Temporal Coding by Cochlear Nucleus Bushy Cells in DBA/2J Mice with Early Onset Hearing Loss
The bushy cells of the anterior ventral cochlear nucleus (AVCN) preserve or improve the temporal coding of sound information arriving from auditory nerve fibers (ANF). The critical cellular mechanisms entailed in this process include the specialized nerve terminals, the endbulbs of Held, and the membrane conductance configuration of the bushy cell. In one strain of mice (DBA/2J), an early-onset hearing loss can cause a reduction in neurotransmitter release probability, and a smaller and slower spontaneous miniature excitatory postsynaptic current (EPSC) at the endbulb synapse. In the present study, by using a brain slice preparation, we tested the hypothesis that these changes in synaptic transmission would degrade the transmission of timing information from the ANF to the AVCN bushy neuron. We show that the electrical excitability of bushy cells in hearing-impaired old DBA mice was different from that in young, normal-hearing DBA mice. We found an increase in the action potential (AP) firing threshold with current injection; a larger AP afterhyperpolarization; and an increase in the number of spikes produced by large depolarizing currents. We also tested the temporal precision of bushy cell responses to high-frequency stimulation of the ANF. The standard deviation of spikes (spike jitter) produced by ANF-evoked excitatory postsynaptic potentials (EPSPs) was largely unaffected in old DBA mice. However, spike entrainment during a 100-Hz volley of EPSPs was significantly reduced. This was not a limitation of the ability of bushy cells to fire APs at this stimulus frequency, because entrainment to trains of current pulses was unaffected. Moreover, the decrease in entrainment is not attributable to increased synaptic depression. Surprisingly, the spike latency was 0.46 ms shorter in old DBA mice, and was apparently attributable to a faster conduction velocity, since the evoked excitatory postsynaptic current (EPSC) latency was shorter in old DBA mice as well. We also tested the contribution of the low-voltage-activated K+ conductance (gKLV) on the spike latency by using dynamic clamp. Alteration in gKLV had little effect on the spike latency. To test whether these changes in DBA mice were simply a result of continued postnatal maturation, we repeated the experiments in CBA mice, a strain that shows normal hearing thresholds through this age range. CBA mice exhibited no reduction in entrainment or increased spike jitter with age. We conclude that the ability of AVCN bushy neurons to reliably follow ANF EPSPs is compromised in a frequency-dependent fashion in hearing-impaired mice. This effect can be best explained by an increase in spike threshold.
Keywordsauditory spike reliability entrainment deafness endbulb of Held
Many biologically relevant aspects of the auditory environment require the brain to discern features of the acoustic stimulus that occur on a time scale of tens of microseconds. Cells in the central auditory pathways are specialized to retain and even improve the auditory timing information that is available from the hair cell. In the anterior ventral cochlear nucleus (AVCN), bushy cells are capable of exquisite phase-locking that, in some cases, is even greater than that of auditory nerve fibers (ANFs) (Smith et al. 1993; Joris et al. 1994; Paolini et al. 2001). To accomplish this task, bushy cells possess synaptic and biophysical specializations (Oertel 1997; Trussell 1999). ANFs give rise to large terminals, the endbulbs of Held, with multiple synaptic contacts that deliver strong synaptic drive to the bushy cell. Postsynaptic AMPA receptor-mediated synaptic currents in bushy cells decay with time constants on the order of 160 μs at 33°C (Raman and Trussell 1992; Isaacson and Walmsley 1996; Gardner et al. 1999; Brenowitz and Trussell 2001; Gardner et al. 2001). Bushy cells also have a low-voltage-activated K+ conductance (gKLV) that endows these cells with a low resting input resistance and a short membrane time constant (Oertel 1983; Manis and Marx 1991; Reyes et al. 1994; Rothman and Manis 2003b). Together, these pre- and postsynaptic specializations help ensure a high reliability of postsynaptic spiking during ANF activity, while simultaneously enforcing a short temporal window for excitatory postsynaptic potentials (EPSP) summation.
