007). Functionally, the NLC curve of prestinNN163/166AA is similar to WT prestin in untreated cells and the peak of prestinNN163/166AA NLC shifts to depolarized voltages in cells depleted of cholesterol, similar to WT behavior. These results and our findings that prestinNN163/166AA is enriched as monomers and more mobile in the plasma membrane suggest that oligomerization of prestin is not essential for the generation of NLC in HEK 293 cells in normal- and low-cholesterol environments and perhaps in the OHC lateral wall, which is low in cholesterol (Rajagopalan et al. 2007).
In contrast, the NLC of prestinNN163/166AA is markedly different from WT prestin in one aspect: loading excess cholesterol eliminates voltage-dependent capacitance in prestinNN163/166AA. Our kinetic studies indicate that this is due to a decrease in magnitude (charge density) of the NLC function and not due to an extreme shift in Vpkc. The significant decrease in linear capacitance seen upon cholesterol loading in cells expressing prestinNN163/166AA indicates a loss of cell-surface area. This, along with the drop in charge density and the decreased total level of prestinNN163/166AA expression in the plasma membrane upon cholesterol loading, is suggestive of endocytosis triggered by cholesterol loading. The loss of linear capacitance and decrease in charge density is not seen in WT, possibly due to efficient cell-surface recycling of prestin endocytic vesicles.
The main difference between WT prestin and prestinNN163/166AA appears to be in their localization in the plasma membrane and in their responses to increased membrane cholesterol, a known effector of endocytosis. One key function of N-glycosylation is in the regulation of protein sorting and trafficking (Helenius and Aebi 2001; Huet et al. 2003). Thus, an explanation for the ablation of prestinNN163/166AA NLC following cholesterol loading is that cholesterol promotes prestin endocytosis; prestinNN163/166AA should then exhibit changes in trafficking and/or be internalized more readily from high-cholesterol membranes compared to WT prestin. We, therefore, investigated the colocalization of WT prestin and prestinNN163/166AA with clathrin and caveolin in different cholesterol environments. PrestinNN163/166AA exhibits a much higher degree of colocalization with clathrin and caveolin vesicles than does WT prestin. This may explain the consistent presence of prestinNN163/166AA in all membrane fractions and/or substantiate the possibility for its increased trafficking or sorting. The high caveolin colocalization and decreased clathrin colocalization of prestinNN163/166AA in cholesterol-loading conditions indicates that it is predominantly internalized by a caveolin-dependent mechanism in high-cholesterol environments. Importantly, WT prestin also has a significant increase in colocalization with clathrin and caveolin upon cholesterol loading, indicating that prestin trafficking is dynamic and occurs via both clathrin- and caveolin-dependent mechanisms in HEK 293 cells. Further studies will be necessary to determine if prestin endocytosis is restricted to a dependence on dynamin for vesicle excision or may participate in other clathrin-independent endocytic pathways (Mayor and Pagano 2007).
Additional evidence that both WT prestin and prestinNN163/166AA participate in cholesterol-dependent endocytosis comes from measurements of lateral mobility using FRAP. There is a significantly larger IF from the first bleach for both proteins upon cholesterol loading (compared to control), suggesting increased sequestering/immobilization of prestin, as in cholesterol-induced endocytic vesicles. After the second bleach, WT prestin shows no further increase in the IF upon cholesterol loading (compared to untreated), suggesting that this endocytic pool has reached steady-state recycling. However, in the case of cholesterol loading and prestinNN163/166AA, there is still a significantly higher IF after the second bleach. This significant IF is paralleled by a concomitantly low lateral diffusion, possibly indicating that a significant fraction of prestinNN163/166AA is still being endocytosed (Kenworthy et al. 2004). PrestinNN163/166AA may be sequestered into a nonmobile membrane or juxtamembrane compartment, i.e., into caveolin vesicles or caveolin-rich membrane microdomains, reflected as loss of punctate distribution upon cholesterol loading. This conclusion is borne out by measurements of cell-surface populations of WT prestin and prestinNN163/166AA, which show a significant decrease in prestinNN163/166AA at the cell surface following cholesterol loading. Such a decrease is not seen in the case of WT prestin. These findings point to the possibility that the enriched fluorescence seen at the membrane with cholesterol loading is representative of a population just below, not at, the cell surface—possibly in endocytic vesicles triggered by cholesterol-dependent mechanisms. This explanation implies that a “threshold” amount of functional prestin is required in the membrane to generate measurable NLC; the relationship between prestin levels and magnitude of NLC has not been studied in detail to date.
In conclusion, N-glycosylation of prestin is a mechanism by which the cell regulates the level of prestin trafficking to and from the plasma membrane. Our findings correlate well with another study investigating the effect of cholesterol manipulations on the magnitude of prestin-associated charge movement (Sfondouris et al. 2008). In addition to a hyperpolarizing shift, the study demonstrates that there is an associated small decrease in charge density for WT prestin upon cholesterol loading. This decrease in charge density, unlike the peak shift, is not immediately reversible, indicating that separate mechanisms are responsible for each phenomenon. Our finding that prestinNN163/166AA undergoes cholesterol-dependent endocytosis explains the associated decrease in charge density. Our findings suggest that, both during development and during conditions of cholesterolemia or lipid storage disease states (Tami et al. 1985; Saito et al. 1986; Sikora et al. 1986; Preyer et al. 2001; Evans et al. 2006; Yanjanin et al. 2009), glycosylation of prestin would play a vital role in regulating cell-surface expression of prestin to effect and maintain normal hearing. Based on our observations, we conclude that glycosylation regulates self-association and cellular trafficking of prestin, most likely by regulating its localization to and/or internalization from membrane microdomains by clathrin- and caveolin-dependent mechanisms.
This work was funded by DC00354 (NIDCD; to WEB and FAP) and DC008134 (NIDCD; to FAP and RMR) and by a research grant from the Deafness Research Foundation to LR. LR was also supported by a NIH T90 postdoctoral training fellowship DK070121 through the Keck Center for Interdisciplinary Bioscience. LEO was supported by a NIDCD predoctoral training grant DC008058.
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