Cloning, localization and bioinformatics analysis of a gene encoding an odorant-binding protein (OBP) in Anoplophora glabripennis (Motschulsky)
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Anoplophora glabripennis (Motschulsky) has an advanced and complicated olfactory system to identify hosts, mates and spawning locations, and odorant-binding proteins (OBPs) play a key role by binding to volatile materials from different hosts. The full-length cDNA sequence of an OBP, AglaOBP, was cloned by RACE from an antenna cDNA library, and the protein structure and function were predicted by bioinformatics analysis. Gene temporal and spatial expression was detected by real-time qPCR. AglaOBP had distinctive sequence, location and expression profiles compared with other OBPs of A. glabripennis, as it was found in different tissues, and the highest expression was in the elytrums. The possible physiological functions of this OBP were discussed. These findings help elucidate the physiology of this pest and provide a new potential target for pest control.
KeywordsAnoplophora glabripennis (Motschulsky) Odorant-binding protein Gene cloning Tissue localization
Thanks for the technical support of the Key Laboratory of Genetic Resources of Forest and Forest Protection of Hebei. And Thanks for AJE’s help in language modification about this article. This work was supported by the Hebei Province Natural Science Foundation—Youth Foundation (Grant No. C2012204098).
Yu Li and Huien Li were equally contributed to the work.
Compliance with ethical standards
Conflict of interest
All authors declare that they have no conflict of interests.
The datasets generated and analysed during the current study are available from the corresponding author on reasonable request.
- Chen L, Li HL, Zhou YX et al (2013) cDNA cloning, tissue expression and ligand binding characteristics of odorant-binding protein 2 from the oriental fruit fly, Bactrocera dorsalis (Diptera: Tephritidae). Acta Entomol Sin 56(6):612–621Google Scholar
- Cheng XJ, Cai LJ, Zheng LS et al (2016) Cloning, expression profiling and binding characterization of the OBP2 gene in the diamondback moth, Plutella xylostella (Lepidoptera: Plutellidae). Acta Entomol Sin 59(4):365–376Google Scholar
- Ji T, Shen F, Liang Q et al (2014) Cloning, prokaryotic expression and tissue expression profiling of an OBP3 gene in the Chinese honeybee, Apis cerana cerana (Hymenoptera: Apidae). Acta Entomol Sin 57(8):897–904Google Scholar
- Qian K, Feng B, Wu XL et al (2015) Cloning, sequence analysis, tissue expression profiling and prokaryotic expression of odorant binding protein genes MaltOBP2 and MaltOBP6 from Monochamus alternatus (Coleoptera: Cerambycidae). Acta Entomol Sin 58(5):496–506Google Scholar
- Qin N, Yin B, Wang N et al (2007) Biologic effects of the transfected Transmembrane TNF-alpha-intracellular domain on human breast tumor cell line MCF-7. Immunological J 618:610–613Google Scholar
- Sun H, Shu M, Zhao LX et al (2013) Homology modeling of pheromone binding protein of Pectinophora gossypiella. J Environ Entomol 35(6):749–753Google Scholar
- Wang Z, Huang D, Yan J et al (2005) Occurrence dynamics of Anoplophora glabripennis in Northeastern China. J Northeast For Univ 33(6):79–82Google Scholar
- Wang JL, Zhang X, Tai FD (2009) Structure of Brain in Anoplophora glabripennis. Sichuan J Zool 28(1):82–84Google Scholar
- Wei D, Ye ZF, Gao JQ et al (2013) Molecular cloning and functional identification of a minus-C odorant binding protein from the rice striped stem borer, Chilo suppressalis (Lepidoptera: Pyralidae). Acta Entomol Sin 56(7):754–764Google Scholar
- Wu Z, Lin J, Zhang H et al (2016) BdorOBP83a-2 mediates responses of the Oriental Fruit Fly to semiochemicals. Front Physiol 7(320):1–5Google Scholar
- Zhang S (2015) Identification and expression profile analysis of odorant binding proteins genes based on transcriptome in grasshopper, Oedaleus asiaticus. J Inner Mong Agric Univ 2015:31–32Google Scholar
- Zhang ZK, Zhang Y, Sheng-Yong WU et al (2017a) Recent advances in odorant binding proteins of insects. J Environ Entomol 39(03):713–720Google Scholar
- Zhao H, Gao Y, Ma X et al (2016) Ultrastructure of Tarsal Sensilla of the Asian longhorned beetle Anoplophora glabripennis (Motschulsky) (Coleoptera: Cerambycidae). Chin Agric Sci Bull 32(17):40–43Google Scholar