The temporal processing of sound can be affected by hearing loss. In humans, hearing loss results in poor gap detection and speech discrimination (Nelson and Thomas 1997; Snell and Frisina 2000). In mice, temporal sound processing and sound localization deteriorate in aged hearing-impaired animals (Walton et al. 1998; Heffner et al. 2001; Ison and Allen 2003). DBA mice are one model system that can be used to investigate the effect of early-onset age-related hearing loss. At 3 weeks of age, DBA mice have normal hearing threshold (Willott and Erway 1998; Zheng et al. 1999); however, by 5 weeks, hearing thresholds are elevated by ∼30 dB for frequencies above 8 kHz. We previously found that synaptic transmission at the endbulb of Held was affected by the hearing status of DBA mice, whereas no changes were seen in normal hearing CBA mice (Wang and Manis 2005). Hearing-impaired DBA mice exhibited a reduction in the frequency of spontaneous transmitter release, a decrease in quantal size, and a decrease in release probability. In addition, the decay time course of spontaneous EPSCs was slower and the voltage-dependent rectification of EPSC decreased, suggesting a change in the subunit composition of the postsynaptic AMPA receptors (Wang and Manis 2005). On the other hand, minor changes were seen in the intrinsic properties of the cells, consistent with results reported with cochlear ablation in rats and chicks (Francis and Manis 2000; Lu et al. 2004).
The changes in synaptic transmission in older DBA mice suggested that there might be a corresponding functional degradation in the ability of bushy neurons to convey high-frequency, precise temporal information to higher auditory centers. To test this hypothesis, we recorded from bushy cells while presenting shocks at a high rate to the ANF in a brain slice preparation. We measured spike latency, spike jitter and spike entrainment as well as EPSC latency and depression in the high frequency (HF) regions of the AVCN, in both 22- and 45-day-old DBA mice. We also tested bushy cells from the same regions of the AVCN in age-matched CBA mice that show no hearing loss.
MATERIALS AND METHODS
Animals and hearing tests
DBA/2J and CBA/Caj mice (Jackson Lab, Bar Harbor, ME) between 20 and 57 days were used in these experiments. All animal protocols were approved by the Institutional Animal Care and Use Committee at the University of North Carolina at Chapel Hill. DBA/2J mice older than 40 days were found to have little or no Preyer’s reflex in response to a hand clap; CBA mice had a normal reflex regardless of age. To provide an objective measure of hearing thresholds, a subset of mice were tested for auditory brainstem evoked responses (ABR) to clicks immediately prior to preparing brain slices. For these measurements, the mouse was anesthetized intraperitoneally (i.p.) with Avertin (0.15 ml/10 g wt). A small incision was made in the pinna to allow better access to the ear canal, and the mouse was placed in an acoustically isolated chamber (ENV-018M, MED Associates, St. Albans, VT, USA). The animal’s temperature was maintained at 37°C via a thermostatically controlled feedback system (Harvard Apparatus, Holliston, MA, USA). An electrostatic speaker (TDT ES-1), with a 1.5-cm-long polyethylene tube was abutted against the ear canal to make a closed acoustic system. Recording electrodes were placed at the vertex and mastoid, with a remote ground under the skin in the rump area. Signals were amplified 10,000× with a Grass-Telefactor P511J amplifier, and bandpass-filtered from 100 to 3000 Hz. ABR responses were averaged with a Tucker-Davis (Gainesville, FL, USA) System-3 RP2.1 processor controlled by a Matlab (Mathworks, Natick, MA, USA) routine. Stimuli were generated through the RP2.1 and passed through a PA5 attenuator prior to delivery to the speaker amplifier. A series of responses to clicks (0.5-ms rectangular pulses generated by the RP2.1) were collected at 5-dB intensity steps, over a 70- to 80-dB range. Click polarities were alternated on sequential trials, and the waveforms averaged. This allowed us to minimize the contribution of the cochlear microphonics to the ABR, and to focus on the components of the waveform generated by the central pathways. ABRs were analyzed by plotting the amplitude of each peak against stimulus intensity. Two independent observers then estimated the threshold as the lowest intensity at which the response was clearly discernible. Immediately after the ABR measurements, brain slices of the AVCN were prepared.
