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Invertebrate Neuroscience

, 18:15 | Cite as

A rapid introduction to neurological biochemistry using Drosophila melanogaster

  • Setareh S. ChongEmail author
  • Anthony J. Wilkinson
  • Sangeeta Chawla
Technical Report

Abstract

Short, cost-effective teaching activities are a useful way of providing an integrated view on biological processes. Here we describe a brief, hands-on workshop that allows pre-university students to explore their understanding of a neurological pathway from its chemical bases to phenotype. The workshop effectively introduces the students to data collection and analysis in an enjoyable way and at an appropriate level, determined by an end of session feedback survey. The design of the workshop can be adapted and scaled to generate diverse sessions such as university teaching practicals or summer school training workshops.

Keywords

Dopamine LRRK2 Parkinson’s disease Teaching Outreach 

Notes

Acknowledgements

We would like to thank Dianne Doubtfire and Nicola Charlton for technical support, Stephanie Ellis for administrative support, and Antje Kuhrs for discussions. Chris Elliott and Richard Maguire for fly strains. Natalie Armstrong for suggestion of the development of the workshop. Paul Shields for photography. Jack Munns for the idea of fly racing card. James Chong for critical reading of the manuscript.

Compliance with ethical standards

Conflict of interest

None.

References

  1. Bellen HJ, Tong C, Tsuda H (2010) 100 years of Drosophila research and its impact on vertebrate neuroscience: a history lesson for the future. Nat Rev Neurosci 11:514–522CrossRefGoogle Scholar
  2. Bilder D, Irvine KD (2017) Taking stock of the Drosophila research ecosystem. Genetics 206:1227–1236CrossRefGoogle Scholar
  3. Blaschko H (1939) The specific action of l-dopa decarboxylase. J Physiol 96:50P–51PGoogle Scholar
  4. Carpenter JM (1950) A new semisynthetic food medium for Drosophila. Drosoph Inf Serv 24:96–97Google Scholar
  5. Corti O, Lesage S, Brice A (2011) What genetics tells us about the causes and mechanisms of Parkinson’s disease. Physiol Rev 91:1161–1218CrossRefGoogle Scholar
  6. Daubner SC, Le T, Wang S (2011) Tyrosine hydroxylase and regulation of dopamine synthesis. Arch Biochem Biophys 508:1–12CrossRefGoogle Scholar
  7. Gargano JW, Martin I, Bhandari P, Grotewiel MS (2005) Rapid iterative negative geotaxis (RING): a new method for assessing age-related locomotor decline in Drosophila. Exp Gerontol 40:386–395CrossRefGoogle Scholar
  8. Garris PA, Ciolkowski EL, Pastore P, Wightman RM (1994) Efflux of dopamine from the synaptic cleft in the nucleus accumbens of the rat brain. J Neurosci 14:6084–6093CrossRefGoogle Scholar
  9. Hurst WJ, Toomey PB (1981) High-performance liquid chromatographic determination of four biogenic amines in chocolate. Analyst 106:394–402CrossRefGoogle Scholar
  10. Intra J, Pasini M (2016) The fruit fly Drosophila as a powerful tool in teaching life sciences in middle and high school classrooms. Int J Innov ResGoogle Scholar
  11. Kalia LV, Lang AE (2015) Parkinson’s disease. Lancet 386:896–912CrossRefGoogle Scholar
  12. Kelley P, Whatson T (2013) Making long-term memories in minutes: a spaced learning pattern from memory research in education. Front Hum Neurosci 7:589CrossRefGoogle Scholar
  13. Lee SB, Kim W, Lee S, Chung J (2007) Loss of LRRK2/PARK8 induces degeneration of dopaminergic neurons in Drosophila. Biochem Biophys Res Commun 358:534–539CrossRefGoogle Scholar
  14. Lessing D, Bonini NM (2009) Maintaining the brain: insight into human neurodegeneration from Drosophila melanogaster mutants. Nat Rev Genet 10:359–370CrossRefGoogle Scholar
  15. McNicholas S, Potterton E, Wilson KS, Noble MEM (2011) Presenting your structures: the CCP4mg molecular-graphics software. Acta Crystallogr D Biol Crystallogr 67:386–394CrossRefGoogle Scholar
  16. Meiser J, Weindl D, Hiller K (2013) Complexity of dopamine metabolism. Cell Commun Signal 11:34CrossRefGoogle Scholar
  17. Quintero-Espinosa D, Jimenez-Del-Rio M, Velez-Pardo C (2017) Knockdown transgenic Lrrk Drosophila resists paraquat-induced locomotor impairment and neurodegeneration: a therapeutic strategy for Parkinson’s disease. Brain Res 1657:253–261CrossRefGoogle Scholar
  18. Robbins TW (2003) Dopamine and cognition. Curr Opin Neurol 16(Suppl 2):S1–S2CrossRefGoogle Scholar
  19. Tan S, Hermann B, Borrelli E (2003) Dopaminergic mouse mutants: investigating the roles of the different dopamine receptor subtypes and the dopamine transporter. Int Rev Neurobiol 54:145–197CrossRefGoogle Scholar
  20. Wang D, Tang B, Zhao G et al (2008) Dispensable role of Drosophila ortholog of LRRK2 kinase activity in survival of dopaminergic neurons. Mol Neurodegener 3:3CrossRefGoogle Scholar
  21. Wang S, Wacker D, Levit A et al (2017) D4 dopamine receptor high-resolution structures enable the discovery of selective agonists. Science 358:381–386CrossRefGoogle Scholar
  22. Williams TE (1986) Optimizing student-institution fit. New Directions for Higher Education 1986:35–46CrossRefGoogle Scholar
  23. Wise RA (2005) Forebrain substrates of reward and motivation. J Comp Neurol 493:115–121CrossRefGoogle Scholar
  24. Yorke M, Longden B (2008) The first-year experience of higher education in the UK. Higher Education Academy, YorkGoogle Scholar

Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Departments of BiologyUniversity of YorkYorkUK
  2. 2.Departments of ChemistryUniversity of YorkYorkUK

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