Cephalopod biology and care, a COST FA1301 (CephsInAction) training school: anaesthesia and scientific procedures
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Cephalopods are the sole invertebrates included in the list of regulated species following the Directive 2010/63/EU. According to the Directive, achieving competence through adequate training is a requisite for people having a role in the different functions (article 23) as such carrying out procedures on animals, designing procedures and projects, taking care of animals, killing animals. Cephalopod Biology and Care Training Program is specifically designed to comply with the requirements of the “working document on the development of a common education and training framework to fulfil the requirements under the Directive 2010/63/EU”. The training event occurred at the ICM-CSIC in Barcelona (Spain) where people coming from Europe, America and Asia were instructed on how to cope with regulations for the use of cephalopod molluscs for scientific purposes. The training encompasses discussion on the guidelines for the use and care of animals and their welfare with particular reference to procedures that may be of interest for neuroscience. Intensive discussion has been carried out during the training sessions with focus on behavioural studies and paradigms, welfare assessment, levels of severity of scientific procedures, animal care, handling, transport, individual identification and marking, substance administration, anaesthesia, analgesia and humane killing.
KeywordsDirective 2010/63/EU Cephalopods COST Action FA1301 Training and education
The authors would like to express gratitude to ICM-CSIC staff, especially to Dr. Roger Villanueva and Dr. Mercedes Blázquez, who provided enthusiastic support and never-fatiguing contributions to logistics and all aspects of the training programme. Without support and help of Dr Mercedes Blázquez this Training School would not be possible. Trainees are also grateful to CephsInAction and CephRes Team for their tireless job, enabling this Training School to run smoothly. Authors are grateful to the three Contributing Authors that provided continuous mentoring and facilitated this note.
The COST Action FA1301 CephsInAction, and the Association for Cephalopod Research supported the Training School “Cephs Biology and Care (CBC-ASP2016)” and the participation of 10 Trainers and 14 Trainees. Sponsorships from Leica and Tecniplast has also been granted.
Compliance with ethical standards
Conflict of interest
The authors declare that they have no conflicts of interest.
The procedures carried out during the Training School CBC-ASP2016 have been authorized by the National Competent Authority of Spain [DAAM 9011 “Estudio de comportamiento y extracción de hemolinfa en cefalópodos: Sepia officinalis (sepia) y Octopus vulgaris (pulpo)”]. The Training School is largely inspired by the Guidelines for care and welfare for cephalopods in Research (Fiorito et al. 2015) and the principles stated in the Directive 2010/63/EU.
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