Slices of cochlear nucleus were prepared as previously described (Oertel 1983; Wang and Manis 2005). Briefly, mice were anesthetized with ketamine (100 mg/kg)/xylazine (10 mg/kg, i.p.), and decapitated. The brainstem, including the cochlear nucleus, was immediately harvested and immersed in prewarmed (34°C) dissection artificial cerebrospinal fluid (ACSF), which differed from standard ACSF by containing low Ca2+ and high Mg2+ (0.1 mM CaCl2 and 3.7 mM MgSO4). The standard ACSF contains (in mM): 122 NaCl, 3 KCl, 1.25 KH2PO4, 20 glucose, 25 NaHCO3, 2 Na pyruvate, 3 myo-inositol, 0.4 ascorbic acid, 2 CaCl2, 2 MgSO4, and was bubbled with 95% O2 and 5% CO2 to a pH of 7.4. The tissue block containing the cochlear nucleus was trimmed and mounted on a cutting block. Parasagittal sections 200–250 μm thick that included the cochlear nucleus were prepared by using a vibratome. Slices were incubated for at least 30 min at 34°C, and then secured in the recording chamber with nylon netting in flowing ACSF (3–5 ml/min).
All recordings in this paper were made from cells in the HF dorsal third of the AVCN (excluding the granule cell domain). The dorsal third of the nucleus was defined by the trajectory of the ANFs from the nerve root region (Wang and Manis 2005). Based on the mouse cochlear frequency map and its projection onto the AVCN (Ehret 1983; Berglund and Brown 1994; Muller et al. 2005), cells in the region we targeted would be expected to have best frequencies above 25 kHz.
AVCN neurons were visualized with a water immersion objective (40×) using Nomarski differential interference contrast optics on a Zeiss FS Axioskop (Zeiss, Oberbochen, Germany). To enhance image contrast in slices from older animals, we used white light, closed the field diaphragm nearly all the way, and aligned the condenser slightly eccentrically (Kachar 1985; Gardner et al. 2001). Patch electrode pipettes (3–8 MΩ) were pulled from borosilicate glass (KG-33, Garner Glass) with a Sutter P2000 puller (Sutter Instruments, San Francisco, CA, USA). Pipettes were coated with Sylgard 184 (Dow Corning, Midland, MI, USA) before use. The standard electrode solution contained (in mM): 110 K gluconate, 4 NaCl, 20 KCl, 10 HEPES, 0.2 EGTA, 4 Mg2+-ATP, 10 creatine phosphate, and 0.3 GTP. The pH was adjusted to 7.2 with KOH, and the final osmolarity was ∼295 mOsm. Evoked EPSC recordings (with the exception of spike latency test) were made with a Cs+-based electrode solution containing QX314 (Tocris Cookson, Bristol, UK), in order to minimize contamination from potassium and sodium conductances. This solution contained (in mM) 125 CsMeSO3, 15 CsCl, 5 EGTA, 10 HEPES, 4 Mg2+-ATP, 10 creatine phosphate, 0.3 GTP, and 2–5 mM QX314; the pH was adjusted to 7.2 with CsOH. In some experiments, 0.1% Alexa Fluor488 (Molecular Probes, Eugene, OR, USA) was added to aid cell visualization and identification. Except where indicated, recordings were performed at room temperature (22–25°C) using an Axopatch 200B (Axon Instruments, Foster City, CA) under the control of in-house software written in Matlab. Whole-cell access resistance was less than 15 MΩ and compensated to greater than 70% on-line with a 20-μs lag time. For dynamic clamp, addition or subtraction of the gKLV was accomplished by using the Real-Time Linux Dynamic Clamp (RTLDC, V2.2) system developed by Dorval et al. (2001). The dynamic clamp system utilized a separate acquisition computer (Intel Pentium P4, 2.8 GHz) running Linux (Fedora 3.0, RedHat, Raleigh, NC, USA) and a 16-bit National Instruments A/D-D/A card (NI6052E), in parallel with the primary acquisition system, and was operated at 20–40 kHz. The parameters of gKLV were based on the kinetic model of Rothman and Manis (2003b, c) at 22°C. We adjusted gKLV over a range of values, while examining the response of the cell to current pulses injected by the primary recording system, or to stimuli delivered to the AN. To record low-voltage-activated K+ current, 0.5 μM tetrodotoxin (Sigma, St Louis, MO, USA) and 10 μM ZD7288 (Sigma) were added to the ACSF to block voltage-gated Na+ current and hyperpolarization activated cation conductances. The addition of 30 nM α-dendrotoxin (Alomone, Jerusalem, Israel) was used to block gKLV.
EPSC threshold was measured by varying the ANF stimulus strength. The stimulus strength was then adjusted to be 1.5–2× threshold in order to elicit reliable minimal EPSCs, while at the same time avoiding activation of additional inputs to the cell. Only cells with a clear all-or-none minimal EPSCs of more than 2 nA were selected for analysis; these responses are typical of bushy cells in AVCN (Isaacson and Walmsley 1995).
To test the consequences of high-frequency deafness, responses of bushy cells to trains of auditory nerve shocks were examined in current clamp. The auditory nerve root was stimulated with a train of 20 pulses for 20 trials with intertrial interval of 5–8 seconds. The stimulus rates used were 100, 200, and 300 Hz with shock intensity at 1.5–2× response threshold.
Neuronal parameters of AVCN bushy cells in DBA and CBA mice
DBA (mean ± SE)
CBA (mean ± SE)
HF Old (n = 22)
HF Young (n = 15)
HF Old (n = 9)
HF Young (n = 10)
−64.3 ± 0.8
−66.0 ± 1.7
−65.2 ± 1.1
−64.1 ± 0.8
143.2 ± 7.7
142.5 ± 9.4
131.4 ± 19.5
139.7 ± 9.8
Membrane time constant (ms)
1.71 ± 0.14
1.85 ± 0.21
1.85 ± 0.36
1.90 ± 0.23
IV rectification (slope ratio at ± 100 pA)
0.72 ± 0.02
0.67 ± 0.04
0.66 ± 0.04
0.70 ± 0.04
AP height (mV)
53.9 ± 1.4
53.4 ± 3.2
54.13 ± 2.5
53.8 ± 2.7
AP half-width (ms)
1.36 ± 0.09
1.56 ± 0.13
1.51 ± 0.20
1.66 ± 0.16
Max rising rate of spike (mV/ms)
87.4 ± 7.6
75.7 ± 7.1
83.5 ± 12.5
86.5 ± 4.2
Max falling rate of spike (mV/ms)
44.1 ± 6.7
31.7 ± 4.3
37.9 ± 10.9
29.2 ± 10.9
6.9 ± 0.4**
4.1 ± 0.5
4.3 ± 0.9
3.6 ± 0.7
AP threshold I (pA)
166.7 ± 22.1* (n = 24)
108.0 ± 12.5 (n = 25)
161.1 ± 26.1
131.3 ± 13.2
AP # (at I = +300 pA)
5.6 ± 1.0*
3.2 ± 0.9
1.3 ± 0.2
2.1 ± 0.7
Spike latency was measured from the onset of each auditory nerve shock to the peak of the AP. The latency to the spike peak was used, rather than a threshold crossing or detection of an inflection point, because the APs changed shape and size during the stimulus train. Spike jitter (of a given pulse in the train) was calculated as the standard deviation of spike latencies over 20 repetitive trials. The entrainment index was calculated for each shock in the train as the fraction of trials on which the cell spiked (Fig 2C). Synaptic depression was calculated as the ratio of a given EPSC amplitude to the first EPSC.
Statistical significance (p < 0.05) was determined by using unpaired Student’s t-test or two-way analysis of variance (ANOVA), where appropriate, using Prism V3 (GraphPad Software, San Diego, CA, USA). All other analyses were carried out using custom scripts in Matlab. Data are shown as means ± SE.
Comparison of electrical excitability in mice with and without hearing loss
As reported previously in cochlear-ablated rats (Francis and Manis 2000), chickens (Lu et al. 2004), and in hearing-impaired DBA mice at 33°C (Wang and Manis 2005), the passive electrical properties (RMP, input resistance, and membrane time constant) of type II cells were little affected by age or hearing status (Table 1). In addition, the AP shape (as measured by the rising and falling slope, half-width, and peak height) was not statistically different between young and old DBA mice. Similarly, no differences in the passive membrane properties nor in the active electrical behavior were seen between young and old CBA mice.
However, a few aspects of the electrical excitability were different between the old, impaired-hearing and the young, normal-hearing DBA mice. First, the action potential afterhyperpolarization (AHP) was significantly deeper in old than in young DBA mice. Second, similar to that found at 33°C (Wang and Manis 2005), the current required to elicit an AP in old DBA mice was larger than that in young DBA mice. Third, although the type II cells always responded with single spikes when tested with small current pulses, larger depolarizing currents could drive multiple spikes in a subset of cells. Multiple spiking was especially predominant in old DBA mice (Table 1). Thirteen of the 22 cells in old DBA mice had more than 2 spikes in response to +300 pA current injection, whereas only 4 of 15 cells in young DBA showed >2 spikes. In contrast, only 1 of 10 young CBA and 0 of 9 old CBA cells responded with >2 spikes to the +300 pA current injection. The presence of multiple spikes with larger currents suggests a weaker low-voltage-activated K+ conductance (Brew and Forsythe 1995; Rothman and Manis 2003b, c).
We tested whether the shorter spike latency in the older DBA mice arose from an increase in axonal conduction velocity and/or a shorter synaptic delay by directly measuring the latency of evoked EPSCs in voltage clamp. In a different set of cells, we measured both spike latency (in current clamp) and the underlying EPSC (in voltage clamp) in the same cells, and found that there was a linear correlation between spike latency and EPSC latency (Fig. 3B, middle panel, r=0.72, p=0.002). The EPSC latency of high-frequency cells from young DBA was 1.73 ± 0.05 ms (n=7), whereas the latency from old DBA mice was 1.50 ± 0.06 ms (n=5, p=0.016). In contrast, EPSC amplitude was not correlated with AP spike latency (Fig. 3B, lower panel).
We examined the temporal coding capability of the AVCN bushy neurons in a mouse model of early-onset hearing loss. An increase in AP threshold along with an increase in the depth of the AP after hyperpolarization and the number of APs produced by 300 pA depolarizing current pulses were the significant changes in intrinsic membrane properties found in hearing-impaired old DBA mice. In addition, the ability of the cells to fire reliably during trains of EPSPs at 100 Hz was significantly reduced in hearing-impaired DBA mice. The latency of APs to ANF stimulation was 0.46 ms shorter than normal in old DBA mice, and this was not caused by a change in low-voltage-activated K+ conductance, but it was associated with a shorter EPSC latency. No significant change in spike jitter was observed between normal-hearing young and impaired hearing old DBA mice when spike jitter was normalized to the latency. The observed changes do not appear to be simply age-related, as there were no differences in intrinsic membrane properties, AP entrainment, or spike latency in age-matched CBA mice with normal hearing.
Despite a 20-dB increase in click ABR threshold, the age-related hearing loss in DBA mice produced some minor changes in intrinsic excitability of the HF bushy cells. The pattern of changes in electrical excitability that we observed did not match with those from previous reports, which examined hearing loss produced by cochlear ablation. For example, cochlear ablation resulted in small (but significant) changes in RMP, input resistance, action potential AHP (Francis and Manis 2000), and AP height (Lu et al. 2004). In the present study, no evidence for changes in RMP or input resistance was observed in HF cells of old, hearing-impaired DBA mice. The increase in action potential AHP was opposite to what was reported in cochlear ablated rats (Francis and Manis 2000). In addition, unlike cochlear-ablated rats (Francis and Manis 2000), the AP threshold was significantly elevated in old DBA mice in this study. This result confirms our previous observation in which recordings were made at 33°C (Wang and Manis 2005). The AP threshold elevation was also reported in acutely cochlear ablated chick NM neurons (Lu et al. 2004). Furthermore, multiple spiking of bushy neurons in response to large depolarizing currents indicates a reduction of gKLV in hearing-impaired old DBA mice. Interestingly, a similar reduction in gKLV has been reported in cochlear-ablated chick NM neurons (protein expression) (Lu et al. 2004) and in congenitally deaf mouse MNTB neurons (current density) (Leao et al. 2004). The different aspects of changes in intrinsic excitability observed in this study as compared to previous reports may reflect the different nature of “hearing loss.” The progressive hearing loss studied here should cause a gradual change in auditory nerve activity, and at these ages, does not cause synaptic degeneration (Willott and Bross 1996). Cochlear ablation, on the other hand, immediately eliminates all auditory input to the cochlear nucleus neurons, leading to severe presynaptic terminal and fiber degeneration in the cochlear nucleus (Tucci et al. 1987; Born and Rubel 1988; Born et al. 1991; Benson et al. 1997).
The systematic latency difference between young and old high-frequency bushy cells in DBA mice was quite striking. We were able to directly compare spike latency and synaptic EPSC latency in the same set of cells, and found that there was a direct correlation between EPSC latency and spike latency. No correlation of the EPSC amplitude and the spike latency was found (Fig. 3B). Thus the spike latency difference appears to be principally related to axonal conduction velocity and/or synaptic delay. The source of this conduction speed difference is not clear although it is likely attributable to continued myelination (Romand and Romand 1990); or possibly continued maturation of Na+ channels at the node of Ranvier in the auditory nerve. Interestingly, the second 10 pulses of the 100-Hz stimulation in older CBA mice showed slightly shorter latency as well. Another possibility is that the hearing loss produces a compensatory increase in Na+ channel density in the ANF, as reported after axotomy in dorsal root ganglion neurons (Cummins and Waxman 1997; Waxman et al. 1999; Abdulla and Smith 2002; Black et al. 2004).
The EPSC delay (measured at the peak) is somewhat smaller than the spike delay (also measured at the peak). Thus we also considered whether gKLV contributed to the spike delay, because our current clamp data suggest that bushy cells in hearing-impaired VCN have reduced gKLV, as deduced from the observation that cells in old DBA mice tend to fire more APs when injected with a large (300 pA) depolarizing current. The strength of gKLV can have two opposing effects for EPSP to reach spike threshold. On one hand, it can oppose the depolarizing Na+ conductance, and thus slow the rise to firing threshold. On the other hand, gKLV shortens the EPSP rise time (Rothman and Manis 2003a; Fig. 6) because it largely determines the membrane time constant at rest. Our results indicate that across a reasonable range of gKLV, spike latency was minimally affected. However, we can not rule out the possibility that the lack of gKLV effect on spike latency is attributable to remotely located gKLV that can not be affected by dynamic clamp.
The spike jitter is clearly sensitive to stimulus rate. Thus it probably reflects Na+ channel fluctuation during high rate of activity (Rubinstein 1995; Matsuoka et al. 2001) as well as jitter in the initiation of AP due to inactivation of Na+ channels in the bushy cell. The first spike jitter was very similar between young and old DBA mice (Fig. 5A). However, the overall spike jitter in old DBA mice is slightly, but significantly, less than that in young DBA mice (Fig. 5C). During early development, there is a systematic improvement of spike timing jitter in avian NM neurons (Brenowitz and Trussell 2001) and mammalian MNTB neurons (Taschenberger and von Gersdorff 2000). Thus the reduced jitter in old DBA mice could be explained as continued development. However, the data from CBA mice do not support this idea. Not only does the spike jitter not decrease between P22 and P45 in CBA mice, but it is slightly better in P22 CBA mice (Fig. 5A). The extra jitter in young DBA mice could be a result of elevated voltage-gated Na+ current fluctuation at the newly formed axon nodes of Ranvier (Rubinstein, 1995), or may simply reflect the longer spike latency in young DBA mice. When the jitter was normalized to spike latency, there was no difference between the young and old DBA mice (Fig. 5B).
Our functional analysis of the ability of bushy cells to fire reliably to repetitive auditory nerve shocks revealed differences between DBA and CBA mice. Overall, bushy cells from CBA mice appear to entrain better than those from DBA mice in all 3 shock frequencies tested. Nevertheless, although the HF bushy cells in old DBA mice retain their ability for precise spike timing, entrainment to high-frequency stimuli could not be sustained during a long stimulus train. Despite decreased entrainment, bushy cells are capable of firing APs of up to 300 Hz in response to current pulses (Fig. 6C, inset). Thus, the decreased entrainment to 100 Hz ANF stimulation in bushy cells from hearing-impaired DBA mice suggests a deterioration of the cell’s ability to follow sustained high-frequency excitatory synaptic input. The increased AP firing threshold (Table 1) (also Lu et al. 2004; Wang and Manis 2005) is likely a major contributor of the reduced entrainment. Entrainment could also be influenced by other factors, including a decrease in the input resistance in the depolarizing direction (Francis and Manis 2000; Lu et al. 2004), or the activation of a slowly developing conductance that shunts the EPSP, for example, a Cl− conductance from inhibitory inputs (Wu and Oertel 1986; Funabiki et al., 1998). However, local inhibition by D-stellate cells does not appear to be important, because application of 1 μM strychnine, which blocks glycinergic synapses in AVCN (Wu and Oertel 1986; Harty and Manis 1996), did not enhance entrainment (data not shown). We can not rule out the influence of GABAergic synaptic input, although since no inhibitory postsynaptic potentials (IPSPs) were visible in the presence of strychnine, this seems unlikely.
Evoked synaptic current amplitude, synaptic depression, or asynchronous vesicular release could play a role in shaping the response of VCN bushy cells to high-frequency afferent activity. Our data show no difference in synaptic depression between young and old DBA mice (Fig. 7). We also show that the synaptic current in old DBA mice is not different from that in young DBA mice, consistent with our previous study (Wang and Manis 2005). Thus synaptic depression does not appear to play a role in the entrainment decrease at 100 Hz. On the other hand, in spite of high safety margins for suprathreshold synaptic transmission between the ANF and bushy cell, the spike entrainment of bushy cells to nerve stimulation at 200 and 300 Hz drops to levels below 0.5 in both DBA and CBA mice. As an interesting comparison, simultaneous pre- and postsynaptic recordings from mice in vivo have suggested that about 50% of presynaptic spikes fail to trigger a postsynaptic spike (Pfeiffer 1966; Kopp-Scheinpflug et al. 2002). Although part of this failure can be attributed to inhibitory inputs, part may reflect the effect of synaptic depression.
In conclusion, we have demonstrated that the substantial hearing loss in DBA mice leads to changes in intrinsic membrane excitability of AVCN principal neurons. A functional assay revealed a reduction of the ability of bushy cells to entrain to high-frequency AN input because of increased AP firing threshold. This decrease in entrainment in high-frequency bushy cells of old DBA mice would be predicted to affect the processing of amplitude modulated sounds, which may be used as temporal cues for sound localization in mice (Henry 2000; Walton et al. 2002; Allen et al. 2006).
This study was supported by NIDCD grants R01DC04551 to PBM and F32DC04979 to Y.W.
- Allen P, Muldrow A, Rivoli PJ, John AS, Ison JR. Age-related loss of behavioral ability to track ongoing but not initial SAM envelope in CBA/Caj mice with intact modulation transfer functions. ARO Midwinter Meeting, Baltimore, MD. 2006.Google Scholar
- Cao X, Oertel D. Voltage-sensitive conductances in bushy cells of the ventral cochlear nucleus of mice. Abstract, Assoc Res Otol 29, 2006.Google Scholar
- Ehret G. Psychoacoustics. In: Willot JF (ed) The Auditory Psychobiology of the Mouse. Charles C. Thomas, Springfield, IL, 1983.Google Scholar
- Heffner RS, Gimseong K, Heffner HE. Focus: sound-localization acuity changes with age in C57BL/6J mice. In: Willot JF (ed) Handbook of Mouse Auditory Research: From Behavior to Molecular Biology. CRC Press, Boca Raton, FL, pp. 31–35, 2001.Google Scholar